Sodium butyrate-d7 | CAS 270564-49-3

OVERVIEW  of  Sodium butyrate-d7 | CAS 270564-49-3

Sodium butyrate-d₇ is a fully deuterated analog of sodium butyrate, a short-chain fatty acid (SCFA) salt widely studied for its biological and metabolic significance. With seven deuterium atoms replacing hydrogen in the butyrate structure, this stable isotope-labeled compound offers a distinctive mass shift while retaining identical physicochemical behavior to its unlabeled form.

Sodium butyrate itself is a key metabolic intermediate produced through microbial fermentation of dietary fibers in the gut and plays an essential role in regulating energy metabolism, gene expression, and cellular differentiation. The deuterated version, Sodium butyrate-d₇, is primarily used as an internal standard, metabolic tracer, and quantitative reference in analytical chemistry, biomedical research, and metabolomics. Its exceptional isotopic purity and stability make it indispensable for high-precision analysis using GC-MS, LC-MS, and NMR spectroscopy.

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CHEMICAL INFORMATION

• Name: Sodium butyrate-d₇

• CAS Number: 270564-49-3

• Molecular Formula: C₄D₇NaO₂

• Molecular Weight: 117.12 g/mol

• Isotopic Enrichment: ≥98 atom % D

• Chemical Class: Deuterated short-chain fatty acid salt

• Solubility: Highly soluble in water and ethanol

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APPLICATIONS  of  Sodium butyrate-d7 | CAS 270564-49-3

1. Metabolic and Isotopic Tracing:
Sodium butyrate-d₇ is widely used in metabolic studies to trace the biosynthesis, oxidation, and utilization of short-chain fatty acids. It enables precise measurement of butyrate turnover, absorption, and metabolic fate in cell cultures, animal models, and human studies, offering key insights into gut microbiome function and host metabolism.

2. Internal Standard for Quantitative Analysis:
The compound serves as an ideal internal standard for the quantification of endogenous and exogenous butyrate in biological fluids such as plasma, serum, urine, and fecal extracts. The deuterium label provides a clear isotopic shift that enhances analytical reliability in GC-MS and LC-MS/MS methods.

3. Gut Microbiome and SCFA Profiling:
As a deuterated analog of a major microbial metabolite, Sodium butyrate-d₇ is extensively applied in gut microbiome research. It supports studies on short-chain fatty acid biosynthesis, microbial fermentation kinetics, and host–microbiota interactions, allowing researchers to quantify butyrate fluxes in complex biological systems.

4. Epigenetic and Cellular Research:
Butyrate is a known histone deacetylase (HDAC) inhibitor, influencing gene expression, apoptosis, and differentiation. The deuterated version enables detailed isotopic labeling studies that explore the molecular mechanisms of butyrate’s biological actions at the cellular level.

5. Environmental and Food Analysis:
In addition to biomedical applications, Sodium butyrate-d₇ functions as a calibration and reference standard in environmental and food chemistry for measuring volatile fatty acids in fermentation broths, beverages, and soil extracts.

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ADVANTAGES  of  Sodium butyrate-d7 | CAS 270564-49-3

• High Deuterium Content (≥98 atom % D): Provides distinct isotopic separation for precision quantitation.

• Chemically Equivalent to Native Sodium Butyrate: Ensures identical behavior in extraction and chromatographic separation.

• Excellent Stability and Solubility: Suitable for aqueous systems, biological media, and standard preparation.

• Non-Radioactive and Environmentally Safe: Safe for regular laboratory use.

• Ideal for Mass Spectrometry: Compatible with GC-MS, LC-MS, and isotope-ratio MS analyses.

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SUMMARY
Sodium butyrate-d₇ (CAS 270564-49-3) is a fully deuterated, stable isotope-labeled compound designed for advanced analytical, biochemical, and metabolic studies. It serves as an essential tool in quantifying butyrate and other short-chain fatty acids across a range of biological and environmental systems. By replacing hydrogen with deuterium, the compound exhibits a mass difference that enables high-resolution detection without altering chemical behavior, making it ideal for internal standardization and isotopic tracing.

In metabolic research, Sodium butyrate-d₇ enables the study of microbial fermentation processes, gut–liver axis regulation, and energy metabolism. In molecular biology, it supports research on epigenetic modulation through HDAC inhibition, providing a stable isotopic marker for elucidating biochemical pathways.

At ResolveMass Laboratories Inc., Sodium butyrate-d₇ is produced under rigorous quality standards to ensure high isotopic enrichment, purity, and batch-to-batch consistency. Its robustness, reproducibility, and analytical precision make it a preferred standard for scientists investigating the role of short-chain fatty acids in health, disease, and environmental systems.

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