4-Chlorophenol-2,3,5,6-d4 | CAS 285132-91-4

4-Chlorophenol-2,3,5,6-d4 | CAS 285132-91-4

Overview
4-Chlorophenol-2,3,5,6-d4 is a deuterium-labeled isotopologue of 4-chlorophenol, a compound extensively used in environmental, toxicological, and analytical chemistry research. In this stable-isotope version, four hydrogen atoms on the aromatic ring are substituted with deuterium atoms (D), increasing its molecular mass by four units while maintaining identical chemical reactivity and chromatographic behavior. This makes it a highly reliable internal standard or isotopic tracer in quantitative mass spectrometry and validation studies.

ResolveMass Laboratories Inc. supplies 4-Chlorophenol-2,3,5,6-d4 with verified isotopic enrichment and chemical purity. Each batch is accompanied by a comprehensive Certificate of Analysis (CoA) that details isotopic incorporation, purity, physical characteristics, and storage recommendations. Manufactured under stringent quality control standards, this product ensures consistent analytical accuracy and reproducibility.

Chemical Information
• Product name: 4-Chlorophenol-2,3,5,6-d4
• CAS number: 285132-91-4
• Molecular formula: C6D4ClOH
• Molecular weight: 132.58 g/mol
• Synonyms: p-Chlorophenol-d4, 1-Chloro-2,3,5,6-tetradeutero-4-hydroxybenzene
• Isotopic enrichment: typically ≥98 atom % D
• Purity: typically ≥98% (verified by GC, LC, or NMR)

Applications of 4-Chlorophenol-2,3,5,6-d4 | CAS 285132-91-4
4-Chlorophenol-2,3,5,6-d4 is used across various scientific and industrial disciplines where precision in quantitation and identification of chlorophenolic compounds is required. Its stable isotope labeling ensures accurate mass differentiation without altering chemical performance.

• Environmental Analysis – Used for quantitative determination of 4-chlorophenol and related halogenated phenols in environmental matrices such as water, soil, sediments, and air particulates. The deuterated form serves as an internal standard in isotope-dilution mass spectrometry, compensating for matrix effects and extraction losses.

• Analytical Method Validation – Employed in developing and validating GC-MS and LC-MS methods for environmental and industrial testing laboratories. The labeled analogue supports calibration stability and ensures reproducibility in quantitative analysis.

• Metabolic and Toxicological Studies – Used in biochemical and toxicology research to track the biotransformation and detoxification of chlorophenols in biological systems. The isotope label allows precise discrimination between exogenous analytes and endogenous compounds.

• Industrial Quality Control – Useful for monitoring trace chlorinated phenolic impurities in chemical synthesis and manufacturing processes, ensuring compliance with product purity standards.

• Academic Research – Frequently used in kinetic isotope effect studies and NMR spectroscopy to explore molecular interactions, reactivity, and solvent dynamics of substituted phenolic compounds.

Analytical and Quality Assurance
Each batch of 4-Chlorophenol-2,3,5,6-d4 undergoes rigorous quality testing to ensure it meets or exceeds analytical-grade standards. A detailed Certificate of Analysis (CoA) accompanies every shipment and typically includes the following data:

• Isotopic enrichment confirmed by high-resolution mass spectrometry (HRMS)
• Chemical purity confirmed by GC, HPLC, or NMR analysis
• Appearance and physical description
• Water content measured by Karl Fischer titration (if applicable)
• Recommended storage and handling instructions
• Batch number and production traceability information

Typical Specifications (Representative Values)
• Isotopic enrichment: ≥98 atom % D
• Chemical purity: ≥98%
• Appearance: Off-white to pale crystalline solid

Handling
• Moisture sensitivity: Avoid extended exposure to humidity. Use under inert atmosphere for prolonged handling.
• Light sensitivity: Not significantly affected by light, but best stored in amber containers or in darkness.
• Safety precautions: Handle using appropriate laboratory personal protective equipment including gloves, lab coat, and eye protection. Avoid inhalation, ingestion, or skin contact.
• Disposal: Dispose of waste and residues according to local environmental regulations. Refer to the Safety Data Sheet (SDS) for detailed guidance.

Preparation and Analytical Use
For isotope-dilution assays, dissolve 4-Chlorophenol-2,3,5,6-d4 in a suitable solvent such as methanol, acetonitrile, or dichloromethane. Prepare calibration and working standards gravimetrically for accuracy. Introduce the labeled standard early in the analytical workflow (before extraction) to correct for losses during sample processing.

For GC-MS applications, derivatization with silylating or acylating agents may enhance volatility and chromatographic resolution. For LC-MS workflows, the compound can typically be analyzed without derivatization depending on solvent compatibility and detection sensitivity.

Packaging and Availability
ResolveMass Laboratories offers 4-Chlorophenol-2,3,5,6-d4 in various package sizes to suit research and industrial needs, from small-scale analytical vials to multi-gram bulk quantities. Custom labeling, pre-dissolved solutions, and special bulk requests are available. Every shipment includes batch-specific CoA and SDS documentation for regulatory compliance and traceability.

Regulatory and Shipping Information
This material is intended exclusively for laboratory research and analytical use. It is not for human or veterinary consumption. ResolveMass Laboratories ensures full compliance with IATA, TDG, and international shipping regulations. All shipments are securely packaged and quality-inspected before dispatch to maintain product integrity during transit.

Learn about, Deuterated polymers as guided.

Learn More about, Deuterated polymers.