Deuterated Isobutyric-d7 Acid (CAS 223134-74-5)

Overview

Deuterated Isobutyric-d7 Acid (CAS 223134-74-5), also known as 2-Methylpropanoic-d7 Acid or Isobutyric Acid-d7, is a stable isotope-labeled analog of isobutyric acid in which all seven hydrogen atoms have been replaced by deuterium (²H). This complete deuterium substitution provides a distinct mass shift of +7 Da, making the compound an indispensable internal standard for high-precision quantitative analysis using gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS/MS), isotope dilution mass spectrometry (IDMS), and nuclear magnetic resonance (NMR). The compound retains the same chemical properties as native isobutyric acid while offering superior analytical discrimination due to its isotopic labeling.

Stable isotope-labeled standards such as Deuterated Isobutyric-d7 Acid are widely used in pharmaceutical research, metabolomics, food science, environmental analysis, and biochemical investigations where accurate quantification of volatile fatty acids (VFAs) is essential. Since the labeled compound behaves almost identically to the native analyte during extraction, chromatography, and ionization, it effectively compensates for sample preparation losses, matrix effects, and instrumental variability, thereby improving analytical accuracy and reproducibility.

Chemical Information

• Product Name: Deuterated Isobutyric-d7 Acid
• Synonyms: Isobutyric Acid-d7, 2-Methylpropanoic-d7 Acid
• CAS Number: 223134-74-5
• Molecular Formula: C₄D₇O₂
• Molecular Weight: 95.15 g/mol
• Mass Shift: M+7
• Chemical Class: Stable isotope-labeled branched-chain short-chain fatty acid
• Typical Isotopic Enrichment: ≥98 atom % D
• Appearance: Colorless liquid
• Research Use: Laboratory research and analytical applications only; not intended for human or veterinary use.

Applications:

Quantitative Mass Spectrometry

One of the primary applications of Deuterated Isobutyric-d7 Acid is as an internal standard in quantitative GC-MS and LC-MS/MS assays. The isotope-labeled compound co-elutes with native isobutyric acid while producing a distinguishable mass spectrum, enabling highly accurate quantification even in complex biological or environmental matrices.

Isotope Dilution Mass Spectrometry (IDMS)

IDMS is regarded as one of the most accurate quantitative analytical techniques available. Deuterated Isobutyric-d7 Acid serves as an ideal calibration standard because it compensates for analyte loss during extraction, derivatization, and sample handling while minimizing analytical bias.

Metabolomics Research

Short-chain fatty acids and branched-chain organic acids are important metabolic intermediates. Deuterated Isobutyric-d7 Acid is widely employed in metabolomics studies investigating branched-chain amino acid metabolism, gut microbiome activity, microbial fermentation, and cellular metabolic pathways.

Pharmaceutical Research

Pharmaceutical laboratories utilize isotope-labeled standards during bioanalytical method development and validation. Deuterated Isobutyric-d7 Acid supports assay validation by improving precision, recovery studies, accuracy, and quality control throughout drug development workflows.

Food and Fermentation Analysis

Isobutyric acid contributes to aroma profiles in fermented foods, dairy products, beverages, and flavor formulations. The deuterated analog enables precise measurement of naturally occurring isobutyric acid during quality control, fermentation monitoring, and flavor research.

Environmental Monitoring

Environmental laboratories use Deuterated Isobutyric-d7 Acid to quantify volatile fatty acids present in wastewater, soil, sediments, and industrial samples. The isotope-labeled compound improves confidence in trace-level detection and supports regulatory analytical methods.

Analytical Method Development

Researchers developing new chromatographic methods use isotope-labeled standards to optimize extraction procedures, evaluate matrix effects, establish calibration curves, determine recovery, and validate analytical performance according to regulatory expectations.

Advantages of Deuterated Isobutyric-d7 Acid

Deuterated Isobutyric-d7 Acid offers several important advantages over conventional reference materials:

• High isotopic enrichment provides excellent spectral separation from native isobutyric acid.
• Produces reliable quantitative results across GC-MS and LC-MS/MS platforms.
• Corrects for extraction variability and instrument fluctuations.
• Improves assay precision, reproducibility, and analytical confidence.
• Chemically equivalent to unlabeled isobutyric acid, ensuring identical chromatographic behavior.
• Suitable for trace-level quantitative analysis in complex matrices.
• Supports regulatory method validation and quality assurance programs.
• Compatible with metabolomics, pharmaceutical, food, and environmental analytical workflows.

Why Use Stable Isotope-Labeled Standards?

Stable isotope-labeled reference materials have become the gold standard for quantitative analytical chemistry. Unlike structurally different internal standards, deuterated analogs closely mimic the behavior of the target analyte throughout the analytical workflow. Because Deuterated Isobutyric-d7 Acid exhibits nearly identical extraction efficiency, chromatographic retention, and ionization characteristics as native isobutyric acid, it minimizes analytical variability while enabling accurate isotope dilution quantification.

This capability is particularly valuable for trace-level analysis, pharmacokinetic investigations, metabolomics research, food authenticity testing, and environmental monitoring where analytical precision is critical.

ResolveMass Laboratories Inc.

ResolveMass Laboratories Inc. supplies high-quality stable isotope-labeled compounds designed to support pharmaceutical development, analytical chemistry, metabolomics, environmental research, and life science applications. Our Deuterated Isobutyric-d7 Acid (CAS 223134-74-5) is manufactured to meet the demanding requirements of modern analytical laboratories, providing high isotopic enrichment, consistent quality, and dependable performance for quantitative mass spectrometry and isotope dilution analyses.

Whether your laboratory is developing validated analytical methods, conducting metabolomic investigations, performing environmental testing, or establishing quality control procedures, Deuterated Isobutyric-d7 Acid from ResolveMass Laboratories Inc. delivers the accuracy, reproducibility, and reliability required for advanced scientific research.

Learn more about Deuterated Chemicals through,

• Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends
• Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 
• ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States.
• Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 
• How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 
• How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 
• Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 
• Custom Synthesis of Deuterated Chemicals