Glipizide-d11 | CAS 29094-61-9

Glipizide-d11 is a stable isotope-labeled analog of glipizide in which eleven hydrogen atoms are replaced with deuterium (²H). This isotopically labeled compound is widely used as an internal standard (IS) for the accurate quantification of glipizide in biological matrices using LC-MS/MS and other mass spectrometry-based analytical techniques. The incorporation of deuterium ensures that Glipizide-d11 exhibits nearly identical physicochemical properties to native glipizide while remaining distinguishable by its characteristic mass difference during mass spectrometric detection.

At ResolveMass Laboratories Inc., we recognize the critical role of stable isotope-labeled reference standards in regulated pharmaceutical research, bioanalytical method development, pharmacokinetic studies, therapeutic drug monitoring, and clinical research. Glipizide-d11 serves as a reliable internal standard that improves analytical accuracy, compensates for sample preparation variability, minimizes matrix effects, and enhances assay reproducibility.

Product Overview

• Product Name: Glipizide-d11
• CAS Number: 29094-61-9
• Molecular Formula: C₂₁H₂₇N₅O₄S
• Molecular Weight: 456.6 g/mol (isotope-labeled form)
• Isotopic Label: Deuterium (d11)
• Application: Stable isotope-labeled internal standard for quantitative LC-MS/MS analysis
• Research Use: For laboratory research and analytical applications only. Not intended for human or veterinary use.

Why Use Glipizide-d11?

Reliable quantitative bioanalysis requires an internal standard that closely mimics the behavior of the analyte throughout extraction, chromatographic separation, and ionization. Because Glipizide-d11 possesses nearly identical chemical properties to unlabeled glipizide, it co-extracts and co-elutes with the target analyte while producing a unique mass transition during MS detection.

This enables analysts to compensate for:

• Matrix suppression or enhancement
• Variability during sample extraction
• Instrument response fluctuations
• Sample handling losses
• Day-to-day analytical variability

The result is improved precision, accuracy, and robustness of validated bioanalytical methods.

Applications:

Glipizide-d11 is extensively utilized across pharmaceutical, biotechnology, CRO, and academic laboratories for numerous analytical applications.

LC-MS/MS Bioanalysis

The most common application of Glipizide-d11 is as an internal standard during quantitative LC-MS/MS assays for glipizide determination in plasma, serum, urine, and other biological matrices. Stable isotope dilution techniques significantly improve quantitative performance by correcting for extraction recovery and matrix effects.

Pharmacokinetic (PK) Studies

During preclinical and clinical pharmacokinetic studies, accurate plasma concentration measurements are essential for determining absorption, distribution, metabolism, and elimination characteristics. Glipizide-d11 supports high-quality PK data by providing consistent analytical normalization.

Bioequivalence (BE) Studies

Generic drug development requires validated analytical methods capable of demonstrating bioequivalence between test and reference products. Glipizide-d11 serves as an ideal internal standard for regulatory bioanalysis supporting ANDA submissions.

Therapeutic Drug Monitoring

Clinical laboratories developing highly sensitive analytical methods for therapeutic drug monitoring may utilize isotope-labeled internal standards to improve assay precision and reproducibility.

Method Development and Validation

Analytical scientists use Glipizide-d11 during method development to establish calibration models, evaluate extraction efficiency, assess matrix effects, determine recovery, and validate analytical performance according to regulatory guidelines.

Pharmaceutical Research

Research laboratories investigating sulfonylurea compounds, drug metabolism, formulation performance, or analytical characterization frequently employ stable isotope-labeled standards to generate high-quality quantitative data.

Advantages of Stable Isotope-Labeled Internal Standards

Compared with structurally unrelated internal standards, Glipizide-d11 offers several analytical advantages:

• Nearly identical chromatographic behavior to native glipizide
• Comparable extraction recovery
• Minimal chromatographic interference
• Improved quantitative accuracy
• Enhanced method robustness
• Better correction for matrix effects
• Excellent reproducibility across analytical batches
• Regulatory acceptance for validated LC-MS/MS methods

These characteristics make isotope dilution mass spectrometry one of the preferred quantitative analytical approaches in regulated pharmaceutical laboratories.

Role in Bioanalytical Method Validation

During bioanalytical method validation, Glipizide-d11 contributes to the evaluation of several critical performance characteristics, including:

• Accuracy
• Precision
• Selectivity
• Sensitivity
• Recovery
• Matrix effect assessment
• Stability studies
• Dilution integrity
• Calibration curve performance
• Quality control sample analysis

Use of an appropriate isotope-labeled internal standard helps laboratories meet regulatory expectations for reliable quantitative bioanalysis.

Quality Considerations

High-quality isotope-labeled reference standards are essential for generating dependable analytical results. Laboratories typically evaluate:

• Isotopic enrichment
• Chemical purity
• Identity confirmation
• Moisture content
• Storage stability
• Certificate of Analysis (CoA)
• Batch traceability

These quality attributes contribute to consistent analytical performance throughout method development and routine sample analysis.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. combines scientific expertise with state-of-the-art analytical technologies to support pharmaceutical research from early development through regulatory submission. Our team has extensive experience in mass spectrometry, analytical method development, bioanalysis, and reference standard characterization. We are committed to delivering reliable analytical solutions that meet the highest quality standards while supporting the evolving needs of pharmaceutical, biotechnology, and academic research organizations.

Whether your project involves pharmacokinetic studies, bioequivalence testing, bioanalytical method validation, or quantitative LC-MS/MS assay development, ResolveMass Laboratories Inc. provides dependable analytical support backed by technical excellence and regulatory expertise.

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