Benzidine-d8 | CAS 92890-63-6

OVERVIEW  of  Benzidine-d8 | CAS 92890-63-6
Benzidine-d₈ is the fully deuterated isotopic analog of benzidine, a symmetrical aromatic diamine in which all eight hydrogen atoms on the benzene rings are replaced by deuterium. This stable isotope-labeled compound maintains the same structural and electronic properties as unlabeled benzidine but with a mass increase of +8 Da, making it highly suitable for analytical, spectroscopic, and tracer studies.

Benzidine-d₈ is primarily used in mass spectrometry (MS) and nuclear magnetic resonance (NMR) as an internal standard, as well as in research involving aromatic amine reactivity, polymer chemistry, and dye intermediate analysis.

CHEMICAL INFORMATION

•  Name: Benzidine-d₈

• Molecular Formula: C₁₂D₈N₂

• Molecular Weight: 192.29 g/mol

• CAS Number: 92890-63-6

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated aromatic diamine

• Stability: Stable under inert atmosphere and dry conditions; sensitive to light and air oxidation

APPLICATIONS  of  Benzidine-d8 | CAS 92890-63-6

• Mass Spectrometry (MS) & Isotopic Labeling:
  Benzidine-d₈ is used as an internal standard or reference compound in GC-MS and LC-MS analyses to differentiate and quantify benzidine and related aromatic amines with precision through the +8 Da mass shift.

• NMR Spectroscopy:
  Useful as a deuterated reference or for solvent suppression in specialized NMR applications involving aromatic diamines.

• Mechanistic and Kinetic Studies:
  Employed to study reaction kinetics, isotopic substitution effects, and hydrogen–deuterium exchange (HDX) behavior in aromatic systems.

• Material and Polymer Research:
  Serves as a model compound for studying polymer intermediates, azo dye formation, and aromatic crosslinking processes under isotopic labeling conditions.

• Environmental and Toxicological Research:
  Utilized as a stable isotope tracer to monitor the environmental degradation or metabolic fate of aromatic amines and azo compounds.

ADVANTAGES  of  Benzidine-d8 | CAS 92890-63-6

• High isotopic enrichment (≥ 98 atom % D) for precise isotopic differentiation.

• Chemically equivalent to benzidine, ensuring identical reactivity and analytical behavior.

• Non-radioactive and environmentally safe stable isotope tracer.

• Ideal for quantitative and comparative mass spectrometric analysis.

• Enhances spectral resolution in analytical and mechanistic studies.

HANDLING, STORAGE & SAFETY

• Hazards: Benzidine and its derivatives are known carcinogens and toxic upon inhalation or skin contact.

• Precautions: Handle only in a well-ventilated fume hood with gloves, goggles, and protective clothing. Avoid all forms of exposure.

• Storage: Keep in a tightly closed, inert container under nitrogen or argon, stored at 2–8 °C, protected from light and air.

• Disposal: Dispose of according to hazardous waste regulations for aromatic amines.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Analytical Verification: Confirmed by ¹H NMR (absence of proton peaks), ²H NMR, LC-MS, and IR spectroscopy

• Appearance: Off-white crystalline solid

• Documentation: Certificate of Analysis (CoA) provided with isotopic and purity data

SUMMARY
Benzidine-d₈ (CAS 92890-63-6) is a deuterium-labeled aromatic diamine used extensively in mass spectrometry, NMR spectroscopy, and mechanistic studies of aromatic compounds. Its high isotopic enrichment and chemical stability make it a preferred reagent for quantitative analysis, isotope effect investigations, and synthetic research. With careful handling due to its toxicity, benzidine-d₈ serves as a vital tool in both analytical and industrial chemistry for precise isotopic applications.