Glycerol-d8 | CAS 7325-17-9

OVERVIEW  of  Glycerol-d8 | CAS 7325-17-9

Glycerol-d8 (CAS 7325-17-9) is a fully deuterated analog of glycerol, where all hydrogen atoms are replaced by deuterium. This compound serves as a stable isotope-labeled reagent ideal for use in NMR spectroscopy, mass spectrometry, tracer studies, and kinetic isotope effect investigations. Owing to its complete deuterium substitution, Glycerol-d8 provides high isotopic purity, minimized proton interference in NMR, and enhanced analytical accuracy in isotopic quantification studies.

CHEMICAL INFORMATION

• Name: Glycerol-d8

• Molecular Formula: C₃D₈O₃

• Molecular Weight: 100.14 g/mol

• CAS Number: 7325-17-9

• Isotopic Labeling: Fully deuterated (eight deuterium atoms)

• Chemical Class: Deuterated polyol; stable isotope-labeled compound

• Stability: Chemically stable under ambient conditions

APPLICATIONS  of  Glycerol-d8 | CAS 7325-17-9

• NMR Spectroscopy:
  Used as a deuterated solvent or internal standard for hydrogen-exchange and relaxation studies. Its deuterium labeling minimizes proton signals, improving spectrum clarity in ¹H and ²H NMR.

• Mass Spectrometry (MS):
  Functions as a stable isotope internal standard in quantitative determination of glycerol and related metabolites.

• Tracer and Metabolic Studies:
  Applied in metabolomics and biochemical pathway tracing to monitor glycerol utilization and lipid biosynthesis.

• Kinetic Isotope Effect (KIE) Research:
  Used to evaluate isotope effects on hydrogen transfer, oxidation-reduction, and enzymatic reaction mechanisms.

• Calibration and Standardization:
  Serves as a reference standard for isotope-ratio calibration in analytical chemistry.

ADVANTAGES  of  Glycerol-d8 | CAS 7325-17-9

• Fully deuterated structure ensures high isotopic purity (≥ 98 atom % D).

• Minimizes proton background in NMR experiments for cleaner spectra.

• Chemically identical to native glycerol, enabling direct comparative studies.

• Excellent stability and reproducibility for isotope-labeled experiments.

• Non-radioactive and safe for routine laboratory use.

HANDLING, STORAGE & SAFETY

• Handling: Use under dry, inert conditions to avoid hydrogen–deuterium exchange.

• Storage: Keep tightly sealed at 2–8 °C; protect from light and humidity.

• Safety: Low toxicity; may cause mild irritation to skin or eyes.

• Disposal: Dispose of according to institutional chemical waste guidelines.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Physical State: Viscous liquid

• Analytical Validation: Verified by ¹H NMR, ²H NMR, and IR spectroscopy

• Certificate of Analysis (CoA): Supplied with each batch for isotopic verification

SUMMARY
Glycerol-d8 (CAS 7325-17-9) is a fully deuterium-labeled isotopologue of glycerol designed for high-precision analytical, biochemical, and isotopic research. Its complete deuteration ensures superior NMR signal suppression and accurate mass spectrometric quantification, making it indispensable in metabolomic, tracer, and mechanistic studies. With its high isotopic purity and chemical stability, Glycerol-d8 is a reliable reagent for advanced isotopic and spectroscopic applications.