Butyryl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-21-6

OVERVIEW  of  Butyryl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-21-6
Butyryl-L-Carnitine-d₃ HCl (N-methyl-d₃) is a stable isotope-labeled compound derived from butyryl-L-carnitine, a short-chain acylcarnitine involved in the transport of fatty acids into mitochondria for β-oxidation and energy metabolism. In this labeled analog, three hydrogen atoms on the N-methyl group of the L-carnitine moiety are replaced with deuterium atoms, creating a +3 Da molecular mass shift while maintaining identical chemical and biological properties to the native compound.

This deuterated standard is widely used in quantitative metabolomics, lipidomics, and clinical diagnostics as an internal standard for LC-MS/MS analysis of acylcarnitines in biological matrices. It enables accurate quantitation and correction of matrix effects, ensuring reproducibility and precision in analytical workflows.

CHEMICAL INFORMATION

• Name: Butyryl-L-Carnitine-d₃ hydrochloride

• Molecular Formula: C₁₁H₁₉D₃NO₄·HCl

• Molecular Weight: 270.77 g/mol

• CAS Number: 1334532-21-6

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated acylcarnitine; stable isotope-labeled metabolite

• Stability: Chemically stable under inert, dry conditions; hygroscopic when exposed to air

APPLICATIONS  of  Butyryl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-21-6

• Targeted Metabolomics & Lipidomics:
  Serves as a stable isotope-labeled internal standard for the quantification of butyryl-L-carnitine and related acylcarnitines in plasma, serum, and tissue using LC-MS/MS.

• Clinical & Diagnostic Studies:
  Utilized in acylcarnitine profiling to identify abnormalities in fatty acid oxidation disorders (FAODs) and mitochondrial dysfunctions in metabolic screening assays.

• Biochemical & Energy Metabolism Research:
  Enables detailed investigation of short-chain fatty acid metabolism, mitochondrial transport, and β-oxidation pathways in biological systems.

• Analytical Calibration & Quality Control:
  Used as a mass spectrometry calibration standard and QC compound to validate instrument performance, retention time, and response linearity.

• Quantitative Bioanalysis:
  Ensures reliable quantitation by compensating for sample matrix variability and ion suppression in LC-MS/MS workflows.

ADVANTAGES  of  Butyryl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 1334532-21-6

• High isotopic enrichment (≥ 98 atom % D) provides clear mass spectral differentiation from the native form.

• Identical chromatographic and ionization characteristics ensure consistent analytical behavior.

• Non-radioactive, chemically stable, and safe for routine use.

• Enhances reproducibility and precision in metabolomic and clinical assays.

HANDLING, STORAGE & SAFETY

• Handling Precautions: Handle using standard laboratory PPE (gloves, goggles, lab coat). Avoid prolonged exposure to air and moisture.

• Storage: Store at –20 °C in a dry, airtight container; protect from light and humidity.

• Safety: Non-toxic under analytical use; not classified as hazardous.

• Disposal: Dispose according to institutional and regulatory guidelines for non-hazardous organic materials.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Form: Crystalline or amorphous solid

• Analytical Verification: Confirmed by LC-MS/MS and ¹H/²H NMR spectroscopy

• Documentation: Supplied with Certificate of Analysis (CoA) verifying isotopic purity and analytical confirmation

SUMMARY
Butyryl-L-Carnitine-d₃ HCl (N-methyl-d₃) | CAS 1334532-21-6 is a deuterium-labeled short-chain acylcarnitine designed for quantitative LC-MS/MS applications in metabolomics and clinical biochemistry. It serves as a reliable internal standard for the determination of butyryl-L-carnitine concentrations in biological matrices. With high isotopic enrichment, excellent stability, and structural equivalence to its unlabeled counterpart, this compound enhances accuracy, reproducibility, and analytical precision in studies of fatty acid oxidation, energy metabolism, and mitochondrial function.