Deuterated Dimethyl Terephthalate-2,3,5,6-d4 | CAS 74079-01-9

Deuterated Dimethyl Terephthalate-2,3,5,6-d4 (CAS 74079-01-9) is a stable isotope-labeled analogue of dimethyl terephthalate in which four aromatic hydrogen atoms are replaced with deuterium (²H). This isotopically enriched compound is widely employed as an internal standard, analytical reference material, and research reagent for quantitative mass spectrometry, isotope dilution studies, and method validation. The deuterium substitution preserves the chemical behavior of the native molecule while providing a distinct mass shift that enables precise differentiation during LC-MS, GC-MS, and HRMS analyses.

Stable isotope-labeled compounds have become indispensable tools in modern pharmaceutical, chemical, environmental, and polymer research because they significantly improve analytical accuracy while minimizing matrix effects and instrument variability. Deuterated Dimethyl Terephthalate-2,3,5,6-d4 is particularly valuable wherever reliable quantification of dimethyl terephthalate or related aromatic ester compounds is required.

Chemical Information

• Product Name: Deuterated Dimethyl Terephthalate-2,3,5,6-d4
• CAS Number: 74079-01-9
• Molecular Formula: C10H6D4O4
• Molecular Weight: 198.21 g/mol
• Chemical Class: Stable isotope-labeled aromatic diester
• Isotopic Labeling: Four deuterium atoms incorporated on the aromatic ring (2,3,5,6 positions)
• Synonyms: Dimethyl terephthalate-d4, DMT-d4, Dimethyl p-phthalate-d4, Terephthalic Acid-d4 Dimethyl Ester

Applications

Deuterated Dimethyl Terephthalate-2,3,5,6-d4 is widely utilized across analytical laboratories, pharmaceutical development programs, academic research institutions, and quality control laboratories. Its isotopic purity and structural similarity to the unlabeled compound make it an ideal internal standard for highly accurate quantitative analysis.

Major applications include:

• LC-MS/MS quantitative analysis
• GC-MS method development
• High-resolution mass spectrometry (HRMS)
• Stable isotope dilution assays
• Pharmaceutical analytical method validation
• Polymer degradation studies
• PET and polyester raw material research
• Environmental monitoring of aromatic esters
• Reference standard preparation
• Analytical quality control

Because the labeled compound behaves almost identically to the native analyte during extraction, chromatographic separation, and ionization, it provides highly reproducible quantitative performance even in complex biological or environmental matrices.

Benefits of Using a Deuterated Internal Standard

Stable isotope-labeled standards offer significant analytical advantages over external calibration alone. Deuterated Dimethyl Terephthalate-2,3,5,6-d4 compensates for sample preparation losses, extraction variability, matrix suppression, and instrumental fluctuations that commonly affect quantitative LC-MS and GC-MS workflows.

Key benefits include:

• Improved quantitative accuracy
• Enhanced analytical precision
• Compensation for matrix effects
• Reduced run-to-run variability
• Better recovery correction
• Increased confidence in regulatory submissions
• Excellent reproducibility during method validation
• Reliable calibration for trace-level analysis

These characteristics make isotope-labeled standards essential components of regulated analytical methods developed under GLP, GMP, and ICH validation guidelines.

Pharmaceutical and Research Applications

Although Dimethyl Terephthalate itself is primarily associated with polymer chemistry and specialty chemical manufacturing, its isotope-labeled analogue is widely used in analytical laboratories supporting pharmaceutical development and chemical research.

Researchers employ this compound during:

• Drug impurity profiling
• Forced degradation studies
• Analytical method transfer
• Stability-indicating method development
• Metabolite identification workflows
• Process chemistry investigations
• Reference material qualification
• Instrument performance verification

Its predictable chromatographic behavior combined with a distinguishable mass difference allows simultaneous detection of both labeled and unlabeled compounds without altering chromatographic conditions.

Compatibility with Modern Analytical Techniques

Deuterated Dimethyl Terephthalate-2,3,5,6-d4 is compatible with numerous analytical platforms commonly used in research and regulated laboratories, including:

• Liquid Chromatography-Mass Spectrometry (LC-MS)
• Tandem LC-MS/MS
• Gas Chromatography-Mass Spectrometry (GC-MS)
• High-Resolution Mass Spectrometry (HRMS)
• Triple Quadrupole Mass Spectrometry
• Orbitrap Mass Spectrometry
• Time-of-Flight (TOF) Mass Spectrometry

Its excellent stability and isotopic integrity make it suitable for both routine quantitative assays and advanced research applications.

Quality and Handling

For reliable analytical performance, isotope-labeled reference standards should be manufactured under stringent quality control procedures. Typical characterization includes identity confirmation using NMR and mass spectrometry together with purity assessment using chromatographic techniques.

For long-term stability, the material should be stored according to the conditions specified on the Certificate of Analysis (COA) and Safety Data Sheet (SDS). Appropriate laboratory handling procedures should always be followed to maintain product integrity and analytical reproducibility.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. supplies high-quality stable isotope-labeled compounds designed to support pharmaceutical research, analytical method development, bioanalysis, forensic investigations, environmental testing, and advanced mass spectrometry applications. Our products are manufactured and characterized to meet the demanding requirements of research laboratories, CROs, pharmaceutical manufacturers, and academic institutions.

With a strong focus on analytical excellence, reliable documentation, and technical expertise, ResolveMass provides researchers with dependable isotope-labeled reference standards that improve quantitative accuracy, support regulatory compliance, and accelerate method development.

Whether you are developing validated LC-MS/MS methods, performing isotope dilution analysis, or conducting advanced analytical research, Deuterated Dimethyl Terephthalate-2,3,5,6-d4 (CAS 74079-01-9) offers the precision, consistency, and reliability required for high-quality scientific results.

Learn more about Deuterated Chemicals through,

• Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends
• Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 
• ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States.
• Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 
• How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 
• How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 
• Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 
• Custom Synthesis of Deuterated Chemicals