DL-Glutamic-2,4,4-d3 acid | CAS 96927-56-9

OVERVIEW  of  DL-Glutamic-2,4,4-d3 acid | CAS 96927-56-9

DL-Glutamic-2,4,4-d3 acid (CAS 96927-56-9) is a deuterium-labeled analog of DL-glutamic acid, incorporating three deuterium atoms at the 2 and 4 positions. As one of the core amino acids used in biochemical, metabolic, and isotopic tracing studies, the deuterated version enables highly accurate analysis in LC-MS, GC-MS, and NMR-based investigations. Its stable isotopic labeling provides a reliable mass shift without altering the native compound’s chemical behavior, making it ideal for metabolic flux analysis, protein turnover research, and bioanalytical quantitation.

At ResolveMass Laboratories Inc., DL-Glutamic-2,4,4-d3 acid is manufactured to high isotopic and chemical purity, ensuring reproducibility for both research and regulated analytical applications. This compound is widely used across metabolomics, stable isotope-resolved metabolomics (SIRM), nutritional biochemistry, neurochemical studies, and method development for amino acid profiling.

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CHEMICAL INFORMATION

• Chemical Name: DL-Glutamic-2,4,4-d3 acid

• Synonyms: Glutamic-d3 acid, 2,4,4-d3-DL-glutamic acid, Deuterated glutamate

• CAS Number: 96927-56-9

• Molecular Formula: C₅H₈D₃NO₄

• Molecular Weight: 150.15 g/mol

• Isotopic Enrichment: ≥98 atom % D

• Functional Groups: Amino acid (NH₂), two carboxylic acids (COOH)

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APPLICATIONS  of  DL-Glutamic-2,4,4-d3 acid | CAS 96927-56-9

• Metabolomics & SIRM: Serves as a tracer to study glutamate metabolism, TCA cycle flux, neurotransmitter pathways, and nitrogen cycling.

• Internal Standard for MS: Used as an internal standard for quantifying glutamic acid in plasma, serum, CSF, tissues, and food products using LC-MS/MS or GC-MS.

• Biochemical Research: Supports studies on oxidative stress, neural excitatory signaling, and amino acid biosynthesis.

• Nutritional Science: Helps evaluate amino acid utilization, dietary absorption, and metabolic turnover rates in organisms.

• Neurochemical Analysis: Ideal for research into glutamate’s role as the primary excitatory neurotransmitter in the central nervous system.

• Quality Control & Method Validation: Used in amino acid profiling, calibration curve preparation, and method validation under bioanalytical regulatory environments.

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FEATURES & BENEFITS

• High Isotopic Purity: Ensures clear mass spectral separation from native DL-glutamic acid.

• Structurally Identical Behavior: Retains the same chemical reactivity and chromatographic properties as the non-labeled amino acid.

• Versatile Use: Compatible with LC-MS, GC-MS derivatization workflows, and NMR-based metabolic mapping.

• Reliable for Research & Pharma: Meets the rigorous needs of bioanalytical and metabolic research laboratories.

• Available for Custom Synthesis: ResolveMass Laboratories provides tailored isotopologue synthesis to support specialized research pathways.

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SUMMARY
DL-Glutamic-2,4,4-d3 acid (CAS 96927-56-9) is a high-quality deuterated amino acid widely used in metabolic tracing, LC-MS/MS quantitation, and biochemical pathway analysis. Its three deuterium atoms offer predictable isotopic stability and improved analytical precision without altering the molecule’s natural biological interactions. Manufactured by ResolveMass Laboratories Inc., this compound supports a broad spectrum of research—from metabolomics to neurochemistry—ensuring consistent performance in both exploratory and regulated analytical environments.

 

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