Octanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-24-9

OVERVIEW  of  Octanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-24-9
Octanoyl-L-Carnitine-d₃ HCl (N-methyl-d₃) is a stable isotopically labeled analog of octanoyl-L-carnitine, a medium-chain acylcarnitine that plays a vital role in the transport of fatty acids into mitochondria for β-oxidation. In this labeled compound, the three hydrogen atoms on the N-methyl group of the L-carnitine moiety are substituted with deuterium atoms, yielding a mass shift of +3 Da. This deuteration preserves all chemical and biochemical characteristics of the native molecule, allowing it to serve as a precise internal standard for analytical quantitation in complex biological samples.

It is primarily utilized in targeted metabolomics, lipidomics, and clinical biochemistry to support accurate measurement of acylcarnitines through advanced LC-MS/MS methods, improving precision and reproducibility in metabolic profiling studies.

CHEMICAL INFORMATION

• Name: Octanoyl-L-Carnitine-d₃ hydrochloride

• Molecular Formula: C₁₅H₂₇D₃NO₄·HCl

• Molecular Weight: 326.88 g/mol

• CAS Number: 1334532-24-9

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated acylcarnitine; stable isotope-labeled metabolite

• Stability: Stable at room temperature under dry, inert conditions; hygroscopic in nature

APPLICATIONS   of  Octanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-24-9

• Targeted Metabolomics and Lipidomics:
  Extensively used as a stable isotope-labeled internal standard for quantification of native octanoyl-L-carnitine and related acylcarnitines by LC-MS/MS. It ensures analytical accuracy and compensates for matrix effects and ion suppression in complex biological samples.

• Clinical Diagnostics:
  Plays an important role in biochemical screening for fatty acid oxidation disorders (FAODs) and mitochondrial dysfunctions through plasma or urine acylcarnitine profiling.

• Biochemical and Nutritional Research:
  Enables detailed analysis of energy metabolism and lipid transport pathways, assisting researchers in studying mitochondrial β-oxidation mechanisms.

• Quality Control in Analytical Laboratories:
  Serves as a reference material or calibration standard to validate chromatographic performance, retention time reproducibility, and mass spectral accuracy.

• Pharmacokinetic and Metabolite Studies:
  Used to track metabolic flux and assess drug-induced changes in acylcarnitine concentrations during metabolic assays.

ADVANTAGES   of  Octanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-24-9

• High isotopic enrichment (≥ 98 atom % D) ensures clean mass spectral separation from endogenous species.

• Chemically identical behavior to the native form enables accurate quantitative comparisons.

• Non-radioactive, non-toxic, and environmentally safe for laboratory handling.

• Improves reliability and repeatability in analytical workflows.

HANDLING, STORAGE & SAFETY

• Handling Precautions: Use standard laboratory protective measures—gloves, lab coat, and eye protection. Avoid direct contact or inhalation.

• Storage: Maintain at –20 °C in a desiccated environment; keep away from light and moisture.

• Safety: Non-hazardous under normal laboratory use; handle according to good laboratory practices.

• Disposal: Dispose of waste according to institutional and regulatory guidelines for non-hazardous organic compounds.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Form: Crystalline or lyophilized powder

• Verification: Confirmed by LC-MS/MS and NMR spectroscopy

• Documentation: Supplied with Certificate of Analysis (CoA) specifying isotopic purity and analytical verification

SUMMARY
Octanoyl-L-Carnitine-d₃ HCl (N-methyl-d₃) | CAS 1334532-24-9 is a deuterium-labeled medium-chain acylcarnitine designed for quantitative LC-MS/MS metabolomics and clinical biochemical research. Its high isotopic purity and stability make it an ideal internal standard for measuring acylcarnitine concentrations in plasma, serum, and tissue samples. By mimicking the chromatographic and ionization behavior of the native compound, this reagent ensures high accuracy, reproducibility, and analytical precision in studies of fatty acid metabolism, mitochondrial function, and energy balance.