Edoxaban-d6 HCl | CAS 912273-65-5

Edoxaban-d6 HCl is a stable isotope-labeled analogue of edoxaban, a direct oral Factor Xa inhibitor widely prescribed for the prevention and treatment of thromboembolic disorders. Incorporating six deuterium atoms (d6), Edoxaban-d6 HCl exhibits nearly identical physicochemical and chromatographic properties to the unlabeled drug while maintaining a distinguishable mass shift during mass spectrometric analysis. This unique characteristic makes it an ideal internal standard for LC-MS/MS quantification of edoxaban in biological matrices, pharmaceutical formulations, pharmacokinetic studies, therapeutic drug monitoring, and bioequivalence assessments. Stable isotope-labeled analogues such as Edoxaban-d6 are routinely used in isotope dilution mass spectrometry because they compensate for extraction variability, ion suppression, and instrument fluctuations, thereby improving analytical precision and accuracy.

At ResolveMass Laboratories Inc., we supply premium-quality isotope-labeled reference standards that support pharmaceutical development, regulated bioanalysis, forensic toxicology, clinical research, and method validation in accordance with international regulatory expectations.

Product Overview

• Product Name: Edoxaban-d6 HCl
• CAS Number: 912273-65-5
• Molecular Type: Stable Isotope-Labeled Reference Standard
• Labeling: Six Deuterium Atoms (d6)
• Salt Form: Hydrochloride

Why Use Edoxaban-d6 HCl?

Modern LC-MS/MS bioanalytical assays require highly reliable internal standards to ensure reproducible quantification across complex biological samples. Since Edoxaban-d6 HCl is chemically almost identical to the native analyte, it behaves similarly throughout sample preparation, chromatographic separation, and ionization. However, the isotopic mass difference allows the mass spectrometer to clearly distinguish it from endogenous edoxaban.

This significantly reduces analytical variability caused by:

• Matrix effects
• Sample preparation losses
• Ion suppression or ion enhancement
• Instrument drift
• Variable extraction efficiencies
• Injection-to-injection variability

Consequently, Edoxaban-d6 HCl is considered the preferred internal standard for quantitative LC-MS/MS methods used in regulated laboratories.

Applications:

LC-MS/MS Bioanalytical Method Development

Edoxaban-d6 HCl is extensively used during the development and validation of quantitative bioanalytical methods. It provides accurate normalization throughout the analytical workflow, enabling robust determination of edoxaban concentrations in plasma, serum, urine, and other biological matrices.

Pharmacokinetic (PK) Studies

During clinical pharmacokinetic studies, accurate measurement of plasma drug concentrations is essential for determining absorption, distribution, metabolism, and elimination profiles. Edoxaban-d6 HCl improves assay reproducibility and minimizes analytical bias.

Bioequivalence Studies

Generic drug manufacturers rely on isotope-labeled internal standards during bioequivalence studies submitted to regulatory agencies. The compound supports highly sensitive quantitative assays capable of measuring low plasma concentrations over extended sampling intervals.

Therapeutic Drug Monitoring

Although routine therapeutic monitoring of direct oral anticoagulants is not always required, measurement of edoxaban concentrations may be necessary in selected clinical situations including overdose, renal impairment, emergency surgery, or unexpected bleeding events. Stable isotope-labeled standards contribute to accurate laboratory quantification.

Clinical Research

Researchers investigating anticoagulant pharmacology, drug interactions, personalized medicine, and anticoagulant exposure frequently utilize Edoxaban-d6 HCl for validated quantitative assays.

Pharmaceutical Quality Control

Pharmaceutical laboratories employ isotope-labeled standards during analytical method validation, stability testing, impurity investigations, and assay verification to improve quantitative confidence.

Advantages of Stable Isotope-Labeled Standards

Compared with structural analogues, stable isotope-labeled internal standards provide superior analytical performance because they closely mimic the analyte throughout the complete analytical process.

Major benefits include:

• Excellent chromatographic co-elution
• Improved quantitative accuracy
• Enhanced precision
• Better correction for extraction losses
• Compensation for matrix effects
• Reduced analytical variability
• Greater reproducibility between analytical batches
• Compliance with regulatory bioanalytical guidelines

These characteristics make Edoxaban-d6 HCl particularly valuable in highly sensitive LC-MS/MS assays where accurate quantification at low concentrations is essential.

Typical Research Areas

Edoxaban-d6 HCl supports numerous scientific applications, including:

• Pharmacokinetic studies
• Pharmacodynamic investigations
• Bioavailability studies
• Bioequivalence studies
• Drug metabolism research
• Clinical toxicology
• Therapeutic drug monitoring
• Drug-drug interaction studies
• Forensic toxicology
• Method validation
• Analytical method transfer
• Biomarker research
• Pharmaceutical formulation studies
• Stability testing

Analytical Techniques

Edoxaban-d6 HCl is compatible with multiple advanced analytical platforms, including:

• LC-MS/MS
• UHPLC-MS/MS
• HPLC-MS/MS
• Triple Quadrupole Mass Spectrometry
• High-Resolution Mass Spectrometry (HRMS)
• Isotope Dilution Mass Spectrometry (IDMS)

Its excellent chromatographic behavior makes it suitable for both routine high-throughput laboratories and advanced pharmaceutical research facilities.

Pharmaceutical Development Support

As direct oral anticoagulants continue to play a significant role in cardiovascular medicine, pharmaceutical companies require reliable analytical standards throughout the drug development lifecycle. Edoxaban-d6 HCl supports:

• Discovery research
• Preclinical studies
• Clinical trials
• Regulatory submissions
• Generic drug development
• Contract research projects
• Quality assurance laboratories
• CRO analytical services

The use of isotope-labeled internal standards is widely recognized as a best practice for quantitative LC-MS/MS analysis because they closely track analyte behavior during extraction and detection while remaining distinguishable by mass spectrometry.

Quality and Research Confidence

ResolveMass Laboratories Inc. is committed to providing high-quality stable isotope-labeled reference standards that meet the needs of pharmaceutical, biotechnology, academic, and clinical research laboratories. Every product is intended to support reliable analytical performance, helping scientists achieve reproducible and regulatory-compliant results across diverse applications.

Whether supporting bioanalytical assay development, pharmacokinetic investigations, therapeutic drug monitoring, or pharmaceutical quality testing, Edoxaban-d6 HCl serves as a dependable internal standard for highly accurate LC-MS/MS quantification.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. delivers premium isotope-labeled standards backed by scientific expertise and a commitment to analytical excellence. Our products are designed to support:

• Pharmaceutical R&D
• Contract Research Organizations (CROs)
• Clinical laboratories
• Bioanalytical laboratories
• Academic research institutions
• Generic pharmaceutical manufacturers
• Regulatory compliance studies

With a focus on quality, consistency, and customer support, ResolveMass helps researchers generate reliable analytical data for drug development and life science research.

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