Acetyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-17-0

OVERVIEW  of  Acetyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-17-0
Acetyl-L-Carnitine-d₃ HCl (N-methyl-d₃) is a stable isotope-labeled derivative of acetyl-L-carnitine, a naturally occurring quaternary ammonium compound that facilitates acetyl group transport across mitochondrial membranes. In this deuterated form, three hydrogens on the N-methyl group of the carnitine moiety are replaced by deuterium atoms, introducing a +3 Da mass shift without altering the compound’s biochemical behavior.

This isotopic substitution makes Acetyl-L-Carnitine-d₃ HCl an ideal internal standard for LC-MS/MS analyses in metabolomics, clinical biochemistry, and neurochemical studies, where it enables precise quantification of acetyl-L-carnitine and related acylcarnitines in biological samples.

CHEMICAL INFORMATION

• Name: Acetyl-L-Carnitine-d₃ hydrochloride

• Molecular Formula: C₉H₁₆D₃NO₄·HCl

• Molecular Weight: 242.71 g/mol

• CAS Number: 1334532-17-0

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated acylcarnitine; stable isotope-labeled metabolite

• Stability: Stable under inert and dry conditions

APPLICATIONS  of  Acetyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-17-0

• Targeted Metabolomics:
  Used as a stable isotope-labeled internal standard for the accurate quantification of acetyl-L-carnitine and other acylcarnitines in plasma, serum, and tissue extracts using LC-MS/MS.

• Clinical Diagnostics & Biomarker Research:
  Applied in the analysis of metabolic disorders, neurological diseases, and mitochondrial dysfunctions, where acetyl-L-carnitine levels serve as diagnostic biomarkers.

• Neurochemical Studies:
  Supports investigations into neurotransmitter metabolism, energy regulation, and acetyl group transfer processes in brain tissues.

• Mitochondrial Function & Energy Metabolism:
  Used to evaluate acetyl-CoA flux, fatty acid oxidation, and carnitine shuttle mechanisms in metabolic pathways.

• Mass Spectrometry Calibration:
  Serves as a reference compound for retention time, ion response, and mass calibration in LC-MS/MS-based assays.

ADVANTAGES  of  Acetyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-17-0

• High isotopic enrichment ensures clear mass distinction from the native compound.

• Chemically identical to the non-deuterated analog, maintaining chromatographic and ionization equivalence.

• Non-radioactive, highly stable, and safe for analytical and research use.

• Enhances data accuracy, reproducibility, and inter-laboratory comparability in quantitative assays.

HANDLING, STORAGE & SAFETY

• Handling Precautions: Use standard laboratory safety practices. Avoid prolonged exposure to air and moisture.

• Storage: Store at –20 °C in a dry, airtight container.

• Safety: Non-hazardous under normal laboratory conditions.

• Disposal: Dispose of in accordance with institutional and regional guidelines for organic laboratory chemicals.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Form: Crystalline or lyophilized solid

• Analytical Verification: Characterized by LC-MS and NMR spectroscopy

• Documentation: Supplied with Certificate of Analysis (CoA) confirming isotopic enrichment and analytical purity

SUMMARY
Acetyl-L-Carnitine-d₃ HCl (N-methyl-d₃) | CAS 1334532-17-0 is a deuterated short-chain acylcarnitine standard designed for quantitative metabolomic and biochemical analysis. It exhibits identical biological and chromatographic properties to acetyl-L-carnitine, allowing accurate measurement of endogenous concentrations in metabolic, neurological, and clinical research contexts. Its high isotopic purity and stability make it an indispensable standard for LC-MS/MS-based quantification of acylcarnitines and related metabolites in complex biological matrices.