Pyrazine-d4 | CAS 1758-62-9

OVERVIEW  of  Pyrazine-d4 | CAS 1758-62-9

Pyrazine-d₄ (CAS 1758-62-9) is a fully deuterated analog of pyrazine, a six-membered aromatic heterocycle containing two nitrogen atoms in para positions. The incorporation of four deuterium atoms replaces the hydrogen atoms on the carbon framework, making this compound ideal for use in isotope labeling, mass spectrometry (MS), and nuclear magnetic resonance (NMR) studies. Pyrazine-d₄ maintains the same chemical reactivity and structural characteristics as non-deuterated pyrazine, ensuring accurate comparative and quantitative analyses.

CHEMICAL INFORMATION

• Name: Pyrazine-d₄

• Molecular Formula: C₄D₄N₂

• Molecular Weight: 84.11 g/mol

• CAS Number: 1758-62-9

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated aromatic heterocycle

• Solubility: Soluble in water and most organic solvents (ethanol, methanol, ether)

APPLICATIONS  of  Pyrazine-d4 | CAS 1758-62-9

• Nuclear Magnetic Resonance (NMR):
  Pyrazine-d₄ is widely used as a reference standard or solvent additive in NMR spectroscopy, minimizing proton interference and enhancing spectral clarity.

• Mass Spectrometry (MS):
  Serves as an internal standard or isotopic reference for quantitative MS analyses of pyrazine derivatives and related compounds.

• Isotope Labeling Studies:
  Useful as a stable isotope tracer in studies investigating reaction mechanisms, biotransformation, and environmental fate of nitrogen-containing heterocycles.

• Synthetic Chemistry:
  Acts as a deuterated building block for synthesizing labeled intermediates, pharmaceuticals, and advanced materials for mechanistic and metabolic research.

• Flavor and Fragrance Research:
  Pyrazine derivatives are key contributors to roasted, nutty, and earthy flavors; Pyrazine-d₄ aids in pathway tracing and analytical quantification of such compounds in food chemistry.

ADVANTAGES  of  Pyrazine-d4 | CAS 1758-62-9

• High isotopic purity and chemical stability.

• Structurally identical to native pyrazine, enabling accurate comparative studies.

• Non-radioactive and environmentally safe isotope label.

• Provides enhanced analytical precision in MS and NMR applications.

• Excellent tool for mechanistic and quantitative investigations.

HANDLING AND SAFETY

• Handling: Avoid breathing vapors and direct contact with skin or eyes. Use only in well-ventilated areas.

• Storage: Keep container tightly closed in a cool, dry, and dark environment.

• Safety: Mild irritant; wear standard laboratory protective equipment (gloves, goggles, coat).

• Disposal: Dispose of in accordance with institutional and local chemical waste guidelines.

SUMMARY
Pyrazine-d₄ (CAS 1758-62-9) is a high-purity deuterium-labeled aromatic heterocycle extensively employed in analytical chemistry, isotopic labeling, and structural studies. Its stability, deuterium enrichment, and chemical equivalence to pyrazine make it indispensable for quantitative NMR, MS calibration, and reaction mechanism research across chemical, pharmaceutical, and flavor science disciplines.

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