4-Methyl-1H-Pyrazole-3,5-d2 | CAS 7554-65-6

OVERVIEW  of  4-Methyl-1H-Pyrazole-3,5-d2 | CAS 7554-65-6

4-Methyl-1H-pyrazole-3,5-d2 (CAS 7554-65-6) is a selectively deuterated heterocyclic compound featuring two deuterium atoms incorporated at the 3- and 5-positions of the pyrazole ring. This isotopic labeling enhances mass differentiation without altering the native chemical behavior, making it an ideal internal standard for LC-MS/MS and GC-MS workflows.
Widely used across pharmaceutical research, agrochemical residue analysis, and mechanistic chemistry, this compound supports high-precision quantitative assays and kinetic studies. Its high isotopic enrichment and purity ensure consistent analytical performance in regulated and research environments.

CHEMICAL INFORMATION

Chemical Name: 4-Methyl-1H-pyrazole-3,5-d2
Synonyms: Deuterated 4-methylpyrazole, Pyrazole-d2 derivative
CAS Number: 7554-65-6
Molecular Formula: C₄H₄D₂N₂
Molecular Weight: 84.12 g/mol
Isotopic Enrichment: ≥98 atom % D
Functional Groups: Pyrazole ring, methyl group

APPLICATIONS  of  4-Methyl-1H-Pyrazole-3,5-d2 | CAS 7554-65-6

• Isotope-Dilution Mass Spectrometry (IDMS): Reliable internal standard for quantifying pyrazoles and related compounds.

• Pharmaceutical Research: Supports metabolism studies, impurity profiling, and reaction monitoring.

• Agrochemical Analysis: Used for pesticide residue quantification and method validation.

• Environmental Testing: Suitable for trace-level detection of nitrogen-containing heterocycles.

• Kinetic & Mechanistic Studies: Deuterium incorporation aids in deciphering reaction pathways and isotope effects.

FEATURES & BENEFITS

• High Isotopic Integrity: Strong mass separation improves quantitation accuracy.

• Excellent Chemical Purity: Ensures reproducible results across analytical platforms.

• Stable Under Analytical Conditions: Maintains isotopic labeling throughout sample preparation and analysis.

• Structurally Equivalent to Parent Molecule: Offers perfect compatibility for comparative studies.

• Low Matrix Interference: Provides clean mass spectrometric profiling even in complex samples.

SUMMARY

4-Methyl-1H-pyrazole-3,5-d2 (CAS 7554-65-6) is a high-quality deuterated pyrazole derivative designed for precise quantitation in LC-MS/MS and GC-MS workflows. With excellent isotopic labeling and stability, it is widely used in pharmaceuticals, agrochemical testing, forensic toxicology, and mechanistic chemistry. Its reliability and purity make it a preferred choice for advanced analytical applications.

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