Butyric-d7-acid | CAS 73607-83-7

OVERVIEW  of  Butyric-d7-acid | CAS 73607-83-7

Butyric-d₇-acid (CAS 73607-83-7) is a fully deuterated analog of butyric acid (C₄H₈O₂), in which seven hydrogen atoms are replaced by deuterium. This isotopic substitution provides a precise mass shift while maintaining the same chemical reactivity and physical characteristics as the non-labeled compound. The high isotopic purity of Butyric-d₇-acid makes it an essential internal standard and tracer molecule in advanced mass spectrometry, metabolomic, and pharmacokinetic studies.

As a deuterated short-chain fatty acid (SCFA), Butyric-d₇-acid is widely employed in research exploring gut microbial metabolism, nutritional biochemistry, cellular energy regulation, and epigenetic mechanisms. Its deuterium labeling allows accurate quantification and metabolic tracking of butyrate pathways in both in vitro and in vivo systems, supporting fields ranging from clinical diagnostics to environmental and food sciences.

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CHEMICAL INFORMATION

• Name: Butyric-d₇-acid

• Synonyms: Butanoic-d₇-acid

• CAS Number: 73607-83-7

• Molecular Formula: C₄D₇O₂

• Molecular Weight: 95.15 g/mol

• Isotopic Enrichment: ≥98 atom % D

• Solubility: Miscible with alcohols, ethers, and organic solvents; slightly soluble in water

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APPLICATIONS  of  Butyric-d7-acid | CAS 73607-83-7

1. Stable Isotope-Labeled Internal Standard
Butyric-d₇-acid serves as a stable isotope-labeled reference standard in GC-MS and LC-MS/MS assays, allowing precise quantification of native butyric acid in biological fluids such as plasma, feces, urine, and tissue extracts. Its identical chromatographic behavior but distinguishable mass enables accurate calibration and minimizes analytical errors caused by sample variability.

2. Metabolic and Nutritional Research
As a deuterated analog of a key microbial metabolite, Butyric-d₇-acid is integral to gut microbiome and metabolic flux studies. Researchers use it to trace the biosynthesis, absorption, and oxidation of butyrate in host systems, shedding light on energy metabolism, intestinal health, and the interplay between diet and microbiota.

3. Epigenetic Studies
Butyric acid is a well-known histone deacetylase (HDAC) inhibitor, capable of modulating gene expression and promoting differentiation and apoptosis in cells. The deuterated form, Butyric-d₇-acid, enables the tracing of isotopically labeled butyrate incorporation in histone acetylation studies, facilitating the exploration of epigenetic regulation mechanisms.

4. Pharmaceutical and Clinical Analysis
Used in bioanalytical method validation and drug metabolism studies, Butyric-d₇-acid supports precise measurement of SCFA levels and their correlation with metabolic diseases, gastrointestinal disorders, and therapeutic interventions.

5. Food and Environmental Testing
This compound is also utilized as a quantitative calibration standard in food chemistry and fermentation analysis for determining volatile fatty acids in dairy, beverage, and environmental samples.

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ADVANTAGES  of  Butyric-d7-acid | CAS 73607-83-7

• High Isotopic Purity (≥98 atom % D): Guarantees reliable isotopic resolution and reproducibility.

• Non-Radioactive and Stable: Safe to handle, store, and use under routine laboratory conditions.

• Mass Spectrometric Compatibility: Ideal for GC-MS and LC-MS/MS quantitative applications.

• Biochemical Fidelity: Mirrors the native butyric acid’s reactivity, polarity, and retention behavior.

• Versatile Use: Suitable for studies in metabolomics, epigenetics, pharmacokinetics, and microbiology.

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RESEARCH SIGNIFICANCE
Butyric-d₇-acid is a cornerstone compound in modern metabolomic and isotopic labeling research. As a short-chain fatty acid derived from microbial fermentation, butyric acid plays vital roles in maintaining intestinal barrier integrity, modulating immune response, and serving as an energy source for colonocytes. By using its deuterated form, scientists can trace metabolic transformations, quantify flux rates, and differentiate endogenous from exogenous sources of butyrate with exceptional precision.

Moreover, Butyric-d₇-acid contributes to epigenetic regulation studies through its ability to mimic HDAC inhibitory effects, offering insights into chromatin remodeling, cellular differentiation, and cancer metabolism. Its use as a labeled biomarker in mass spectrometry-based assays enhances data accuracy and reproducibility, leading to more reliable interpretation of biological and environmental data.

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SUMMARY
Butyric-d₇-acid (CAS 73607-83-7) is a high-purity, fully deuterated analog of butyric acid designed for use as a stable isotope-labeled standard in mass spectrometric and biochemical studies. With ≥98 atom % deuterium enrichment, it enables precise metabolic tracing, quantitation, and isotopic discrimination in complex biological matrices.

At ResolveMass Laboratories Inc., Butyric-d₇-acid is manufactured under rigorous quality standards to ensure exceptional isotopic enrichment, chemical stability, and analytical consistency. Its versatility across metabolomics, clinical chemistry, and environmental applications makes it an essential reagent for researchers seeking to uncover the molecular mechanisms of short-chain fatty acid metabolism and its broader implications in health and disease.

 

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