Choline-1,1,2,2-d4 chloride | CAS 285979-70-6

Choline-1,1,2,2-d4 chloride (CAS 285979-70-6)

Overview
Choline-1,1,2,2-d4 chloride | CAS 285979-70-6 is a deuterium-labeled analogue of choline chloride designed for use as an internal standard and tracer in biochemical, metabolomic, and analytical workflows. By replacing four specific hydrogen atoms with deuterium (D), this isotopically enriched form of choline provides a predictable mass-shift in mass spectrometry (MS) and nuclear magnetic resonance (NMR) experiments while preserving the native chemical behavior of unlabeled choline. ResolveMass Laboratories supplies this reagent with rigorous quality control, backed by a Certificate of Analysis (CoA) and batch-specific data to support reproducible research and regulated laboratory workflows.

Key Features
• CAS number: 285979-70-6.
• Isotopologue: Choline-1,1,2,2-d4 chloride (deuterated at four positions).
• Typical isotopic enrichment: >98% (batch-dependent; see CoA).
• Typical chemical purity: >98% (GC/LC and NMR verified; see CoA).
• Packaging options: small-research vials to bulk quantities—custom packaging available.

Applications of Choline-1,1,2,2-d4 chloride | CAS 285979-70-6
• Internal standard for LC-MS/MS and GC-MS quantitation of choline, phosphocholine, glycerophosphocholines, and related metabolites.
• Metabolomics and lipidomics studies: accurate isotope-dilution quantification in complex biological matrices (plasma, serum, urine, tissue homogenates, cell extracts).
• Tracer studies of choline metabolism in biochemical and cell-culture experiments where tracking carbon/nitrogen fate is not required but mass-resolved tracing is desired.
• Method development and validation for clinical and research assays that quantify choline-related biomarkers.
• NMR studies where deuterium labeling reduces proton signals and helps simplify spectral interpretation.

Analytical & Quality Information
ResolveMass provides Choline-1,1,2,2-d4 chloride with thorough quality documentation. Each lot is supplied with a Certificate of Analysis that typically includes:
• Isotopic enrichment (determined by NMR or MS).
• Chemical purity (HPLC/LC, GC, or NMR).
• Water content (Karl Fischer, if applicable).
• Identity confirmation (NMR and/or HRMS).
• Batch number and recommended storage/handling notes.

Typical specification (representative; check CoA for lot-specific data):
• Isotopic enrichment: >98% D-incorporation.
• Chemical purity: >98% by HPLC or NMR.
• Appearance: White to off-white crystalline solid.

Handling & Stability
• Moisture sensitivity: The chloride salt can be hygroscopic; minimize exposure to air and humidity. Use desiccant and inert atmosphere handling for long-term storage if necessary.
• Light sensitivity: Not generally light-sensitive, but store in a tightly closed container away from direct sunlight to preserve integrity.
• Safety: Use standard laboratory PPE (gloves, eye protection, lab coat). Avoid inhalation, ingestion, or prolonged skin contact. Consult the Safety Data Sheet (SDS) provided by ResolveMass for detailed hazard, first-aid, and disposal information.

Preparation & Use Recommendations
• Internal standard preparation: Prepare aqueous stock solutions at appropriate concentrations for your assay (e.g., µM–mM range) and store aliquots at -20 °C to avoid freeze–thaw cycles.
• Matrix matching: For quantitative MS, spike the internal standard into samples early in the processing workflow (pre-extraction) to correct for extraction efficiency and matrix effects.
• Calibration & method validation: Use in isotope-dilution calibration curves to achieve high accuracy and precision; follow regulatory guidance for method validation when used in clinical or regulated settings.

Packaging & Ordering
ResolveMass offers Choline-1,1,2,2-d4 chloride in a range of sizes for research and commercial use, from small vials (mg) to larger quantities (g). Custom packaging and labeling for GLP/GMP workflows are available. All shipments include batch-specific documentation (CoA and SDS).

Regulatory & Shipping Information
This product is intended for laboratory research use only and is not for human or veterinary use. Shipping classification and restrictions depend on quantity and destination—ResolveMass handles export documentation and ensures compliance with applicable regulations. Contact our customer service team for quotes, export controls, or bulk order coordination.

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