Citric-2,2,4,4-d4 acid | CAS 147664-83-3

Citric-2,2,4,4-d4 Acid

CAS Number: 147664-83-3
Synonyms: Citric Acid-2,2,4,4-d4; Citric Acid-d4; Citric-d4 Acid; 1,1,3,3-Tetradeuterio-2-hydroxypropane-1,2,3-tricarboxylic acid
Molecular Formula: C₆H₄D₄O₇
Molecular Weight: 196.15 g/mol
Purity: ≥ 98% (chemical); ≥ 98 atom% D (isotopic enrichment)

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Product Overview

Citric-2,2,4,4-d4 Acid is a deuterium-labelled analogue of citric acid in which four hydrogen atoms at the 2,2,4,4 positions have been replaced with deuterium. This stable isotope substitution results in a compound that is chemically identical to citric acid in reactivity and behavior but distinct in mass, making it an ideal tool for analytical and research applications that rely on mass spectrometric differentiation.

Citric acid is a key intermediate in cellular energy metabolism and the tricarboxylic acid (TCA) cycle. Its deuterated form retains the same tricarboxylic structure while providing a molecular mass shift that allows researchers to trace, quantify, and distinguish isotopically labelled molecules in complex biological or chemical matrices. This property makes Citric-2,2,4,4-d4 Acid highly valuable in biochemical, pharmaceutical, and metabolomic research.

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Applications of Citric acid-d4 | CAS 147664-83-3

1. Internal Standard for Mass Spectrometry
Citric-2,2,4,4-d4 Acid is widely used as an internal standard for quantitative LC–MS and GC–MS analysis. Because it co-elutes and ionizes similarly to native citric acid, it provides accurate calibration and compensation for matrix effects, instrument drift, or sample preparation variability. This makes it ideal for precise quantification of citric acid and related metabolites in biological fluids, food samples, and pharmaceutical products.

2. Metabolic Tracing and Flux Analysis
In metabolic studies, this compound serves as a stable isotope tracer to monitor the dynamics of the TCA cycle and related biochemical pathways. Incorporation and transformation of the deuterated label can be followed in cell cultures or in vivo systems, helping researchers understand energy metabolism, oxidative stress, and disease-related metabolic alterations.

3. Analytical Method Development and Validation
Citric-2,2,4,4-d4 Acid is used as a reference standard for developing and validating analytical methods involving organic acid profiling. Laboratories use it to ensure method reproducibility, sensitivity, and linearity, particularly in high-precision quantitation workflows.

4. Pharmaceutical and Biotechnological Research
The compound is suitable for use in pharmacokinetic studies, bioanalytical assays, and formulation stability testing. Its stable isotopic labeling allows accurate tracking of metabolic intermediates and degradation products.

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Features and Benefits

• High isotopic purity ensures consistent and reliable deuterium incorporation, minimizing unlabeled interference.

• High chemical purity suitable for demanding analytical applications.

• Stable and non-radioactive – deuterium is a stable isotope of hydrogen, offering safety and long shelf life.

• Excellent solubility in polar solvents such as water, methanol, and DMSO.

• Well-characterized physical and spectral data – suitable for NMR, MS, and IR identification.

• Ideal for use as a calibration or reference compound in metabolomics, clinical chemistry, and biochemical assays.

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Handling

Citric-2,2,4,4-d4 Acid should be handled using standard laboratory safety precautions. Use appropriate personal protective equipment such as gloves, safety goggles, and a lab coat. Avoid inhalation of dust and contact with eyes or skin. The material may cause mild irritation upon direct contact.

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Summary

Citric-2,2,4,4-d4 Acid (CAS 147664-83-3) is a premium stable-isotope-labelled compound designed for high-precision analytical and biochemical applications. With excellent isotopic enrichment and chemical purity, it serves as an indispensable tool for laboratories engaged in metabolomics, isotope tracing, and quantitative analysis. Its performance and stability make it ideal for research and method development where accuracy, reproducibility, and traceability are essential.

At ResolveMass Laboratories Inc., we provide high-quality isotope-labelled standards that meet the exacting requirements of modern analytical science. Custom packaging, technical support, and Certificates of Analysis (CoA) are available upon request to support your research needs.