5-Hydroxyindole-2,4,6-d3-3-acetic-2,2-d2 acid

OVERVIEW  of  5-Hydroxyindole-2,4,6-d3-3-acetic-2,2-d2 acid

5-Hydroxyindole-2,4,6,7-d₃-3-acetic-2,2-d₂ acid is a multi-deuterated analog of 5-hydroxyindoleacetic acid (5-HIAA), the primary metabolite of serotonin. This compound contains three deuterium atoms on the indole ring and two on the acetic side chain, providing a total of five isotopic substitutions. These labels produce clear mass differentiation while preserving the native chemical and biological behavior of 5-HIAA.

Because of its high isotopic purity and structural equivalence to the natural metabolite, this compound is widely used in quantitative LC-MS/MS assays, clinical diagnostics, metabolomics, neurochemical pathway studies, endocrine research, and pharmaceutical development.
Manufactured with strict quality control by ResolveMass Laboratories Inc., it provides exceptional stability, accuracy, and reproducibility for advanced analytical workflows.

CHEMICAL INFORMATION

Chemical Name: 5-Hydroxyindole-2,4,6,7-d₃-3-acetic-2,2-d₂ acid

Synonyms: Deuterated 5-HIAA; 5-Hydroxyindoleacetic-d₅ acid

Isotopic Labeling: d₃ on indole ring + d₂ on acetic side chain

Molecular Formula: C₁₀H₅D₅NO₃

Functional Groups: Indole, phenolic OH, carboxylic acid, deuterated acetic group

APPLICATIONS  of  5-Hydroxyindole-2,4,6-d3-3-acetic-2,2-d2 acid\

Metabolomics & Neurochemical Pathway Studies
Used to investigate serotonin metabolism, neurotransmitter degradation pathways, and tryptophan-derived biochemical processes. Ideal for metabolic flux and pathway tracing experiments.

Quantitative LC-MS/MS Biomarker Analysis
Serves as the preferred stable-isotope internal standard for 5-HIAA quantification in:

• Plasma

• Serum

• Urine

• Cerebrospinal fluid

• Tissue extracts

Ensures high-precision quantitation in clinical and research environments.

Clinical Diagnostics
Used in validated LC-MS/MS assays for disorders including:

• Neuroendocrine tumors

• Carcinoid syndrome

• Serotonin overproduction disorders

• Endocrine biomarker monitoring

Isotope-Dilution Mass Spectrometry (IDMS)
Provides superior accuracy for regulated, trace-level quantitation in clinical and biochemical laboratories.

Pharmaceutical & Biomedical Research
Supports pharmacokinetic/pharmacodynamic (PK/PD) studies, drug–metabolite interaction experiments, and biochemical pathway investigations related to serotonin metabolism.

Biochemical & Enzymatic Studies
Useful for studying oxidative catabolism, MAO-mediated pathways, and metabolic dysregulation involving indole derivatives.

FEATURES & BENEFITS

• High Deuterium Stability: Maintains isotopic integrity during LC-MS/MS sample preparation and analysis.

• Structural Equivalence to 5-HIAA: Ensures consistent retention time, ionization efficiency, and chromatographic behavior.

• Superior Analytical Accuracy: Offers strong MS signal with minimal background interference, even in complex biological matrices.

• Versatile Research Utility: Suitable for clinical chemistry, neuroscience, metabolomics, PK studies, and diagnostic method development.

• Supports Regulatory Compliance: Ideal for validated clinical and diagnostic workflows requiring strict quantitation.

• Custom Labeling Capabilities: ResolveMass Laboratories offers tailored deuterium-labeled indole metabolites for specialized research needs.

SUMMARY

5-Hydroxyindole-2,4,6,7-d₃-3-acetic-2,2-d₂ acid is a high-purity, multi-deuterated internal standard specifically designed for precise quantitation of 5-HIAA in LC-MS/MS assays. With five deuterium atoms enabling excellent isotopic separation, it supports accurate biomarker measurement, serotonin metabolism research, neurochemical profiling, and clinical diagnostic testing. Manufactured by ResolveMass Laboratories Inc., this compound delivers exceptional isotopic enrichment, chemical stability, and analytical reliability across a wide range of advanced research and diagnostic applications.

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