Telaprevir-d4 | CAS 402957-28-2
Telaprevir-d4 is a stable isotope-labeled analog of Telaprevir, a first-generation direct-acting antiviral (DAA) that inhibits the Hepatitis C Virus (HCV) NS3/4A serine protease. The deuterium-labeled version contains four deuterium (^2H) atoms strategically incorporated into the molecule, making it an ideal internal standard (IS) for quantitative LC-MS/MS analysis. The isotopic substitution preserves the compound’s physicochemical and chromatographic characteristics while providing a distinct mass shift for accurate mass spectrometric detection.
Telaprevir-d4 is extensively used in pharmaceutical analysis, bioanalytical method development, pharmacokinetic (PK) studies, therapeutic drug monitoring (TDM), metabolism studies, and regulatory bioequivalence testing. Stable isotope-labeled standards such as Telaprevir-d4 help compensate for sample preparation losses, ion suppression, matrix effects, and instrumental variability, ensuring highly reproducible quantitative data across complex biological matrices.
At ResolveMass Laboratories Inc., we provide high-quality isotope-labeled reference standards to support pharmaceutical research, CRO services, bioanalytical assay validation, and regulatory submissions worldwide.
Product Overview
| Property | Details |
|---|---|
| Product Name | Telaprevir-d4 |
| CAS Number | 402957-28-2 (parent compound CAS) |
| Molecular Formula | C36H49D4N7O6 |
| Molecular Weight | 683.87 g/mol |
| Labeling | Four Deuterium Atoms (d4) |
| Parent Compound | Telaprevir |
| Drug Class | HCV NS3/4A Protease Inhibitor |
| Application | LC-MS/MS Internal Standard, Bioanalysis, Pharmacokinetics, Pharmaceutical Research |
| Research Use | Research Use Only (RUO) |
Specifications may vary depending on manufacturing batch and analytical characterization.
About Telaprevir
Telaprevir (VX-950) was among the first direct-acting antivirals developed to target chronic Hepatitis C virus genotype 1 infection. It acts by selectively inhibiting the viral NS3/4A protease, an enzyme essential for viral polyprotein processing and viral replication. Although newer antiviral regimens have largely replaced Telaprevir in clinical practice, it remains an important reference molecule in pharmaceutical research, analytical method development, impurity profiling, and bioanalytical investigations.
Because Telaprevir continues to be evaluated in pharmacological, metabolic, and analytical studies, reliable isotope-labeled standards remain essential for quantitative LC-MS/MS workflows.
Why Use Telaprevir-d4 as an Internal Standard?
Stable isotope-labeled compounds are considered the gold standard for quantitative mass spectrometry because they closely mimic the behavior of the native analyte throughout sample preparation and instrumental analysis.
Advantages of using Telaprevir-d4 include:
- Excellent correction for extraction variability
- Compensation for matrix effects during ionization
- Improved quantitative accuracy
- Enhanced assay precision
- Better reproducibility across analytical runs
- Reliable recovery correction
- Robust method validation according to regulatory expectations
- Minimal chromatographic shift relative to the native analyte
These characteristics make Telaprevir-d4 particularly valuable in regulated bioanalytical laboratories performing GLP-compliant and GCP-compliant studies.
Applications of Telaprevir-d4
LC-MS/MS Quantitative Bioanalysis
Telaprevir-d4 is widely used as an internal standard during quantitative LC-MS/MS determination of Telaprevir concentrations in biological matrices including:
- Human plasma
- Serum
- Whole blood
- Urine
- Tissue homogenates
- Cell culture media
The isotopic analog provides accurate normalization throughout sample preparation and instrumental analysis.
Pharmacokinetic Studies
Researchers employ Telaprevir-d4 during PK investigations to accurately determine:
- Drug absorption
- Plasma concentration profiles
- Distribution
- Clearance
- Elimination half-life
- Bioavailability
- Dose proportionality
High analytical precision is particularly important when evaluating generic formulations or modified-release drug products.
Bioequivalence Studies
Generic drug manufacturers rely on isotope-labeled internal standards to support:
- Bioequivalence (BE) studies
- Comparative pharmacokinetics
- Regulatory submissions
- Method validation
- Sample reanalysis
- Cross-validation between laboratories
Accurate quantification is critical for demonstrating equivalence between reference and test formulations.
Drug Metabolism Research
Telaprevir undergoes metabolic transformation within the body, producing multiple metabolites. Telaprevir-d4 assists researchers in distinguishing endogenous compounds from the parent drug while improving quantitative confidence during metabolite profiling studies.
Applications include:
- Metabolite identification
- Metabolic stability studies
- Enzyme phenotyping
- Drug-drug interaction investigations
- Hepatic microsomal assays
Pharmaceutical Quality Control
Analytical laboratories use Telaprevir-d4 during:
- Method development
- Analytical method validation
- Accuracy studies
- Precision assessment
- Recovery experiments
- Stability studies
- Forced degradation investigations
- Laboratory quality assurance programs
Role in LC-MS/MS Method Development
During LC-MS/MS assay development, isotope-labeled internal standards significantly improve analytical performance by correcting variability introduced during:
- Protein precipitation
- Solid-phase extraction (SPE)
- Liquid-liquid extraction (LLE)
- Sample evaporation
- Reconstitution
- Chromatographic injection
- Electrospray ionization (ESI)
Since Telaprevir-d4 exhibits nearly identical chromatographic behavior to native Telaprevir, both compounds co-elute while remaining distinguishable based on their mass difference. This ensures highly reliable quantitative analysis even in challenging biological matrices.
Advantages of Stable Isotope-Labeled Standards
Compared with structural analogs, Telaprevir-d4 offers several analytical advantages:
- Identical extraction characteristics
- Comparable chromatographic retention
- Similar ionization efficiency
- Excellent analytical reproducibility
- Improved inter-day precision
- Reduced analytical bias
- Superior quantitative reliability
- Compliance with international bioanalytical method validation practices
These features make isotope-labeled standards the preferred choice for regulated pharmaceutical laboratories.
Research Areas Supported
Telaprevir-d4 supports numerous pharmaceutical and biomedical research applications, including:
- Bioanalytical assay development
- Clinical pharmacology
- Therapeutic drug monitoring
- Drug metabolism studies
- Pharmacokinetic evaluations
- Toxicokinetic investigations
- Generic drug development
- Regulatory bioanalysis
- CRO analytical services
- Biomarker research
- Pharmaceutical quality control
- Academic drug research
Why Choose ResolveMass Laboratories Inc.?
ResolveMass Laboratories Inc. is a trusted analytical partner providing high-quality isotope-labeled reference standards and advanced mass spectrometry solutions for pharmaceutical and biotechnology industries. Our experienced scientific team supports customers throughout the analytical workflow, from method development to regulatory-compliant bioanalysis.
Our expertise includes:
- Stable isotope-labeled reference standards
- LC-MS/MS method development and validation
- Pharmacokinetic and bioanalytical studies
- Impurity characterization
- Metabolite identification
- Pharmaceutical reference standards
- High-resolution mass spectrometry (HRMS)
- Custom analytical solutions
- Regulatory-compliant laboratory services
- CRO support for drug development
Our commitment to scientific excellence, quality assurance, and customer-focused service makes ResolveMass Laboratories Inc. a reliable partner for pharmaceutical research and analytical testing.
Whether you are developing bioanalytical assays, validating LC-MS/MS methods, conducting pharmacokinetic studies, or supporting generic drug development, Telaprevir-d4 (CAS 402957-28-2) provides the analytical accuracy and reliability required for high-quality quantitative mass spectrometry applications.
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