uracil-5,6-d2 | CAS 24897-52-7

OVERVIEW

uracil-5,6-d2 | CAS 24897-52-7 is a high-purity, deuterium-labeled nucleobase specifically engineered for use in advanced analytical, biochemical, and pharmaceutical research. As an isotopically enriched form of uracil—one of the four key nucleobases found in RNA—this compound features two deuterium atoms substituted at the 5 and 6 positions of the pyrimidine ring. These stable isotopic labels make Uracil-5,6-d₂ a valuable internal standard, tracer molecule, and mechanistic probe across diverse scientific applications.

At ResolveMass Laboratories Inc., we provide research-grade Uracil-5,6-d₂ with exceptional isotopic enrichment and chemical purity. This ensures accurate quantitation, clean mass-spectrometric behavior, and reproducibility in high-sensitivity workflows such as LC–MS/MS, HRMS, and NMR.

CHEMICAL INFORMATION

• Product Name: Uracil-5,6-d₂

• CAS Number: 24897-52-7

• Molecular Formula: C₄H₂D₂N₂O₂

• Molecular Weight: 114.04 g/mol

• Isotopic Labeling: Deuterium at the 5 and 6 carbon positions

• Synonyms:

  • 5,6-Dideuterouracil

  • Uracil-d₂

  • [5,6-²H₂]Uracil

• Category: Stable isotopically labeled nucleobase

Uracil-5,6-d₂ maintains the same chemical reactivity, tautomeric behavior, and hydrogen-bonding capability as natural uracil, with the deuterium labels incorporated non-perturbatively into the ring structure. This conserves biological and chemical relevance while enabling precise isotopic discrimination in analytical systems.

KEY FEATURES

1. High Isotopic Enrichment
   Each molecule contains two deuterium atoms located at positions 5 and 6, enabling excellent mass separation from the non-labeled uracil signal during HRMS analysis.

2. Enhanced Analytical Accuracy
   Ideal as an internal standard for quantitative LC–MS and GC–MS assays involving pyrimidines, nucleosides, or metabolic pathways associated with RNA turnover.

3. Stable and Non-Radioactive
   Deuterium labeling ensures long-term stability, safe handling, and no regulatory burden associated with radioactive isotopes.

4. Compatible With Biological and Chemical Systems
   Deuterium substitution minimally perturbs biochemical behavior, making this molecule suitable for mechanistic studies, isotope tracing, and metabolic flux research.

APPLICATIONS  of  uracil-5,6-d2 | CAS 24897-52-7

1. Mass Spectrometry Internal Standard
Uracil-5,6-d₂ is widely used as an internal standard for the quantification of endogenous uracil in biological samples such as plasma, urine, cell lysates, and tissue extracts. Its two-mass-unit shift relative to native uracil allows precise discrimination in LC–MS/MS, making it optimal for pharmacokinetic and biomarker studies.

2. Metabolic Pathway Tracing
Deuterium labeling enables researchers to track the incorporation, conversion, and degradation of pyrimidine bases in metabolic pathways. Uracil-5,6-d₂ is particularly useful in studies of:

• RNA biosynthesis and turnover

• Pyrimidine salvage pathways

• Nucleotide metabolism disorders

• Microbial metabolism and isotopic probing in microbiome research

Its stable isotopic signature helps determine reaction rates, map metabolic flux, and identify unknown biochemical intermediates.

3. Mechanistic and Enzymatic Studies
Enzymes involved in pyrimidine metabolism—such as dihydropyrimidine dehydrogenase, uridine phosphorylase, or orotate pathway enzymes—can be studied using Uracil-5,6-d₂ as a probe substrate. Deuterium kinetic isotope effects provide valuable mechanistic insights and help differentiate between competing reaction pathways.

4. NMR and Spectroscopic Studies
The presence of deuterium modifies NMR signals in predictable ways, enabling clearer spectral interpretation and simplification of proton coupling patterns. This facilitates structural studies, tautomer analysis, and conformational investigations.

5. Stable Isotope-Labeled Research Reagents
As synthetic biology, RNA therapeutics, and nucleotide-based diagnostics expand, isotopically enriched reagents like Uracil-5,6-d₂ support quality control, method validation, and advanced analytical workflows.

ADVANTAGES FOR RESEARCHERS

• Precision in Quantitative Bioanalysis: Deuterium labeling provides consistent signal intensity and excellent ionization behavior, improving assay reproducibility.

• Reduced Matrix Interference: The +2 amu shift ensures clean mass spectral separation even in complex biological matrices.

• Versatility Across Platforms: Suitable for LC–MS/MS, HRMS, GC–MS (after derivatization), NMR, and stable isotope-resolved metabolomics (SIRM).

• Trusted Quality from ResolveMass: Our isotopically labeled compounds undergo rigorous purity, isotopic enrichment, and identity testing to support high-impact research and regulated workflows.

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