Deuterated Acetophenone-d8 | CAS 19547-00-3

Deuterated Acetophenone-d8 | CAS 19547-00-3 is a stable isotope-labeled analogue of acetophenone in which eight hydrogen atoms have been replaced with deuterium (²H). This isotopic substitution provides nearly identical chemical behavior to the non-labeled compound while offering a distinct mass difference that makes it highly valuable for analytical chemistry, quantitative mass spectrometry, pharmaceutical research, environmental analysis, and forensic investigations.

Stable isotope-labeled compounds such as Deuterated Acetophenone-d8 are widely employed as internal standards in LC-MS, LC-MS/MS, GC-MS, and other advanced analytical techniques. Because the labeled compound closely mimics the extraction, chromatographic retention, and ionization properties of native acetophenone, it enables highly accurate quantitative analysis while minimizing variability caused by sample preparation, matrix effects, and instrument performance.

At ResolveMass Laboratories Inc., isotope-labeled reference standards are supplied to support pharmaceutical development, analytical method development, validation studies, bioanalytical research, and quality control laboratories requiring reliable analytical materials.

Chemical Information

Product Name: Deuterated Acetophenone-d8

CAS Number: 19547-00-3

Molecular Formula: C8D8O

Molecular Weight: Approximately 128.18 g/mol

Synonyms:

• Acetophenone-d8
• Phenyl methyl ketone-d8
• Ethanone, phenyl-d8

Applications of Deuterated Acetophenone-d8

Internal Standard for Mass Spectrometry

One of the most important applications of Deuterated Acetophenone-d8 is its use as an internal standard during quantitative LC-MS/MS and GC-MS analysis. Since its physicochemical properties closely resemble those of native acetophenone, both compounds behave similarly throughout sample extraction, chromatographic separation, and ionization. The measurable mass difference allows analytical software to distinguish between the analyte and the internal standard, improving quantification accuracy.

Pharmaceutical Research

Acetophenone derivatives are widely encountered in pharmaceutical chemistry as intermediates, degradation products, and synthetic building blocks. Deuterated Acetophenone-d8 assists researchers in:

• Analytical method development
• Validation of quantitative assays
• Stability studies
• Forced degradation investigations
• Pharmaceutical impurity analysis
• Drug development research

Its isotopic labeling helps improve reproducibility while reducing analytical uncertainty.

Analytical Method Validation

Regulatory laboratories and pharmaceutical organizations frequently employ isotope-labeled reference materials during analytical method validation. Deuterated Acetophenone-d8 supports evaluation of critical analytical parameters including:

• Accuracy
• Precision
• Recovery
• Matrix effects
• Extraction efficiency
• Instrument reproducibility
• Quantitative robustness

These characteristics make it valuable throughout analytical method lifecycle management.

Environmental Analysis

Acetophenone may be encountered in environmental monitoring programs involving industrial emissions, wastewater, soil, and environmental contamination studies. Deuterated Acetophenone-d8 serves as an internal standard for quantitative environmental analysis, improving confidence in trace-level measurements while compensating for extraction losses and instrumental variability.

Forensic and Toxicological Research

Forensic laboratories utilize isotope-labeled compounds to improve identification and quantification of organic compounds within complex biological matrices. Deuterated Acetophenone-d8 supports:

• Forensic chemistry
• Toxicological investigations
• Chemical exposure assessment
• Laboratory method verification

Its use enhances analytical reliability across challenging sample types.

Benefits of Using Deuterated Acetophenone-d8

Stable isotope-labeled standards provide several important analytical advantages over external calibration alone. Deuterated Acetophenone-d8 offers:

• Excellent compatibility with LC-MS and GC-MS methods
• Improved quantitative accuracy
• Compensation for matrix effects
• Enhanced method reproducibility
• Better correction for sample preparation losses
• Reliable instrument performance monitoring
• Improved precision in trace-level analysis
• Simplified quantitative workflows
• Support for analytical method validation

These benefits make isotope-labeled standards an essential component of modern quantitative analytical laboratories.

Role in LC-MS and GC-MS Quantification

Modern analytical laboratories increasingly rely on isotope dilution techniques for highly accurate quantitative measurements. In isotope dilution mass spectrometry, Deuterated Acetophenone-d8 is added to the sample at a known concentration prior to extraction. Because both labeled and unlabeled compounds undergo nearly identical analytical processes, variations caused by extraction efficiency, ion suppression, or instrument drift are effectively compensated.

This approach significantly improves confidence in analytical results and supports robust quantitative workflows across pharmaceutical, environmental, forensic, and research laboratories.

Research and Development Applications

Researchers utilize Deuterated Acetophenone-d8 across numerous scientific disciplines, including:

• Pharmaceutical analytical development
• Chemical synthesis research
• Organic chemistry
• Medicinal chemistry
• Process chemistry
• Impurity profiling
• Stability-indicating method development
• Reference standard preparation
• Academic research
• Contract analytical testing

Its versatility makes it an important tool wherever accurate quantitative analysis of acetophenone is required.

Quality Considerations

Stable isotope-labeled reference materials should exhibit high isotopic incorporation and consistent analytical performance. Laboratories selecting Deuterated Acetophenone-d8 should evaluate characteristics such as isotopic enrichment, chemical identity, stability, and suitability for their intended analytical application. Proper storage and handling help preserve product integrity and support reproducible analytical results over time.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. provides high-quality isotope-labeled reference materials designed to support pharmaceutical, biotechnology, academic, environmental, and analytical laboratories. With extensive expertise in mass spectrometry, analytical chemistry, and pharmaceutical research, ResolveMass is committed to delivering reliable products that facilitate accurate quantitative analysis and method development.

Whether supporting pharmaceutical research, analytical method validation, environmental testing, or forensic investigations, ResolveMass Laboratories Inc. offers isotope-labeled compounds that help laboratories achieve precise, reproducible, and dependable analytical results.

Learn more about Deuterated Chemicals through,

• Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends
• Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 
• ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States.
• Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 
• How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 
• How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 
• Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 
• Custom Synthesis of Deuterated Chemicals