17β-Dihydroequilin | CAS 651-55-8

CAS Number: 651-55-8
Molecular Formula: C₁₈H₂₂O₂
Molecular Weight: 270.37 g/mol
Synonyms: Dihydroequilin, α-Dihydroequilin, 17α-Dihydroequilin, α-Equilol, Estra-1,3,5(10),7-tetraene-3,17-diol

Product Overview

17β-Dihydroequilin (CAS 651-55-8) is a naturally occurring estrogenic steroid belonging to the equilin family of estrogens. It is a hydrogenated derivative of equilin and is recognized as one of the important constituents associated with conjugated estrogen formulations. Due to its well-defined chemical structure and biological relevance, this compound is widely used as an analytical reference standard for pharmaceutical quality control, impurity profiling, metabolite identification, and bioanalytical research.

In pharmaceutical and biomedical laboratories, 17β-Dihydroequilin plays a significant role in supporting analytical method development using advanced chromatographic and mass spectrometric techniques. Laboratories developing methods for steroid hormones, estrogen metabolites, or conjugated estrogen drug products frequently utilize this reference material during method validation, calibration, system suitability testing, and stability studies.

Its unique steroidal structure and physicochemical characteristics make it valuable for LC-MS/MS, LC-HRMS, HPLC, and other analytical workflows requiring accurate identification and quantification of estrogenic compounds.

Applications

17β-Dihydroequilin is extensively utilized across pharmaceutical research, analytical laboratories, and regulatory testing environments.

Pharmaceutical Analysis

The compound serves as an analytical reference standard during the development and validation of analytical methods for estrogen-containing drug products. It assists scientists in achieving accurate compound identification while ensuring compliance with regulatory expectations for pharmaceutical analysis.

Bioanalytical Method Development

Researchers employ 17β-Dihydroequilin during bioanalytical assay development to evaluate extraction efficiency, chromatographic separation, matrix effects, and method sensitivity. It is suitable for quantitative assays involving plasma, serum, urine, or other biological matrices.

Metabolite Identification

As steroid metabolism studies become increasingly important during drug development, 17β-Dihydroequilin provides an essential reference for metabolite profiling using high-resolution mass spectrometry. Accurate metabolite characterization supports pharmacokinetic, toxicological, and regulatory investigations.

Pharmaceutical Quality Control

Quality control laboratories utilize authenticated reference standards to verify product identity and monitor analytical system performance. 17β-Dihydroequilin supports routine release testing, impurity analysis, and stability assessment of estrogen-based pharmaceutical formulations.

Reference Standard Applications

The compound is suitable for use as a certified or qualified laboratory reference material for:

• Analytical method validation
• Instrument calibration
• System suitability testing
• Chromatographic retention time confirmation
• Mass spectral library verification
• Reference spectrum generation
• Forced degradation studies
• Stability indicating method development

USP reference standards list 17α-Dihydroequilin for quality testing of conjugated estrogens and related pharmaceutical monographs, highlighting its relevance in compendial analysis.

Analytical Techniques

ResolveMass Laboratories supports comprehensive characterization of steroid reference compounds using modern analytical instrumentation.

Analytical techniques commonly employed include:

• LC-MS/MS
• LC-HRMS
• UHPLC
• HPLC-UV
• High-resolution accurate mass spectrometry
• Structural confirmation by MS/MS fragmentation
• Purity assessment
• Identity confirmation
• Impurity profiling
• Stability evaluation

These analytical approaches provide reliable identification while meeting the stringent quality requirements expected in pharmaceutical development.

Importance in Steroid Research

Steroid hormones represent one of the most analytically challenging classes of pharmaceutical compounds because of their structural similarities, multiple isomeric forms, and low endogenous concentrations in biological samples.

17β-Dihydroequilin contributes significantly to research involving:

• Estrogen metabolism
• Steroid biosynthesis
• Endocrine research
• Hormone replacement therapy studies
• Pharmacokinetic investigations
• Biomarker discovery
• Clinical bioanalysis
• Comparative steroid profiling

Accurate reference materials improve confidence in chromatographic identification and quantitative analysis while minimizing analytical variability.

Quality Considerations

Reference standards intended for pharmaceutical research should demonstrate high chemical purity, confirmed molecular identity, and appropriate analytical documentation.

Important quality attributes include:

• Confirmed molecular identity
• High analytical purity
• Reliable chromatographic performance
• Stable storage characteristics
• Comprehensive analytical documentation
• Batch-to-batch consistency
• Suitability for validated analytical methods

These characteristics help laboratories maintain reproducible analytical performance throughout method development and routine testing.

Role in Regulatory Studies

Regulatory agencies emphasize the use of qualified reference standards during analytical method validation and pharmaceutical quality assessment. High-quality analytical reference materials support data integrity and improve confidence during regulatory submissions.

17β-Dihydroequilin may be incorporated into studies supporting:

• Pharmaceutical development
• Method qualification
• Method validation
• Stability studies
• Bioanalytical validation
• Quality control investigations
• Regulatory documentation
• Research applications

The availability of well-characterized reference compounds facilitates compliance with current pharmaceutical analytical expectations.

Why Choose ResolveMass Laboratories?

ResolveMass Laboratories Inc. provides high-quality analytical reference standards and specialized analytical services supporting pharmaceutical, biotechnology, and academic research organizations. Our scientific expertise in mass spectrometry and pharmaceutical analysis enables reliable characterization of complex small molecules, steroid compounds, metabolites, and impurities.

Our capabilities include:

• High-resolution mass spectrometry
• LC-MS/MS method development
• Analytical method validation
• Structural elucidation
• Impurity identification
• Reference standard characterization
• Pharmaceutical quality testing
• Custom analytical solutions

Every product is supported by rigorous analytical characterization to ensure reliability, consistency, and suitability for advanced research and regulated pharmaceutical applications.

Whether supporting early-stage research, pharmaceutical quality control, bioanalytical studies, or regulatory submissions, ResolveMass Laboratories delivers scientifically robust analytical solutions that help researchers generate accurate, reproducible, and regulatory-compliant data.

 

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