Ethylbenzene-d10 | CAS 25837-05-2

OVERVIEW  of  Ethylbenzene-d10 | CAS 25837-05-2

Ethylbenzene-d10 is the deuterated analog of ethylbenzene, an aromatic hydrocarbon that serves as a key intermediate in the production of styrene and other industrial chemicals. The complete substitution of hydrogen atoms with deuterium increases the molecular weight by +10 Da without altering the compound’s volatility, polarity, or chromatographic behavior.

At ResolveMass Laboratories Inc., Ethylbenzene-d10 is synthesized to achieve ≥99 atom % deuterium enrichment and ≥99% chemical purity, making it an ideal internal standard for the quantitative determination of ethylbenzene, xylene isomers, and other volatile organic compounds (VOCs) in complex matrices. Its high isotopic purity and chemical equivalence ensure consistent analytical performance in environmental, industrial, and forensic testing laboratories.

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SUMMARY
Ethylbenzene-d10 (CAS 25837-05-2) is a fully deuterated aromatic hydrocarbon in which all ten hydrogen atoms of ethylbenzene are replaced by deuterium. This stable isotope-labeled compound is widely used as an internal standard, calibration reference, and tracer in gas chromatography–mass spectrometry (GC-MS), environmental monitoring, and petrochemical analysis. Offered by ResolveMass Laboratories Inc., Ethylbenzene-d10 provides exceptional isotopic enrichment, chemical stability, and analytical reliability, ensuring precise quantification and reproducibility in advanced analytical and research applications.

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CHEMICAL INFORMATION

• Chemical Name: Ethylbenzene-d10

• Synonyms: Fully deuterated ethylbenzene; (C₆D₅)(CD₂CD₃)

• CAS Number: 25837-05-2

• Molecular Formula: C₈D₁₀

• Molecular Weight: 116.23 g/mol

• Isotopic Enrichment: ≥ 99 atom % D

• Chemical Structure: Benzene ring substituted with a deuterated ethyl group

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DESCRIPTION AND PROPERTIES
Ethylbenzene-d10 is a nonpolar, aromatic compound with physicochemical properties nearly identical to those of its non-deuterated analog. The replacement of hydrogen with deuterium atoms results in a predictable isotopic mass shift, making it an essential tool for mass spectrometry-based quantitative analyses.

This deuterated hydrocarbon provides stable, reproducible retention and ionization behavior, making it especially valuable as an internal standard for hydrocarbon analysis by GC-MS. It is also utilized in kinetic isotope effect studies, tracer experiments, and calibration of environmental monitoring systems for volatile organic compounds.

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APPLICATIONS  of  Ethylbenzene-d10 | CAS 25837-05-2

1. Internal Standard for GC-MS Analysis:
   Ethylbenzene-d10 is a preferred internal standard in gas chromatography–mass spectrometry for the quantification of ethylbenzene, xylene isomers, and other aromatic hydrocarbons in air, soil, and water samples. The distinct +10 Da mass shift allows precise isotope dilution quantification.

2. Environmental and Petrochemical Analysis:
   It is extensively used in environmental testing laboratories for the detection of VOCs under EPA and ISO protocols, as well as in petroleum and petrochemical industries for process monitoring and product quality assessment.

3. Isotope Dilution Mass Spectrometry (IDMS):
   As a high-accuracy isotopic reference material, Ethylbenzene-d10 enables robust quantification in complex matrices by compensating for matrix effects and instrumental variations.

4. Tracer and Mechanistic Studies:
   The compound serves as a non-radioactive tracer in studying hydrocarbon transport, biodegradation, and environmental fate, offering safe and stable labeling for experimental systems.

5. Kinetic Isotope Effect Studies:
   Deuterated hydrocarbons like Ethylbenzene-d10 are used to investigate reaction mechanisms and rate-determining steps in catalysis, oxidation, and hydrogen exchange reactions.

6. Calibration and Method Validation:
   Ethylbenzene-d10 provides a consistent reference standard for instrument calibration and performance verification in analytical laboratories, ensuring reproducibility and traceability of results.

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ANALYTICAL ADVANTAGES

• Fully deuterated structure provides a distinct mass shift (+10 Da)

• Excellent chemical and isotopic stability under analytical conditions

• Ideal for use as an internal standard in GC-MS and IDMS applications

• Chemically identical retention and ionization behavior to ethylbenzene

• Highly pure and non-reactive, minimizing background interference

• Complies with regulatory requirements for trace-level hydrocarbon detection

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QUALITY AND MANUFACTURING
At ResolveMass Laboratories Inc., Ethylbenzene-d10 is synthesized through advanced deuterium exchange and catalytic methods to ensure full isotopic labeling and maximum purity. Each batch undergoes comprehensive characterization using GC-MS, NMR spectroscopy, and IR analysis to confirm isotopic enrichment, chemical identity, and chromatographic performance.

Our manufacturing and quality control protocols conform to international analytical standards, providing customers with reliable, reproducible, and traceable deuterated reference materials. Whether used for routine testing, research, or regulatory compliance, Ethylbenzene-d10 from ResolveMass Laboratories ensures uncompromised analytical accuracy and consistency.

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CONCLUSION
Ethylbenzene-d10 is a high-quality, fully deuterated aromatic compound engineered for analytical precision in mass spectrometry, environmental monitoring, and chemical research. Its isotopic stability, chemical equivalence, and traceable purity make it an indispensable internal standard and calibration reference. Supported by ResolveMass Laboratories Inc., this compound offers scientists and analytical professionals a reliable, reproducible, and high-performance isotope-labeled reagent for advanced GC-MS and environmental testing workflows.

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