Valproic Acid-d4 | CAS 87745-17-3

Valproic Acid-d4 (CAS No. 87745-17-3) is a stable isotope-labeled analogue of valproic acid in which four hydrogen atoms are replaced with deuterium. This isotopically labeled compound possesses nearly identical physicochemical properties to the native analyte while exhibiting a distinguishable mass shift during mass spectrometric analysis. As a result, Valproic Acid-d4 serves as an ideal internal standard for the accurate quantification of valproic acid in pharmaceutical formulations, biological matrices, bioanalytical studies, pharmacokinetic investigations, therapeutic drug monitoring (TDM), and forensic toxicology.

Stable isotope-labeled reference standards are essential for modern analytical laboratories because they compensate for sample preparation losses, matrix effects, ion suppression, extraction variability, and instrument fluctuations. By co-eluting with the target analyte while remaining distinguishable by mass spectrometry, Valproic Acid-d4 significantly improves analytical precision, accuracy, and reproducibility across validated LC-MS/MS and GC-MS methods.

Product Information

• Product Name: Valproic Acid-d4
• CAS Number: 87745-17-3
• Molecular Formula: C₈H₁₂D₄O₂
• Molecular Weight: 148.24 g/mol
• Synonyms: Valproate-d4, VPA-d4, 2-Propylvaleric Acid-d4
• Product Type: Stable Isotope-Labeled Reference Standard
• Application: Internal standard for LC-MS/MS and GC-MS quantitative analysis

Applications of Valproic Acid-d4 :

Valproic Acid-d4 is widely utilized in pharmaceutical research, analytical chemistry, clinical laboratories, and bioanalytical CRO environments. Its primary role is to ensure reliable quantification of valproic acid across a variety of analytical workflows.

Common applications include:

• LC-MS/MS bioanalytical method development and validation
• Quantitative therapeutic drug monitoring (TDM)
• Pharmacokinetic (PK) and toxicokinetic (TK) studies
• Bioequivalence (BE) and bioavailability (BA) studies
• Clinical and preclinical plasma sample analysis
• Forensic toxicology testing
• Pharmaceutical quality control
• Stability studies
• Drug metabolism research
• Method transfer and cross-laboratory validation

Why Use a Stable Isotope-Labeled Internal Standard?

Accurate quantitative mass spectrometry depends on controlling analytical variability. Since Valproic Acid-d4 closely mimics the chromatographic and extraction behavior of unlabeled valproic acid, it effectively corrects for:

• Matrix-induced ion suppression or enhancement
• Sample extraction losses
• Variations in injection volume
• Instrument response fluctuations
• Sample preparation inconsistencies
• Recovery differences between analytical batches

Because the labeled compound co-elutes with the analyte while maintaining a unique mass signature, it enables highly reliable isotope dilution mass spectrometry, which is widely regarded as the gold standard for quantitative bioanalysis.

Pharmaceutical and Bioanalytical Importance

Valproic acid is a well-established anticonvulsant and mood-stabilizing drug used in the treatment of epilepsy, bipolar disorder, and migraine prophylaxis. Accurate measurement of circulating drug concentrations is critical due to its relatively narrow therapeutic range and significant interpatient pharmacokinetic variability.

Valproic Acid-d4 supports highly sensitive quantitative assays that are routinely employed in:

• Clinical pharmacology studies
• Therapeutic drug monitoring laboratories
• Drug-drug interaction studies
• Pharmacokinetic profiling
• Generic drug development
• Biosample analysis during clinical trials

Its use improves confidence in regulatory submissions by providing accurate quantitative performance throughout validated analytical methods.

Advantages of Valproic Acid-d4

Researchers and analytical laboratories prefer Valproic Acid-d4 because it offers several important advantages:

• Excellent chromatographic matching with unlabeled valproic acid
• High analytical accuracy
• Improved assay precision
• Compensation for extraction variability
• Reduced matrix interference
• Enhanced reproducibility between analytical runs
• Compatibility with validated LC-MS/MS and GC-MS methods
• Ideal for isotope dilution quantitative analysis

These characteristics make the compound a valuable reference material for regulated pharmaceutical laboratories operating under GLP, GMP, and bioanalytical validation guidelines.

Analytical Techniques

Valproic Acid-d4 is compatible with numerous analytical platforms, including:

• Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
• Gas Chromatography-Mass Spectrometry (GC-MS)
• High-Resolution Mass Spectrometry (HRMS)
• Quantitative LC-MS workflows
• Isotope dilution mass spectrometry
• Clinical bioanalytical assays
• Pharmaceutical impurity investigations

Its excellent analytical performance makes it suitable for routine quantitative workflows in pharmaceutical R&D and contract research laboratories.

Research Use Only

Valproic Acid-d4 is intended exclusively for research and laboratory applications. It is not intended for therapeutic, diagnostic, or veterinary use. The material is designed to support pharmaceutical analysis, analytical method development, bioanalytical validation, and scientific research.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. supplies high-quality isotope-labeled reference standards that support pharmaceutical research, bioanalytical testing, and analytical method development. Our expertise in mass spectrometry, pharmaceutical analysis, and regulatory science enables us to provide reliable reference materials suitable for demanding analytical applications.

Whether your laboratory requires standards for LC-MS/MS quantification, pharmacokinetic studies, therapeutic drug monitoring, or pharmaceutical quality control, ResolveMass is committed to delivering dependable analytical solutions supported by technical expertise and rigorous quality standards.

For product inquiries, bulk quantities, custom analytical requirements, or technical support, contact ResolveMass Laboratories Inc. to discuss your research needs and analytical workflow requirements.