Tetradecanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-25-0

OVERVIEW  of  Tetradecanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-25-0

Tetradecanoyl-L-Carnitine-d₃ HCl (N-methyl-d₃) is a deuterium-labeled analog of tetradecanoyl-L-carnitine, a long-chain acylcarnitine ester involved in fatty acid transport and mitochondrial β-oxidation. In this compound, the three hydrogen atoms of the N-methyl group are replaced with deuterium (d₃), resulting in a +3 Da molecular weight shift compared to the unlabeled form. This isotopic substitution preserves the biochemical and chromatographic behavior of the parent molecule while allowing for precise differentiation in analytical studies.

Tetradecanoyl-L-Carnitine-d₃ HCl is widely used in metabolomics, clinical biochemistry, and mass spectrometric quantification of acylcarnitines. It serves as an internal standard for targeted lipidomics and bioanalytical assays involving mitochondrial metabolism and fatty acid oxidation disorders.

CHEMICAL INFORMATION

• Name: Tetradecanoyl-L-Carnitine-d₃ hydrochloride;

• Molecular Formula: C₂₁H₃₉D₃NO₄·HCl

• Molecular Weight: 426.03 g/mol

• CAS Number: 1334532-25-0

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated acylcarnitine; lipid metabolism intermediate analog

• Stability: Stable under recommended storage; hygroscopic in nature

APPLICATIONS  of  Tetradecanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-25-0

• Metabolomics and Lipidomics Studies:
  Tetradecanoyl-L-Carnitine-d₃ HCl is commonly employed as a stable isotope-labeled internal standard in LC-MS/MS-based metabolomic assays for profiling long-chain acylcarnitines in plasma, serum, urine, and tissue extracts.

• Quantitative Bioanalysis:
  Utilized in targeted quantification of tetradecanoyl-L-carnitine and related acylcarnitines in biological fluids to assess mitochondrial function, β-oxidation activity, and fatty acid metabolism disorders.

• Clinical Diagnostics and Research:
  Plays a crucial role in newborn screening and metabolic disease research, aiding in the identification of disorders such as fatty acid oxidation defects and carnitine transport deficiencies.

• Mass Spectrometry Calibration and Validation:
  Serves as an isotopically matched internal standard for high-precision MS quantification, ensuring accurate calibration and reducing matrix effects in complex biological samples.

• Pharmacokinetic and Biochemical Studies:
  Used in research exploring lipid metabolism, energy utilization, and the transport of fatty acids across mitochondrial membranes under labeled-tracer conditions.

ADVANTAGES  of  Tetradecanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-25-0

• High isotopic enrichment (≥ 98 atom % D) ensuring distinct MS signals and accurate quantitation.

• Chemically and chromatographically equivalent to native tetradecanoyl-L-carnitine.

• Ideal for LC-MS/MS analysis of acylcarnitine profiles in clinical and biochemical samples.

• Non-radioactive and stable isotope label suitable for safe handling and storage.

• Enables reproducible, precise, and traceable metabolic quantification in research and diagnostics.

HANDLING, STORAGE & SAFETY

• Hazards: Generally considered non-hazardous in small laboratory quantities; handle according to good laboratory practices.

• Precautions: Use gloves, goggles, and lab coat; avoid moisture and direct sunlight exposure.

• Storage: Store at –20 °C in a desiccated environment, away from strong oxidizers or reactive chemicals.

• Disposal: Dispose of in accordance with local chemical waste disposal regulations.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Form: Crystalline powder or lyophilized solid

• Verification Methods: LC-MS/MS, ¹H NMR (absence of proton peaks), and ²H NMR (confirmation of deuterium substitution)

• Documentation: Certificate of Analysis (CoA) provided with detailed purity and isotopic data

SUMMARY
Tetradecanoyl-L-Carnitine-d₃ HCl (N-methyl-d₃) | CAS 1334532-25-0 is a deuterium-labeled acylcarnitine designed for quantitative metabolomic and lipidomic studies. Its isotopic labeling allows for precise internal standardization in LC-MS/MS assays, improving accuracy in the measurement of long-chain acylcarnitines in biological matrices. With high isotopic purity, excellent solubility, and chemical stability, this compound is indispensable for biochemical research, clinical diagnostics, and energy metabolism investigations involving carnitine-dependent fatty acid transport.

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