Hexanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 2483831-95-2

OVERVIEW  of  Hexanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 2483831-95-2
Hexanoyl-L-Carnitine-d₃ HCl (N-methyl-d₃) is a stable isotopically labeled derivative of hexanoyl-L-carnitine, a medium-chain acylcarnitine that facilitates the transport of fatty acids into mitochondria for β-oxidation and energy production. In this labeled version, the three hydrogen atoms on the N-methyl group of the L-carnitine moiety are replaced with deuterium atoms, resulting in a +3 Da molecular mass shift. This isotopic substitution preserves the physicochemical and biological properties of the native compound, making it ideal as an internal standard for quantitative LC-MS/MS analysis.

It is commonly applied in targeted metabolomics, lipidomics, and clinical diagnostics to quantify endogenous acylcarnitines in biological matrices such as plasma, serum, and urine, ensuring accuracy and reproducibility in analytical assays.

CHEMICAL INFORMATION

• Name: Hexanoyl-L-Carnitine-d₃ hydrochloride

• Molecular Formula: C₁₃H₂₃D₃NO₄·HCl

• Molecular Weight: 298.82 g/mol

• CAS Number: 2483831-95-2

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated acylcarnitine; stable isotope-labeled metabolite

• Stability: Chemically stable under inert and dry conditions; sensitive to moisture

APPLICATIONS  of  Hexanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 2483831-95-2

• Targeted Metabolomics & Lipidomics:
  Used as a stable isotope-labeled internal standard for precise quantification of hexanoyl-L-carnitine and related acylcarnitines in biological matrices using LC-MS/MS.

• Clinical & Diagnostic Research:
  Supports the quantitative analysis of acylcarnitine profiles in plasma and urine for detecting fatty acid oxidation disorders (FAODs) and mitochondrial dysfunctions.

• Biochemical & Energy Metabolism Studies:
  Assists in elucidating fatty acid β-oxidation pathways and mitochondrial transport processes in metabolic research.

• Mass Spectrometry Calibration:
  Functions as a reference compound for retention time correction, instrument calibration, and response factor normalization in LC-MS assays.

• Quality Control & Standardization:
  Employed as a QC standard in high-throughput metabolomics workflows to enhance reproducibility and inter-laboratory consistency.

ADVANTAGES  of  Hexanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 2483831-95-2

• High isotopic enrichment (≥ 98 atom % D) enables clear differentiation from native molecules.

• Identical chromatographic and ionization characteristics to unlabeled hexanoyl-L-carnitine ensure accurate quantitation.

• Non-radioactive, stable, and safe for laboratory use.

• Improves analytical precision and accuracy in complex biological matrices.

HANDLING, STORAGE & SAFETY

• Handling: Handle in accordance with standard laboratory safety practices. Avoid inhalation, ingestion, and prolonged contact.

• Storage: Store at –20 °C under dry, inert conditions. Protect from light and moisture.

• Safety: Non-toxic and non-hazardous in analytical applications.

• Disposal: Dispose of waste materials according to institutional and local environmental regulations.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Form: Crystalline or amorphous powder

• Analytical Verification: Confirmed by LC-MS/MS and NMR spectroscopy

• Documentation: Supplied with Certificate of Analysis (CoA) verifying isotopic enrichment and purity

SUMMARY
Hexanoyl-L-Carnitine-d₃ HCl (N-methyl-d₃) | CAS 2483831-95-2 is a deuterium-labeled internal standard widely used in metabolomic and biochemical studies involving fatty acid metabolism. Its structural and chromatographic equivalence to the native compound, combined with high isotopic enrichment, ensures accurate, reproducible quantitation in LC-MS/MS analyses. This compound is particularly valuable in clinical diagnostics, energy metabolism research, and biochemical assay development, where precise detection of medium-chain acylcarnitines is essential.