Relpivirine-d6 | CAS No. 1312424-26-2

Relpivirine-d6 is a high-purity stable isotope-labeled analogue of Relpivirine, a non-nucleoside reverse transcriptase inhibitor (NNRTI) widely used in antiretroviral therapy for HIV-1 infection. In Relpivirine-d6, six hydrogen atoms are replaced with deuterium (^2H), resulting in a mass shift of +6 Da while preserving nearly identical chemical and chromatographic behavior to the unlabeled compound. This makes Relpivirine-d6 an ideal internal standard for quantitative analysis using LC-MS/MS, UHPLC-MS/MS, and LC-HRMS.

At ResolveMass Laboratories Inc., we supply premium-quality isotope-labeled reference standards that meet the analytical demands of pharmaceutical companies, contract research organizations (CROs), academic institutions, and clinical research laboratories. Relpivirine-d6 is intended for research use only (RUO) and is commonly used in bioanalytical method development, pharmacokinetic studies, bioequivalence testing, therapeutic drug monitoring, and drug metabolism research.

Product Information

Product Description

Relpivirine-d6 is specifically designed to serve as an internal standard for the accurate quantification of Relpivirine in complex biological matrices. Since the labeled compound exhibits virtually identical extraction efficiency, chromatographic retention, and ionization characteristics as the native analyte, it effectively compensates for analytical variability introduced during sample preparation and instrumental analysis.

The incorporation of six deuterium atoms allows mass spectrometers to distinguish Relpivirine-d6 from the unlabeled drug without affecting its physicochemical properties. Consequently, isotope dilution mass spectrometry using Relpivirine-d6 significantly improves assay accuracy, precision, and reproducibility.

Modern bioanalytical laboratories routinely employ Relpivirine-d6 for regulated studies conducted according to FDA, EMA, and ICH M10 Bioanalytical Method Validation guidelines. Its excellent analytical performance makes it an indispensable reference standard for pharmaceutical development and clinical research.

Key Features

• High-purity stable isotope-labeled reference standard
• Six deuterium atoms (D6) incorporated into the parent molecule
• Excellent chromatographic co-elution with Relpivirine
• Distinct mass shift (+6 Da) for accurate MS detection
• Suitable for LC-MS/MS, UHPLC-MS/MS, and LC-HRMS methods
• Minimizes matrix effects and ion suppression
• Corrects extraction variability and instrument fluctuations
• Supports highly accurate quantitative bioanalysis
• Ideal for regulated pharmaceutical research
• Manufactured for research applications only

Applications

LC-MS/MS Bioanalysis

Relpivirine-d6 is widely used as an internal standard during quantitative LC-MS/MS analysis of Relpivirine in plasma, serum, whole blood, and other biological matrices. Its close structural similarity to the analyte ensures consistent recovery throughout extraction and analysis, enabling highly reproducible results.

Bioanalytical Method Development

Analytical scientists use Relpivirine-d6 while developing sensitive and selective analytical methods for pharmaceutical research. The isotope-labeled standard assists in optimizing chromatographic conditions, extraction procedures, and mass spectrometric parameters for reliable quantification.

Bioanalytical Method Validation

Relpivirine-d6 supports comprehensive method validation studies by helping evaluate critical analytical parameters, including:

• Accuracy
• Precision
• Selectivity
• Recovery
• Matrix effect
• Linearity
• Stability
• Carryover
• Dilution integrity
• Lower Limit of Quantification (LLOQ)

Its use enhances method robustness and compliance with global regulatory expectations.

Pharmacokinetic (PK) Studies

Relpivirine-d6 plays a significant role in pharmacokinetic studies by enabling precise measurement of drug concentrations across multiple sampling time points. Reliable quantification is essential for calculating pharmacokinetic parameters such as:

• Maximum plasma concentration (Cmax)
• Time to reach maximum concentration (Tmax)
• Area Under the Curve (AUC)
• Elimination half-life (t½)
• Clearance (CL)
• Volume of distribution (Vd)

Bioequivalence Studies

Generic drug manufacturers utilize Relpivirine-d6 in bioequivalence studies to compare the pharmacokinetic performance of generic and reference formulations. The isotope-labeled internal standard minimizes analytical variability and improves confidence in study results.

Drug Metabolism and Clinical Research

Relpivirine-d6 is also employed in drug metabolism investigations, therapeutic drug monitoring research, clinical pharmacology studies, biomarker research, and translational medicine projects requiring highly accurate quantitative analysis.

Advantages of Using Relpivirine-d6

Stable isotope-labeled internal standards provide superior analytical performance compared to structural analogues or external standards. Relpivirine-d6 offers several important advantages:

• Compensates for extraction losses during sample preparation
• Corrects matrix-induced ion suppression and ion enhancement
• Reduces analytical variability
• Improves assay accuracy and precision
• Enhances reproducibility across analytical batches
• Supports reliable quantification at low concentration levels
• Improves regulatory compliance in bioanalytical studies
• Provides excellent long-term analytical consistency

These benefits make Relpivirine-d6 the preferred internal standard for quantitative mass spectrometric analysis.

Compatible Analytical Techniques

Relpivirine-d6 is suitable for use with various advanced analytical platforms, including:

• LC-MS/MS
• UHPLC-MS/MS
• LC-HRMS
• Triple Quadrupole Mass Spectrometry
• Orbitrap Mass Spectrometry
• Quadrupole Time-of-Flight (QTOF) Mass Spectrometry
• Stable Isotope Dilution Assays

Research Areas

Relpivirine-d6 supports a wide range of pharmaceutical and analytical research applications, including:

• Pharmaceutical Research
• Bioanalytical Testing
• Clinical Research
• Pharmacokinetic Studies
• Bioequivalence Studies
• Drug Metabolism Research
• Therapeutic Drug Monitoring
• Generic Drug Development
• Analytical Method Development
• Method Validation
• Regulatory Submission Studies
• Contract Research Organizations (CROs)
• Academic Research
• Quality Control Laboratories

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. specializes in supplying high-quality stable isotope-labeled reference standards for pharmaceutical, biotechnology, and analytical laboratories worldwide. Our products are manufactured to support demanding analytical workflows and provide consistent, reproducible performance in regulated research environments.

By combining expertise in mass spectrometry, isotope chemistry, and pharmaceutical analysis, we help researchers develop robust analytical methods that meet the expectations of FDA, EMA, Health Canada, and ICH guidelines.

Whether you are conducting pharmacokinetic studies, bioequivalence testing, bioanalytical method validation, or LC-MS/MS assay development, ResolveMass Laboratories Inc. provides reliable isotope-labeled standards to support your analytical success.

Why Researchers Trust ResolveMass

• Premium-quality stable isotope-labeled reference standards
• High isotopic enrichment and chemical purity
• Batch-to-batch consistency
• Comprehensive analytical characterization
• Reliable technical support
• Fast global delivery
• Trusted partner for pharmaceutical and biotechnology research

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