Diethyl Phthalate-3,4,5,6-d4 | CAS 93952-12-6

OVERVIEW  of  Diethyl Phthalate-3,4,5,6-d4 | CAS 93952-12-6

Diethyl Phthalate-3,4,5,6-d₄ (DEP-d₄) is a deuterium-labeled phthalate ester engineered specifically for high-accuracy analytical quantification of diethyl phthalate in complex matrices. Diethyl phthalate itself is a widely used plasticizer and solvent found in personal care products, plastics, coatings, adhesives, and industrial formulations. Because of this broad presence, DEP frequently appears as a contaminant in environmental samples, consumer goods, indoor dust, water streams, and biological systems. DEP-d₄ provides a reliable isotope-labeled counterpart for analytical laboratories that require precise quantification, low-level detection, and robust calibration curves using isotope-dilution mass spectrometry.

The inclusion of four deuterium atoms located on the aromatic ring generates a clear mass difference relative to the native compound without altering its chromatographic or physicochemical behavior. This ensures near-identical extraction recovery, ionization efficiency, and retention characteristics—qualities essential for accurate internal standardization. For laboratories performing regulatory compliance, environmental monitoring, toxicology studies, or quality control testing, DEP-d₄ represents a highly stable, reproducible, and method-enhancing analytical reagent.

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CHEMICAL INFORMATION

• Name: Diethyl phthalate-d₄

• Molecular Formula: C₁₂H₁₀D₄O₄

• Molecular Weight: ~226.26 g/mol

• CAS Number: 93952-12-6

• Isotopic Enrichment: Typically ≥98% deuterium

• Chemical Classification: Deuterated aromatic diester (phthalate family)

• Stability: Highly stable under normal laboratory storage; resistant to hydrolysis under neutral conditions

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APPLICATIONS  of  Diethyl Phthalate-3,4,5,6-d4 | CAS 93952-12-6
Diethyl Phthalate-d₄ serves a wide range of analytical and research roles:

• Internal Standard for GC-MS and LC-MS:
  Used extensively in quantitative analysis of diethyl phthalate in plastics, resins, adhesives, consumer goods, and environmental samples. Its predictable mass shift ensures clean separation from native DEP in mass spectra.

• Environmental Contaminant Monitoring:
  Ideal for wastewater, surface water, groundwater, indoor dust, soil, and atmospheric particulate analysis. DEP-d₄ enhances accuracy in studies evaluating phthalate distribution and persistence.

• Consumer Product Quality Testing:
  Used to monitor phthalate levels in cosmetics, fragrances, packaging materials, and household items where phthalates may migrate or leach.

• Toxicology and Exposure Studies:
  Facilitates research into metabolic pathways, biological uptake, and exposure assessment of phthalates in human and animal studies by providing a stable isotopic reference point.

• Polymer and Material Science:
  Supports investigations into material migration, degradation, and polymer-additive interactions.

• Analytical Method Development:
  Essential for establishing calibration curves, extraction recovery studies, method validation, accuracy/precision assessments, and laboratory QC protocols.

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ADVANTAGES  of  Diethyl Phthalate-3,4,5,6-d4 | CAS 93952-12-6

• High Mass Spectral Clarity:
  The four deuterium atoms create a distinct mass separation from unlabeled diethyl phthalate, allowing exact quantification even in contaminated or complex matrices.

• Structural and Chemical Equivalence:
  DEP-d₄ behaves almost identically to native DEP in chromatographic and ionization processes, ensuring reliable internal standard performance.

• Enhanced Analytical Reliability:
  Improves measurement precision, signal reproducibility, and quantitative accuracy across a broad concentration range.

• Ideal for Trace-Level Analysis:
  Allows quantification of phthalates at very low levels, critical for regulatory testing and sensitive environmental surveillance.

• Stable and Non-Reactive:
  Maintains integrity under typical laboratory conditions and supports long-term method standardization.

• Versatile Across Platforms:
  Compatible with GC-MS, LC-MS, ESI-MS, APPI-MS, APCI-MS, and high-resolution mass spectrometry systems.

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SUMMARY
Diethyl Phthalate-3,4,5,6-d₄ is a high-performance, stable-isotope-labeled internal standard specifically designed to support accurate measurement of diethyl phthalate in environmental, biological, industrial, and consumer product samples. With four strategically placed deuterium atoms, DEP-d₄ provides superior mass differentiation without compromising chromatographic similarity. This makes it ideal for isotope-dilution quantification, method validation, and regulatory compliance workflows. Its stability, reproducibility, and compatibility with major analytical platforms position it as an essential tool for laboratories focusing on phthalate monitoring, contaminant tracing, material safety testing, and precision analytical chemistry.

 

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