L-Carnitine-d3 HCl (methyl-d3) | CAS 350818-62-1

OVERVIEW  of  L-Carnitine-d3 HCl (methyl-d3) | CAS 350818-62-1
L-Carnitine-d₃ HCl (methyl-d₃) is a stable isotope-labeled analog of naturally occurring L-carnitine, a vital quaternary ammonium compound that facilitates the transport of long-chain fatty acids into mitochondria for β-oxidation and energy production. In this labeled form, three hydrogens on the N-methyl group are replaced by deuterium atoms, yielding a +3 Da mass difference while preserving the same chemical and biochemical characteristics as the unlabeled compound.

This deuterated form serves as a precise internal standard for LC-MS/MS and GC-MS-based analyses of carnitine metabolism and acylcarnitine profiling. It enables accurate quantification and metabolic tracing of carnitine-related pathways in clinical diagnostics, nutritional biochemistry, and metabolomics research.

CHEMICAL INFORMATION

• Name: L-Carnitine-d₃ hydrochloride

• Molecular Formula: C₇H₁₁D₃NO₃·HCl

• Molecular Weight: 200.68 g/mol

• CAS Number: 350818-62-1

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated quaternary ammonium compound; stable isotope-labeled metabolite

• Stability: Stable under dry, inert, and low-temperature conditions

APPLICATIONS  of  L-Carnitine-d3 HCl (methyl-d3) | CAS 350818-62-1

• Metabolomics and Lipidomics:
  Utilized as a stable isotope-labeled internal standard for the quantification of L-carnitine and acylcarnitines in plasma, serum, urine, and tissue extracts via LC-MS/MS.

• Clinical and Nutritional Research:
  Supports the analysis of carnitine deficiency, mitochondrial dysfunction, and fatty acid oxidation disorders. It also aids in nutritional studies assessing energy metabolism and supplementation effects.

• Biochemical Pathway Studies:
  Applied in tracing the carnitine shuttle mechanism, which is essential for transporting fatty acids into mitochondria for ATP production.

• Mass Spectrometry Calibration:
  Serves as a quantitative reference for instrument calibration, peak identification, and isotopic correction in MS-based metabolomic workflows.

• Pharmaceutical and Toxicological Studies:
  Used in drug metabolism and toxicity investigations to monitor carnitine-dependent transport and detoxification pathways.

ADVANTAGES  of  L-Carnitine-d3 HCl (methyl-d3) | CAS 350818-62-1

• High isotopic enrichment (≥ 98 atom % D) for reliable isotopic distinction from unlabeled L-carnitine.

• Identical chromatographic and ionization properties to the non-deuterated analog ensure analytical accuracy.

• Non-radioactive, chemically stable, and safe to handle in standard laboratory environments.

• Enhances quantitative precision and data reproducibility in targeted metabolomic assays.

HANDLING, STORAGE & SAFETY

• Handling Precautions: Avoid moisture and prolonged air exposure. Handle under dry, inert conditions using appropriate protective equipment.

• Storage: Store tightly sealed at –20 °C in a dry environment, away from heat and light.

• Safety: Non-toxic and non-hazardous under standard laboratory handling procedures.

• Disposal: Dispose of following local regulations for organic laboratory chemicals.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Form: Crystalline or lyophilized powder

• Analytical Verification: Verified by LC-MS and NMR spectroscopy

• Documentation: Supplied with Certificate of Analysis (CoA) detailing isotopic enrichment and purity profile

SUMMARY
L-Carnitine-d₃ HCl (methyl-d₃) | CAS 350818-62-1 is a deuterium-labeled analog of L-carnitine, designed for quantitative metabolomic analysis and biochemical research. Its isotopic labeling on the N-methyl group makes it a robust internal standard for LC-MS/MS quantification of endogenous L-carnitine and acylcarnitine species. With exceptional isotopic purity and analytical stability, it plays a key role in studies of fatty acid oxidation, mitochondrial bioenerget

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