Lenvatinib-15N-d4 | CAS 417716-92-8

Lenvatinib-15N-d4 is a high-quality stable isotope-labeled analogue of lenvatinib designed for advanced bioanalytical, pharmacokinetic, metabolism, and pharmaceutical research applications. This isotopically labeled reference standard incorporates one nitrogen-15 (¹⁵N) atom and four deuterium (d4) atoms, making it an ideal internal standard for highly accurate quantification of lenvatinib using LC-MS/MS and high-resolution mass spectrometry (HRMS). Stable isotope-labeled standards are widely used in regulated bioanalytical laboratories because they closely mimic the physicochemical behavior of the native compound while remaining distinguishable by mass spectrometry.

Lenvatinib is a multi-targeted receptor tyrosine kinase (RTK) inhibitor that acts against several clinically important signaling pathways, including VEGFR1-3, FGFR1-4, PDGFRα, KIT, and RET. It is approved for the treatment of several cancers, including differentiated thyroid cancer, hepatocellular carcinoma, renal cell carcinoma, and endometrial carcinoma. Because of its increasing clinical use, there is significant demand for sensitive and reproducible analytical methods capable of accurately measuring lenvatinib concentrations in biological matrices and pharmaceutical formulations. Stable isotope-labeled compounds such as Lenvatinib-15N-d4 provide the highest level of analytical accuracy for these applications.

Why Use Lenvatinib-15N-d4?

Stable isotope-labeled internal standards are considered the gold standard for quantitative mass spectrometry. Lenvatinib-15N-d4 behaves nearly identically to unlabeled lenvatinib during sample preparation, chromatographic separation, ionization, and detection while exhibiting a predictable mass shift that enables precise differentiation by LC-MS/MS.

The use of Lenvatinib-15N-d4 helps compensate for:

• Matrix effects during electrospray ionization
• Sample preparation variability
• Instrument response fluctuations
• Extraction losses
• Day-to-day analytical variation

These characteristics significantly improve assay precision, reproducibility, and quantitative reliability, particularly in regulated bioanalytical laboratories operating under FDA, EMA, or ICH validation guidelines.

Applications

Lenvatinib-15N-d4 is suitable for a broad range of pharmaceutical and biomedical research applications, including:

• LC-MS/MS quantitative bioanalysis
• Pharmacokinetic (PK) studies
• Toxicokinetic studies
• Drug metabolism investigations
• ADME studies
• Therapeutic drug monitoring method development
• Bioequivalence studies
• Clinical research
• Method validation
• Pharmaceutical quality control
• Reference standard development
• High-resolution mass spectrometry workflows

Because the labeled analogue closely tracks the behavior of the analyte throughout the analytical process, it improves method robustness while minimizing analytical bias.

Benefits of Stable Isotope-Labeled Standards

Compared with structurally similar analogues, isotope-labeled internal standards offer several analytical advantages:

• Superior quantitative accuracy
• Improved precision across multiple analytical batches
• Compensation for extraction efficiency differences
• Reduced matrix interference
• Enhanced assay reproducibility
• Excellent compatibility with isotope dilution mass spectrometry (IDMS)
• Reliable quantification at low concentration levels
• Support for validated bioanalytical methods

These advantages make Lenvatinib-15N-d4 particularly valuable for CROs, pharmaceutical companies, academic researchers, and regulatory laboratories performing quantitative LC-MS/MS analysis.

Typical Research Areas

Researchers utilize Lenvatinib-15N-d4 in numerous scientific disciplines, including:

• Oncology drug development
• Clinical pharmacology
• Precision medicine research
• Biomarker studies
• Drug-drug interaction studies
• Pharmaceutical formulation development
• Biosample analysis
• Translational medicine
• Regulatory bioanalysis
• Mass spectrometry method optimization

The compound is especially useful during preclinical and clinical development where accurate quantification of lenvatinib is essential for pharmacokinetic modeling and exposure-response evaluation.

Analytical Compatibility

Lenvatinib-15N-d4 is compatible with numerous analytical platforms, including:

• LC-MS/MS
• UHPLC-MS/MS
• LC-HRMS
• Triple Quadrupole Mass Spectrometry
• QTOF Mass Spectrometry
• Orbitrap Mass Spectrometry
• Stable Isotope Dilution Assays

Its isotopic labeling allows co-elution with the native analyte while maintaining clear mass discrimination, providing excellent quantitative performance across a wide concentration range.

Quality Considerations

High-quality isotope-labeled reference standards are critical for generating reproducible analytical data. Researchers typically select standards that provide:

• High chemical purity
• High isotopic enrichment
• Comprehensive analytical characterization
• Batch-to-batch consistency
• Reliable long-term stability
• Suitable documentation for research and regulated laboratory use

These attributes contribute to robust analytical methods and dependable quantitative results.

Technical Information

Product Name: Lenvatinib-15N-d4

CAS (Unlabeled): 417716-92-8

Molecular Formula: C21H15D4ClN3¹⁵NO4

Molecular Weight: Approximately 431.87 g/mol

Category: Stable Isotope-Labeled Standard

Applications: LC-MS/MS, HRMS, pharmacokinetics, bioanalysis, drug metabolism, therapeutic drug monitoring, pharmaceutical research, analytical method validation, isotope dilution mass spectrometry.

Research Use Only

Lenvatinib-15N-d4 is supplied exclusively for research and laboratory use. It is intended as an analytical reference material and internal standard for scientific investigations. It is not intended for human or veterinary use, therapeutic applications, or diagnostic procedures.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. provides high-quality stable isotope-labeled reference standards and analytical support for pharmaceutical, biotechnology, and academic research organizations. Our expertise in mass spectrometry, bioanalysis, pharmaceutical characterization, and analytical method development enables researchers to achieve reliable, reproducible, and regulatory-compliant analytical results.

Whether you require isotope-labeled standards for pharmacokinetic studies, bioanalytical method validation, metabolite quantification, or LC-MS/MS assay development, ResolveMass Laboratories offers technical expertise and dependable scientific support to accelerate your research programs.

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