Introduction
At ResolveMass Laboratories Inc., our Size Exclusion Chromatography Service is designed to deliver precise and reliable results for protein purification, peptide separation, and molecular weight analysis. This powerful technique is widely applied in pharmaceuticals, biotechnology, and polymer research because it ensures accuracy while preserving the structural integrity of sensitive molecules. By combining advanced technology with the knowledge of our expert scientists, we consistently provide dependable data for both routine testing and complex analytical challenges. Our methods follow international quality guidelines, making our services suitable for research institutions, biotech companies, and pharmaceutical manufacturers.
Quick Overview of This Article
- What Size Exclusion Chromatography is and its importance
- Applications in protein, peptide, and polymer research
- Key benefits of choosing ResolveMass SEC solutions
- Step-by-step explanation of our process
- Challenges in SEC and how we address them
- Comparison of SEC with other chromatography techniques
- Frequently asked questions for better understanding
What is Size Exclusion Chromatography Service?
Size Exclusion Chromatography (SEC) is a method used to separate molecules based on their size. Molecules travel through a column filled with porous beads; large molecules elute faster as they bypass the pores, while smaller ones enter the pores and take longer to pass through. At ResolveMass, we apply this technique to achieve accurate protein purification, peptide fractionation, and polymer molecular weight measurement.
Our Size Exclusion Chromatography Service is widely valued because it is non-destructive, highly reproducible, and capable of maintaining the original structure of biomolecules. This makes it especially useful in pharmaceutical development, where precision and sample purity are essential.
Why Choose ResolveMass for Size Exclusion Chromatography Service?
When you choose ResolveMass, you benefit from:
- High Precision & Accuracy – Advanced chromatographic instruments deliver consistent and reproducible results.
- Experienced Scientists – Our team has extensive expertise in protein, peptide, and polymer analysis.
- Regulatory Compliance – We follow FDA, EMA, and ICH guidelines for every analysis.
- Customized Solutions – Flexible workflows for small molecules, large biomolecules, and polymers.
- Integrated GPC Support – Correlation with Gel Permeation Chromatography (GPC) molecular insights.
Beyond instrumentation, we offer full project consultation. Clients receive clear communication, detailed reports, and reliable data tailored to their research or regulatory needs.
Applications of Size Exclusion Chromatography Service
Protein Purification
SEC is a proven method for isolating proteins from complex biological mixtures. Our laboratory ensures proteins remain stable and free from aggregates during purification. This makes our service ideal for monoclonal antibody studies, enzyme analysis, and therapeutic protein production. By preserving protein structure, we generate results that directly support drug development and quality control.
Peptide Purification
Peptide research demands accurate size-based separation and contaminant-free fractions to ensure reliable results. At ResolveMass, our SEC workflow provides precise peptide fractionation while safeguarding them from degradation. The method remains fully compatible with downstream assays and also integrates smoothly with GPC analysis for drug delivery polymers, making it especially valuable for advanced pharmaceutical applications. This approach supports biomedical research, peptide drug development, and the design of effective delivery systems.
Molecular Weight Estimation
For polymers and biomolecules, SEC is one of the most reliable techniques for estimating molecular weight and distribution. At ResolveMass, we provide precise values such as number-average molecular weight (Mn), weight-average molecular weight (Mw), and the polydispersity index (PDI). These results are further supported through Methods of Measuring Polymer Molecular Weight by GPC, ensuring accuracy and consistency. Such measurements play a vital role in materials science, product development, and regulatory submissions where uniformity and reproducibility are crucial.
Workflow of Our Size Exclusion Chromatography Service
Our SEC workflow is carefully designed to ensure accuracy and reproducibility:
- Sample Preparation – Following GPC Sample Preparation Guide for optimized loading.
- Column Selection – Choosing the right stationary phase based on GPC Column and Detector Selection.
- Run Execution – High-precision chromatography with automated detection.
- Data Analysis – Calculating molecular weights using Molecular Weight by GPC.
- Final Reporting – Detailed, regulatory-compliant reports for your project.
Each step is monitored closely, minimizing variability and ensuring every project meets international quality standards.
Advantages of Our Size Exclusion Chromatography Service
- Non-destructive method that protects molecular integrity
- Reliable results across proteins, peptides, and polymers
- Wide molecular weight detection range (500–10⁷ Da)
- Integration with GPC for polymer quality testing
- Fast turnaround times and tailored reporting
These benefits make SEC one of the most trusted methods in both academic research and industrial product development.
Challenges in Size Exclusion Chromatography and How We Solve Them
- Column Overloading – Prevented with optimized sample injection volumes.
- Non-Ideal Separation – Solved by selecting columns with the right pore size and material.
- Calibration Errors – Enhanced with GPC Errors in Polymer Molecular Weight Analysis guidance
Our proactive troubleshooting ensures high-quality data, even when handling complex biological or polymer samples.
SEC Compared to Other Chromatography Methods
| Feature | Size Exclusion Chromatography | Ion Exchange Chromatography | Affinity Chromatography |
|---|---|---|---|
| Separation Basis | Molecular Size | Charge | Specific Binding |
| Best Use | Proteins, peptides, polymers | Charged biomolecules | Tagged proteins |
| Non-Destructive | Yes | No | No |
| Application in Pharma | Molecular weight, QC testing | Protein charge studies | Target purification |
This comparison highlights why SEC is preferred when maintaining sample structure and activity is important.
Conclusion
At ResolveMass Laboratories Inc., we provide reliable and regulation-compliant Size Exclusion Chromatography Services for proteins, peptides, and polymers. Our expertise, validated workflows, and integration with GPC ensure clients receive accurate and reproducible results every time. Whether you are conducting academic research or industrial product development, our team delivers clarity, confidence, and scientific precision. Contact us today to discuss your project and see how our SEC services can support your success.
FAQs on Size Exclusion Chromatography Service
The main purpose of SEC is to separate molecules according to their size while keeping their structure unchanged. This makes it especially useful for protein purification, peptide studies, and determining polymer molecular weights with accuracy.
Unlike ion exchange or affinity chromatography, which depend on charge or specific binding, SEC relies only on the physical size of molecules. This approach makes it both reproducible and gentle, ensuring that biomolecules remain intact throughout the process.
Yes, SEC can provide highly accurate values for Mn, Mw, and PDI when proper calibration is applied. At ResolveMass, we follow internationally accepted standards to ensure reliable measurements every time.
Absolutely. SEC allows peptides of different sizes to be purified without changing their biological activity. This makes it a dependable method for peptide research, therapeutic development, and related biomedical studies.
We achieve accuracy by using validated protocols, carefully selecting detectors, and applying advanced calibration techniques. These measures help reduce errors and provide clients with consistent, reproducible data.
Yes, SEC columns are designed to analyze molecules ranging up to several million Daltons. This makes the method suitable not only for proteins but also for large polymers and other macromolecules.
Yes, all SEC services at ResolveMass follow FDA, EMA, and ICH guidelines. This ensures that results are not only accurate but also suitable for regulatory submissions in the pharmaceutical industry.
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References
- Cao, H., & Perkins, D. D. (2012). Size-exclusion chromatography of proteins. In D. Walls & S. T. Loughran (Eds.), Protein chromatography: Methods and protocols (pp. 117–124). Humana Press. https://doi.org/10.1007/978-1-61779-924-4_9
- Choudhary, S., & Kumari, N. (2013). Size exclusion chromatography in biotech industry. Research & Reviews: Journal of Pharmaceutical Analysis, 2(1), 1–10. Retrieved from https://www.rroij.com/open-access/sizeexclusion-chromatography-in-biotech-industry.php?aid=34545
- Porath, J., Flodin, P., & Granath, K. (1964). Gel filtration of proteins, peptides, and amino acids. Journal of the American Chemical Society, 86(4), 829–834. https://doi.org/10.1021/ja01059a002
- Bhargav, R. (2022). Size exclusion chromatography: A review. International Journal of Creative Research Thoughts, 10(4), 1196–1202. Retrieved from https://ijcrt.org/papers/IJCRT2204145.pdf
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