Methyl parathion-d6 (dimethyl-d6) | CAS 96740-32-8

OVERVIEW
Methyl parathion-d₆ (dimethyl-d₆) is a fully deuterated isotopologue of methyl parathion, a well-known organophosphate insecticide historically used for controlling a broad spectrum of agricultural pests. In this isotopically labeled form, all six hydrogen atoms of the two methyl groups in the parent compound are replaced by deuterium, giving it a molecular mass shift of +6 Da while maintaining identical physicochemical and chromatographic behavior to native methyl parathion.

This compound is primarily used as a stable isotope-labeled internal standard in analytical applications, particularly for quantifying methyl parathion residues in environmental, biological, and food samples via gas chromatography–mass spectrometry (GC-MS) or liquid chromatography–mass spectrometry (LC-MS). Methyl parathion-d₆ offers enhanced analytical precision, as its isotopic difference allows correction for matrix effects and sample preparation losses without altering the compound’s behavior during analysis.

CHEMICAL INFORMATION

• Name: Methyl parathion-d₆

• Molecular Formula: C₈D₆NO₅PS

• Molecular Weight: 269.24 g/mol

• CAS Number: 96740-32-8

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated organophosphate pesticide

• Stability: Stable under recommended storage conditions; decomposes upon prolonged exposure to heat, light, or moisture

APPLICATIONS  of  Methyl parathion-d6 (dimethyl-d6) | CAS 96740-32-8

• Analytical Chemistry (GC-MS / LC-MS Internal Standard):
  Methyl parathion-d₆ is extensively used as an isotopically labeled internal standard for the quantitative determination of methyl parathion residues in complex matrices. Its +6 Da isotopic shift allows clear distinction in mass spectra while ensuring identical chromatographic performance, enabling accurate quantification even at trace concentrations.

• Environmental Monitoring:
  Used in testing laboratories to detect and measure methyl parathion residues in environmental samples such as soil, surface water, and groundwater. It supports pollution studies and regulatory compliance monitoring for pesticide contamination.

• Food and Agricultural Testing:
  Applied in pesticide residue analysis of crops, grains, fruits, and vegetables to ensure compliance with international safety standards. The deuterated analog enhances analytical reliability for trace-level detection in complex food matrices.

• Toxicological and Metabolic Research:
  Serves as a stable isotope tracer in metabolic and toxicokinetic studies to evaluate the degradation, distribution, and bioaccumulation of organophosphate pesticides. Its isotopic labeling allows scientists to track compound transformations in biological systems.

• Quality Control and Method Validation:
  Employed in analytical laboratories as a calibration and method validation reference to assess instrument performance and method precision in organophosphate residue assays.

ADVANTAGES  of  Methyl parathion-d6 (dimethyl-d6) | CAS 96740-32-8

• Fully deuterated (+6 Da shift) for clear mass spectral separation

• Chemically identical to methyl parathion for consistent chromatographic performance

• High isotopic enrichment (≥98 atom % D) ensures analytical reliability

• Non-radioactive and stable, suitable for long-term laboratory use

• Enables high-accuracy quantification and reproducibility in trace-level detection

HANDLING

• Hazards: Highly toxic organophosphate compound; potent inhibitor of acetylcholinesterase. Exposure can cause severe poisoning even at low concentrations. Handle only in fume hoods with strict laboratory controls.

• Precautions: Use chemical-resistant gloves, protective eyewear, and lab coats. Avoid all forms of direct contact, inhalation, or ingestion. Ensure proper ventilation at all times.

• Storage: Keep sealed in amber containers at 2–8 °C under an inert atmosphere. Avoid exposure to heat, light, and oxidizing agents.

• Disposal: Dispose of contaminated materials and residues as hazardous waste according to institutional and environmental safety regulations for organophosphates.

Safety Note: Deuteration does not alter the biological or toxicological properties of methyl parathion; methyl parathion-d₆ remains highly toxic and should be handled using strict carcinogen and neurotoxin safety protocols.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.05 %

• Analytical Verification: Confirmed by ¹H NMR, ²H NMR, FT-IR, and high-resolution mass spectrometry (HRMS)

• COA Provided: Certificate of Analysis available with each batch confirming isotopic purity and chemical identity

SUMMARY
Methyl parathion-d₆ (dimethyl-d₆) (CAS 96740-32-8) is a high-purity, stable isotope-labeled analog of methyl parathion, used as an internal standard for analytical quantification of pesticide residues in food, environmental, and biological samples. Its deuterium enrichment provides precise isotopic differentiation, allowing highly accurate GC-MS and LC-MS analyses. Although invaluable for analytical and research purposes, methyl parathion-d₆ remains extremely toxic and must be handled with the utmost caution under controlled laboratory conditions.