Avanafil-¹³C-¹⁵N-D₂ | CAS No. 330784-47-9

Overview

Avanafil-¹³C-¹⁵N-D₂ is a stable isotope-labeled analogue of Avanafil, a selective phosphodiesterase type 5 (PDE5) inhibitor used in the treatment of erectile dysfunction. This isotopically labeled reference standard contains one Carbon-13 (¹³C), one Nitrogen-15 (¹⁵N), and two Deuterium (D₂) atoms, enabling precise differentiation from the native compound during mass spectrometric analysis while maintaining nearly identical chemical and chromatographic behavior.

Stable isotope-labeled compounds are considered the gold standard as internal standards in LC-MS/MS and LC-HRMS assays because they compensate for matrix effects, extraction losses, ion suppression, and instrumental variability. As a result, Avanafil-¹³C-¹⁵N-D₂ is extensively utilized in pharmaceutical research, bioanalytical method development, pharmacokinetic studies, bioequivalence studies, metabolite profiling, and regulatory analytical testing.

ResolveMass Laboratories Inc. provides high-quality stable isotope-labeled reference standards designed to support pharmaceutical companies, CROs, CDMOs, academic institutions, and analytical laboratories worldwide.

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Product Highlights

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Description

Avanafil is a second-generation PDE5 inhibitor characterized by its rapid onset of action and high selectivity. Quantitative determination of Avanafil in biological matrices such as plasma, serum, and urine requires highly accurate analytical methods due to complex sample matrices and low analyte concentrations.

Avanafil-¹³C-¹⁵N-D₂ is specifically developed to serve as an internal standard in mass spectrometry-based assays. Because the labeled compound possesses nearly identical physicochemical properties to native Avanafil, it co-elutes during chromatographic separation while being easily distinguished by its increased molecular mass.

This isotopic labeling ensures reliable correction for:

• Sample preparation losses
• Matrix effects
• Ion suppression or enhancement
• Instrument response fluctuations
• Variations in extraction recovery

The result is improved quantitative accuracy, enhanced reproducibility, and greater confidence in analytical data generated during pharmaceutical research and regulated bioanalysis.

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Key Features

• Stable isotope-labeled analogue of Avanafil
• Excellent internal standard for LC-MS/MS analysis
• Compatible with LC-HRMS workflows
• Corrects for matrix effects during quantitative analysis
• Improves assay precision and reproducibility
• Supports pharmacokinetic and bioequivalence studies
• Suitable for regulated and non-regulated bioanalysis
• Ideal for pharmaceutical analytical laboratories
• High isotopic enrichment
• Designed for research applications

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Applications

Avanafil-¹³C-¹⁵N-D₂ has broad applications throughout pharmaceutical development and analytical research.

Bioanalytical Applications

• Quantitative LC-MS/MS assays
• LC-HRMS quantitative analysis
• Bioanalytical method validation
• Clinical bioanalysis
• Therapeutic drug monitoring research
• Plasma concentration analysis
• Serum sample analysis
• Urine analysis

Pharmaceutical Research

• Pharmacokinetic (PK) studies
• Toxicokinetic studies
• Drug metabolism investigations
• Bioavailability studies
• Bioequivalence studies
• Generic drug development
• Clinical pharmacology research
• Analytical method development

Quality Control Applications

• Reference standard qualification
• Method transfer studies
• Laboratory quality assurance
• Instrument performance verification
• Analytical troubleshooting

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Benefits of Using Stable Isotope-Labeled Standards

Stable isotope-labeled internal standards significantly improve quantitative mass spectrometric analysis by minimizing analytical variability.

Major benefits include:

• Accurate quantification
• Improved method precision
• Better reproducibility
• Reduced matrix interference
• Compensation for extraction variability
• Enhanced analytical robustness
• Improved batch-to-batch consistency
• Reliable trace-level quantification
• Compliance with regulatory bioanalytical practices

These advantages make Avanafil-¹³C-¹⁵N-D₂ an essential component of validated analytical workflows.

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Role in LC-MS/MS Analysis

During LC-MS/MS analysis, the isotope-labeled internal standard is added to every calibration standard, quality control sample, and unknown sample before extraction.

The analytical workflow typically includes:

1. Addition of Avanafil-¹³C-¹⁵N-D₂ to each sample.
2. Sample preparation using protein precipitation, SPE, or liquid-liquid extraction.
3. Chromatographic separation by reversed-phase liquid chromatography.
4. Detection using tandem mass spectrometry in MRM mode.
5. Quantification using analyte-to-internal standard peak area ratios.

This workflow compensates for variations introduced during sample preparation and instrumental analysis, resulting in highly reproducible quantitative data.

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Research Areas

Avanafil-¹³C-¹⁵N-D₂ is widely employed across various research disciplines, including:

• Pharmaceutical development
• Drug metabolism studies
• Clinical pharmacology
• Bioanalytical research
• Pharmacokinetic evaluation
• Bioequivalence testing
• Analytical chemistry
• Mass spectrometry method optimization
• Metabolite identification
• Stability studies
• Regulatory submission support

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Advantages for Pharmaceutical Laboratories

Analytical laboratories benefit from the use of Avanafil-¹³C-¹⁵N-D₂ because it provides:

• Superior analytical accuracy
• Excellent chromatographic matching with native Avanafil
• Reliable correction of instrument variability
• Improved sensitivity at low concentration levels
• Reduced assay variability
• Greater confidence in quantitative results
• Consistent analytical performance
• Enhanced regulatory acceptance of validated methods

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Quality Standards

ResolveMass Laboratories Inc. supplies stable isotope-labeled reference standards manufactured to support advanced pharmaceutical research and bioanalytical testing. Each product is designed to deliver reliable analytical performance suitable for LC-MS/MS, LC-HRMS, and other mass spectrometry-based applications.

Our isotope-labeled reference materials are intended for:

• Pharmaceutical R&D
• CRO laboratories
• CDMO analytical services
• Academic research
• Contract testing laboratories
• Method development laboratories
• Regulatory bioanalysis
• Quality control laboratories

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Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. is a trusted supplier of stable isotope-labeled standards, analytical reference materials, metabolite standards, and pharmaceutical impurities for the global life sciences industry.

Our Expertise Includes

• Stable isotope-labeled reference standards
• Pharmaceutical analytical standards
• LC-MS/MS method support
• LC-HRMS applications
• Custom isotope labeling solutions
• Bioanalytical method development
• Metabolite reference standards
• Impurity standards
• Analytical consulting support
• Research-grade reference materials

Our products are developed to meet the evolving needs of pharmaceutical, biotechnology, academic, and contract research laboratories seeking high-quality reference materials for accurate and reproducible analytical results.

 

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