Introduction
If you’re working in pharmaceuticals, biotechnology, or any field that demands precise quantification of chemical compounds, you’ve probably heard of qNMR calculation. But what makes it so essential? At ResolveMass Laboratories Inc., we specialize in this advanced technique—not just to run the numbers, but to provide scientifically sound, regulator-ready results you can act on.
qNMR (Quantitative Nuclear Magnetic Resonance) isn’t just another analytical tool—it’s one of the most accurate and reliable methods available today for determining the exact amount of a substance in a sample. What sets our qNMR calculation services apart is our focus on both the science and the application. Whether you’re validating a new API, analysing an excipient, or ensuring the integrity of a reference standard, we’re here to help you get results you can trust, every time.
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What is qNMR Calculation?
qNMR calculation uses the integration of NMR signals to determine the precise quantity of an analyte relative to a standard compound. Unlike other quantification methods, qNMR calculation does not require compound-specific calibration curves, which reduces errors and improves accuracy, especially for complex matrices.
Our scientists utilize state-of-the-art NMR instruments and validated protocols that align with regulatory standards (ICH, USP, Ph. Eur) to provide dependable qNMR calculation services.
Why qNMR Calculation Services Are Crucial
1. Absolute Quantification
qNMR calculation provides a direct measurement of compound purity and concentration.
2. High Accuracy & Reproducibility
Unlike UV or HPLC, qNMR calculation offers better linearity and fewer matrix interferences.
3. Non-destructive Analysis
The sample remains intact, allowing further use or verification.
4. Cross-Industry Application
Ideal for APIs, reference standards, excipients, and natural products.
5. Regulatory Acceptance
Recognized by USP <761> and ICH Q2 for quantitative testing.
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ResolveMass Laboratories Inc.: Your Trusted qNMR Partner
At ResolveMass, we don’t just offer qNMR calculation—we offer scientifically validated results with practical impact. Our team includes PhD-level chemists with decades of combined experience in NMR spectroscopy. With a strong track record across North America and a purpose-built laboratory infrastructure, we ensure:
- Accurate quantification down to trace levels
- 1H and 13C qNMR for broader applicability
- Validated internal standards
- Full data integrity and audit trails
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Key Features of Our qNMR Calculation Services
Customized Method Development
Each project is unique. We tailor qNMR calculation protocols based on your sample’s chemical nature and regulatory requirements.
Full Validation & Documentation
You receive a complete validation package meeting ICH, USP, and other international standards.
Rapid Turnaround Times
Time is money. We deliver detailed reports within 5–10 business days without compromising accuracy.
Data Interpretation Support
Our experts don’t just send raw data—they walk you through the results and help integrate findings into your quality systems.
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Case Study: Accurate qNMR Calculation for a Generic API
Client: A mid-sized pharmaceutical manufacturer from Ontario, Canada
Objective: To validate the purity of an in-house synthesized API for submission to Health Canada.
Approach:
- qNMR calculation performed using 1H NMR with maleic acid as the internal standard.
- Sample analyzed in DMSO-d6 with known mass of standard and analyte.
- Validation included linearity (R² = 0.9998), precision (RSD < 1.5%), and accuracy (>99.5%).
Outcome:
- Confirmed API purity as 98.6%, in compliance with pharmacopeial specifications.
- Enabled client to submit regulatory documentation ahead of schedule.
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Why Scientists Trust ResolveMass for qNMR Calculation
- Unmatched Accuracy: We minimize variability by using primary standards and rigorous calibration.
- Regulatory-Ready Reports: Fully GMP-compliant data formats.
- Confidentiality Guaranteed: NDAs and client-specific data protocols.
- Ongoing Support: Post-analysis consulting and troubleshooting available.
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Frequently Asked Questions (FAQs)
1. What is the principle behind qNMR calculation?
qNMR calculation relies on the principle that the area under an NMR peak is proportional to the number of nuclei contributing to that signal. By comparing the analyte’s signal to a known internal standard, precise quantification is achieved.
2. Which internal standards are commonly used?
Standards like maleic acid, dimethyl sulfone, and benzoic acid are frequently used due to their chemical stability, solubility, and well-separated signals.
3. Is qNMR better than HPLC for quantification?
qNMR calculation offers absolute quantification without requiring calibration curves, making it more versatile for pure substances. However, HPLC might be better for complex mixtures.
4. Can qNMR be used for mixtures?
Yes, with proper peak separation and integration, qNMR calculation can quantify multiple components within a mixture.
5. What solvents are suitable for qNMR calculation?
Deuterated solvents such as DMSO-d6, CDCl3, and D2O are commonly used, selected based on solubility and chemical compatibility.
6. What is the turnaround time for qNMR services?
Typically 5–10 business days, depending on complexity and validation needs.
7. Is your qNMR service GMP-compliant?
Yes, our qNMR calculation services are compliant with Good Manufacturing Practices and include full documentation.
8. Can you help with regulatory submissions?
Absolutely. Our reports are designed for seamless integration into CTD dossiers for FDA, EMA, and Health Canada.
9. How sensitive is your qNMR system?
We can detect down to sub-milligram levels with precision, thanks to our high-field NMR instruments.
10. Do you offer consulting for method development?
Yes. We provide end-to-end consulting from method development to data interpretation and regulatory strategy.
Conclusion
When it comes to accurate, reliable, and compliant qNMR calculation services, ResolveMass Laboratories Inc. stands out as a partner scientists can trust. Whether you’re validating an API, confirming excipient purity, or developing new formulations, our qNMR calculation services deliver the data you need—accurately and on time. Let us help you meet your analytical challenges with confidence.
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ResolveMass Laboratories Inc.: Comprehensive Scientific Expertise You Can Rely On
ResolveMass Laboratories Inc. is a trusted Canadian contract research organization offering a wide spectrum of specialized services spanning polymer synthesis, advanced analytical testing, and custom organic synthesis. With over a decade of experience supporting pharmaceutical, biotech, and industrial clients, we bring scientific precision and regulatory insight to every project.
Our core capabilities include Polymer Synthesis and Characterization, Peptide Characterization, Organic Synthesis, Nitrosamine Testing and Analysis, PFAS Testing, and Extractable & Leachable Studies, as well as a broad suite of analytical techniques such as HPLC, GC-MS, MALDI-TOF, NMR, and FTIR.
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📞 Request a quote for method development
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References
- United States Pharmacopeial Convention. (2021). USP <761> Nuclear Magnetic Resonance Spectroscopy. In United States Pharmacopeia and National Formulary (USP-NF). https://www.uspnf.com
- Bharti, S., & Roy, R. (2012). Quantitative ¹H NMR spectroscopy. TrAC Trends in Analytical Chemistry, 35, 5–26. https://doi.org/10.1016/j.trac.2012.02.007
- Holzgrabe, U., Deubner, R., Schollmayer, C., & Waibel, B. (2005). Quantitative NMR spectroscopy—Applications in drug analysis. Journal of Pharmaceutical and Biomedical Analysis, 38(5), 806–812. https://doi.org/10.1016/j.jpba.2005.03.004
- International Conference on Harmonisation (ICH). (2005). Validation of analytical procedures: Text and methodology Q2(R1). https://www.ich.org/page/quality-guidelines
- Malz, F., & Jancke, H. (2005). Validation of quantitative NMR. Journal of Pharmaceutical and Biomedical Analysis, 38(5), 813–823. https://doi.org/10.1016/j.jpba.2005.01.006
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