Nicotinic-d4 Acid | CAS 66148-15-0

OVERVIEW
Nicotinic-d₄ acid is a stable isotope-labeled form of nicotinic acid (commonly known as niacin or vitamin B₃), in which four hydrogen atoms are replaced by deuterium. This isotopic substitution slightly increases its molecular mass (+4 Da) without altering its fundamental chemical structure, biological activity, or physicochemical behavior. Owing to this structural equivalence, nicotinic-d₄ acid serves as a powerful analytical standard and tracer molecule in biochemical, nutritional, and metabolic studies.

As a deuterated analog of nicotinic acid, this compound is particularly valuable in mass spectrometric quantification, pharmacokinetic profiling, and metabolic pathway tracing. It allows for precise distinction from the unlabeled form, enabling accurate quantification of niacin and its derivatives in biological matrices. Researchers often employ nicotinic-d₄ acid in the study of vitamin metabolism, coenzyme synthesis (NAD⁺/NADP⁺), and nutritional biochemistry.

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CHEMICAL INFORMATION

• Name: Nicotinic-d₄ acid

• Molecular Formula: C₆D₄NO₂

• Molecular Weight: 127.14 g/mol

• CAS Number: 66148-15-0

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated heteroaromatic carboxylic acid; stable isotope-labeled vitamin derivative

• Stability: Chemically stable under standard laboratory storage conditions; non-volatile and non-hygroscopic

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APPLICATIONS  of  Nicotinic-d4 Acid | CAS 66148-15-0

• Analytical Chemistry & Mass Spectrometry:
  Nicotinic-d₄ acid is extensively used as an internal standard in LC-MS and GC-MS analysis for the quantitative determination of nicotinic acid in biological fluids, food products, and pharmaceuticals. Its isotopic shift allows precise differentiation from endogenous niacin.

• Metabolic & Nutritional Research:
  Functions as a tracer molecule to investigate niacin metabolism, NAD⁺/NADP⁺ biosynthesis, and vitamin B₃ bioavailability in humans and model organisms.

• Pharmacokinetic and Biochemical Studies:
  Enables accurate tracking of absorption, distribution, and excretion of niacin-based therapeutics. Useful in identifying metabolic conversion rates and pathways under physiological and experimental conditions.

• Stable Isotope Labeling Experiments (SILE):
  Employed in isotope dilution studies for quantifying trace vitamin levels and assessing nutritional sufficiency in biological and food systems.

• Quality Control and Calibration:
  Serves as a calibration compound for analytical assays involving nicotinic acid and related metabolites, ensuring reproducible quantification and validation of analytical methods.

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ADVANTAGES  of  Nicotinic-d4 Acid | CAS 66148-15-0

• Excellent isotopic purity ensures analytical precision.

• Chemically identical to native nicotinic acid—ideal for comparative metabolic studies.

• Non-radioactive, safe, and environmentally stable isotope tracer.

• Enhances accuracy in LC-MS/MS quantification of vitamins and coenzymes.

• Valuable for nutritional, clinical, and pharmaceutical research involving vitamin B₃.

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HANDLING

• Hazards: Considered low-toxicity; may cause mild irritation upon contact or inhalation.

• Precautions: Handle using standard laboratory protective measures—wear gloves, goggles, and lab coat. Avoid inhalation of dust and contact with eyes.

• Storage: Store in tightly closed containers in a cool, dry location. Protect from excessive light and humidity.

• Disposal: Dispose of in accordance with local environmental and chemical safety regulations for non-hazardous organic compounds.

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QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Analytical Verification: Confirmed by ¹H NMR, ²H NMR, LC-MS, and IR spectroscopy

• Appearance: White crystalline or powdery solid

• COA Availability: Each batch supplied with Certificate of Analysis verifying isotopic composition and chemical purity

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SUMMARY
Nicotinic-d₄ acid (CAS 66148-15-0) is a high-purity, deuterium-labeled analog of nicotinic acid, widely employed as an isotopic internal standard and metabolic tracer in analytical and nutritional research. Its deuterium enrichment allows for precise detection and quantification of niacin and its metabolites, contributing to advancements in vitamin B₃ biochemistry, drug metabolism, and clinical nutrition studies.

 

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