Propionyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 119793-66-7

OVERVIEW  of  Propionyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 119793-66-7
Propionyl-L-Carnitine-d₃ HCl (N-methyl-d₃) is a stable isotopically labeled analog of propionyl-L-carnitine, a short-chain acylcarnitine that plays a critical role in mitochondrial energy metabolism and fatty acid β-oxidation. In this deuterated variant, three hydrogen atoms on the N-methyl group of the L-carnitine moiety are replaced with deuterium, resulting in a +3 Da molecular mass shift. This isotopic substitution preserves the native compound’s physicochemical and biochemical properties, enabling its use as a precise internal standard in LC-MS/MS-based metabolomic and lipidomic analyses.

Propionyl-L-Carnitine-d₃ HCl is widely employed in clinical diagnostics, metabolic research, and mass spectrometry calibration, ensuring accurate quantification of acylcarnitine species and improving analytical reproducibility across biological samples.

CHEMICAL INFORMATION

• Name: Propionyl-L-Carnitine-d₃ hydrochloride

• Molecular Formula: C₁₀H₁₇D₃NO₄·HCl

• Molecular Weight: 256.74 g/mol

• CAS Number: 119793-66-7

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated acylcarnitine; stable isotope-labeled metabolite

• Stability: Stable under inert and dry storage conditions; hygroscopic in ambient humidity

APPLICATIONS  of  Propionyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 119793-66-7

• Targeted Metabolomics & Lipidomics:
  Utilized as a stable isotope-labeled internal standard for quantifying propionyl-L-carnitine and related acylcarnitines in plasma, serum, or tissue extracts using LC-MS/MS techniques.

• Clinical & Diagnostic Research:
  Applied in the profiling of acylcarnitines for the detection of inborn errors of metabolism (IEMs) and fatty acid oxidation disorders (FAODs).

• Energy Metabolism & Mitochondrial Function Studies:
  Supports research into short-chain fatty acid oxidation, mitochondrial activity, and cellular energy transport mechanisms.

• Mass Spectrometry Calibration & Quality Control:
  Serves as a reference compound for calibration of retention time, ionization response, and mass accuracy in targeted metabolomic assays.

• Pharmaceutical & Biochemical Research:
  Assists in drug metabolism studies and in the evaluation of therapeutic carnitine derivatives for mitochondrial and metabolic diseases.

ADVANTAGES  of  Propionyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 119793-66-7

• High isotopic enrichment (≥ 98 atom % D) ensures clear mass spectral separation from unlabeled analogs.

• Identical chromatographic and ionization properties to the native compound enable accurate quantification.

• Chemically stable, non-radioactive, and safe for laboratory use.

• Enhances accuracy, reproducibility, and data consistency in metabolomic workflows.

HANDLING, STORAGE & SAFETY

• Handling Precautions: Handle under standard laboratory safety protocols. Avoid inhalation, ingestion, or prolonged exposure to moisture.

• Storage: Maintain at –20 °C in a desiccated, airtight container.

• Safety: Non-hazardous and non-toxic under standard analytical use conditions.

• Disposal: Dispose according to local regulations for non-hazardous organic laboratory materials.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Form: Crystalline or amorphous solid

• Analytical Verification: Characterized and confirmed via LC-MS/MS and NMR spectroscopy

• Documentation: Supplied with Certificate of Analysis (CoA) confirming isotopic enrichment and analytical purity

SUMMARY
Propionyl-L-Carnitine-d₃ HCl (N-methyl-d₃) | CAS 119793-66-7 is a deuterium-labeled short-chain acylcarnitine used as a quantitative internal standard in targeted metabolomics, lipidomics, and clinical assays. It provides high isotopic purity, structural equivalence to the native compound, and excellent chromatographic consistency, ensuring precise quantification of acylcarnitines in complex biological matrices. This compound is an essential tool for studies involving energy metabolism, fatty acid oxidation, and mitochondrial bioenergetics, offering high analytical reliability and reproducibility.