DL-Alanine-d4 | CAS 53795-92-9

OVERVIEW
DL-Alanine-d₄ is a deuterium-labelled analogue of the naturally occurring amino acid DL-alanine, in which four hydrogen atoms are substituted with deuterium. This isotopic modification results in a compound that maintains the same physicochemical and biochemical characteristics as its non-deuterated counterpart but is distinguishable in spectroscopic and mass spectrometric analyses.

As a racemic mixture, DL-Alanine-d₄ contains equal proportions of the D- and L-enantiomers of alanine. The incorporation of deuterium atoms provides a unique mass shift (+4 Da), making this compound particularly valuable for isotope-dilution assays, tracer studies, and NMR applications. It is widely used in metabolic research, protein labelling, and the calibration of analytical instruments for amino acid quantification.

CHEMICAL INFORMATION

• Name: DL-Alanine-d₄

• Molecular Formula: C₃H₃D₄NO₂

• Molecular Weight: 93.12 g/mol

• CAS Number: 53795-92-9

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated amino acid

• Stability: Stable under ambient conditions; resistant to isotopic exchange under dry storage

APPLICATIONS  of  DL-Alanine-d4 | CAS 53795-92-9

• Isotope-Dilution Mass Spectrometry (IDMS):
  DL-Alanine-d₄ is a key internal standard for quantitative determination of alanine and related metabolites in biological fluids, tissue extracts, and fermentation broths. The +4 Da isotopic shift allows for accurate differentiation from native alanine peaks, improving quantification precision.

• NMR Spectroscopy:
  The deuterium substitution provides reduced proton signal interference in NMR spectra, allowing clearer spectral resolution. It is commonly used as a reference compound or solvent suppression standard in proton-decoupled NMR analyses.

• Metabolic Tracer Studies:
  As an isotopically labelled amino acid, DL-Alanine-d₄ is employed to study amino acid metabolism, protein synthesis, and nitrogen exchange pathways. Researchers use it to trace carbon and nitrogen flow in biochemical networks.

• Proteomics and Stable Isotope Labelling:
  In proteomic experiments, DL-Alanine-d₄ can be incorporated into proteins to provide labelled peptides for mass spectrometry-based quantitation, enabling precise comparative analysis of protein expression.

• Pharmaceutical and Biochemical Research:
  Used as a model compound in studies of amino acid racemization, enzyme catalysis, and metabolic flux analysis. Deuterium substitution also enables investigation of kinetic isotope effects (KIEs) in enzymatic transformations.

ADVANTAGES  of  DL-Alanine-d4 | CAS 53795-92-9

• High isotopic enrichment ensures excellent analytical precision and reproducibility.

• Chemically identical to natural alanine, preserving biological compatibility.

• Ideal internal standard for LC-MS and GC-MS analyses of amino acids.

• Enhanced signal clarity in NMR due to reduced proton resonance.

• Stable and easy to handle under normal laboratory conditions.

• Supports both fundamental biochemical research and applied analytical chemistry.

HANDLING
DL-Alanine-d₄ is a low-hazard compound and can be handled safely with standard laboratory practices.

• Avoid inhalation or contact with skin and eyes.

• Use protective gloves, lab coat, and eye protection.

• Store in airtight containers at room temperature or under refrigeration to minimize isotopic exchange.

• Keep away from strong oxidizing agents and moisture.

• In case of contact, rinse thoroughly with water and seek medical attention if irritation persists.

QUALITY & SPECIFICATION
Each batch of DL-Alanine-d₄ undergoes rigorous quality control testing to ensure chemical purity and isotopic enrichment. Analytical verification is typically performed using NMR spectroscopy, infrared spectroscopy (IR), and high-resolution mass spectrometry (HRMS).

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Residual Solvents: Below ICH limits

• Analytical Methods: Verified via ¹H NMR, ²H NMR, and MS profiles
  A Certificate of Analysis (COA) is provided for each lot, confirming compliance with analytical and isotopic specifications.

SUMMARY
DL-Alanine-d₄ (CAS 53795-92-9) is a deuterium-labelled analogue of DL-alanine used extensively in analytical chemistry, biochemistry, and isotopic tracer research. Its high isotopic purity and structural equivalence to natural alanine make it an indispensable standard in LC-MS, GC-MS, and NMR studies. With applications ranging from metabolic pathway analysis to stable isotope labelling in proteomics, DL-Alanine-d₄ represents a versatile and reliable reagent for modern scientific research, supporting precise quantitative and mechanistic investigations across multiple disciplines.