Deuterated tert-Butanol-d9 | CAS 25725-11-5

Deuterated tert-Butanol-d9 | CAS 25725-11-5 is a stable isotope-labeled version of tert-butanol in which nine hydrogen atoms are replaced by deuterium (²H). This isotopically enriched alcohol is widely utilized in analytical chemistry, pharmaceutical research, metabolomics, environmental analysis, and mass spectrometry. The incorporation of deuterium increases the molecular mass by 9 Da (M+9) while maintaining nearly identical chemical behavior to the non-labeled compound, making it an ideal internal standard and research reagent.

At ResolveMass Laboratories Inc., we supply premium-quality deuterated reference standards that support accurate quantitative analysis, LC-MS/MS method development, GC-MS applications, isotope dilution studies, and pharmaceutical research. Our isotope-labeled compounds are manufactured to stringent quality standards, ensuring excellent isotopic enrichment, high chemical purity, and reliable analytical performance.

Product Overview

What is Deuterated tert-Butanol-d9?

Deuterated tert-Butanol-d9 is the fully deuterated analogue of tert-butanol. The replacement of hydrogen atoms with deuterium provides a measurable mass difference while preserving nearly identical physical and chemical characteristics. This unique property allows scientists to distinguish the labeled compound from its native counterpart using mass spectrometry without significantly altering chromatographic retention or chemical reactivity.

Because of these characteristics, tert-Butanol-d9 serves as an excellent isotopically labeled internal standard for quantitative analysis, method validation, recovery studies, and isotope tracing experiments.

Applications

LC-MS/MS Internal Standard

Deuterated tert-Butanol-d9 is commonly employed as an internal standard in liquid chromatography-mass spectrometry (LC-MS/MS). It compensates for sample preparation variability, extraction efficiency, matrix effects, and instrument response fluctuations, leading to improved quantitative accuracy.

GC-MS Method Development

Gas chromatography coupled with mass spectrometry (GC-MS) frequently utilizes tert-Butanol-d9 during analytical method development and validation. The isotopic mass shift enables easy differentiation between endogenous tert-butanol and the internal standard while maintaining similar chromatographic behavior.

Pharmaceutical Research

Stable isotope-labeled alcohols are valuable tools during pharmaceutical development. Researchers use tert-Butanol-d9 for:

• Drug impurity investigations
• Residual solvent analysis
• Method qualification
• Bioanalytical assay development
• Quality control testing

Metabolic and Isotope Tracing Studies

Scientists use deuterated compounds to investigate reaction mechanisms, metabolic pathways, molecular transformations, and compound fate within biological systems without introducing radioactive isotopes.

Analytical Method Validation

tert-Butanol-d9 supports:

• Precision studies
• Accuracy assessments
• Recovery experiments
• Stability testing
• Calibration curve preparation
• Regulatory analytical validation

Benefits of Using Deuterated tert-Butanol-d9

Researchers choose deuterated internal standards because they closely mimic the behavior of the native analyte while remaining distinguishable by mass spectrometry. Key advantages include:

• High isotopic enrichment
• Excellent analytical reproducibility
• Improved quantitative accuracy
• Reduced matrix interference
• Reliable correction for extraction losses
• Enhanced LC-MS and GC-MS performance
• Suitable for isotope dilution techniques
• Compatible with pharmaceutical quality systems

These benefits make tert-Butanol-d9 an essential reagent for laboratories requiring precise quantitative measurements.

Research Fields

Deuterated tert-Butanol-d9 supports research across numerous scientific disciplines, including:

• Pharmaceutical development
• Bioanalytical research
• Clinical research
• Environmental monitoring
• Toxicology
• Forensic science
• Chemical synthesis
• Stable isotope research
• Academic laboratories
• Contract research organizations (CROs)

Quality at ResolveMass Laboratories Inc.

ResolveMass Laboratories Inc. supplies isotope-labeled reference materials manufactured with strict quality control procedures to support demanding analytical workflows. Our stable isotope products are suitable for research laboratories, pharmaceutical companies, universities, government laboratories, and analytical testing facilities.

Every batch is produced with attention to:

• High chemical purity
• High isotopic enrichment
• Consistent batch-to-batch quality
• Reliable analytical performance
• Secure packaging
• Professional technical support

Our commitment to quality ensures dependable performance in advanced analytical applications where reproducibility and accuracy are essential.

Storage Recommendations

For optimal stability, Deuterated tert-Butanol-d9 should be stored in tightly sealed containers under recommended laboratory storage conditions. Protect the material from excessive moisture, heat, and contamination to preserve isotopic integrity and analytical performance. Many suppliers recommend refrigerated storage (2–8°C) for long-term stability, although specific handling should follow the product’s certificate and safety documentation.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. specializes in high-quality stable isotope-labeled compounds designed for advanced analytical laboratories. We understand the demanding requirements of pharmaceutical, biotechnology, environmental, and academic research, delivering products that support reliable analytical workflows.

Our customers benefit from:

• Premium isotope-labeled standards
• Competitive pricing
• Consistent product quality
• Reliable worldwide supply
• Technical expertise
• Fast customer support
• Custom synthesis capabilities for selected isotope-labeled compounds

Whether you are developing a new analytical method, validating an LC-MS/MS assay, performing isotope dilution mass spectrometry, or conducting pharmaceutical research, Deuterated tert-Butanol-d9 provides the accuracy and consistency required for dependable scientific results.

For Research Use Only. Not intended for human or veterinary use.

Learn more about Deuterated Chemicals through,

• Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends
• Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 
• ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States.
• Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 
• How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 
• How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 
• Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 
• Custom Synthesis of Deuterated Chemicals