Colchicine-d6 | CAS 1217651-73-4

Colchicine-d6 (CAS 1217651-73-4) is a stable isotope-labeled analog of colchicine in which six hydrogen atoms are replaced with deuterium (²H). This isotopically enriched reference standard is extensively used as an internal standard for LC-MS/MS, LC-HRMS, and GC-MS quantitative analysis of colchicine in pharmaceutical formulations, biological matrices, toxicology investigations, pharmacokinetic studies, and bioanalytical method validation. Because the deuterium-labeled compound exhibits nearly identical chemical and chromatographic behavior to native colchicine while remaining distinguishable by mass spectrometry, Colchicine-d6 provides exceptional analytical accuracy by compensating for sample preparation losses, matrix effects, and instrument variability.

At ResolveMass Laboratories Inc., high-quality stable isotope-labeled reference standards support regulated bioanalytical studies, pharmaceutical research, forensic testing, and drug metabolism investigations. Colchicine-d6 is particularly valuable for laboratories developing sensitive and robust quantitative assays that comply with FDA and ICH bioanalytical method validation expectations.

Product Overview

Values may vary slightly depending on manufacturer specifications.

Applications of Colchicine-d6

Colchicine-d6 serves as an indispensable analytical reference material across pharmaceutical research and regulated laboratory environments.

LC-MS/MS Quantification

The primary application of Colchicine-d6 is as an internal standard for quantitative determination of colchicine in plasma, serum, urine, whole blood, and tissue samples. Since both compounds behave similarly during extraction, chromatography, and ionization, the isotope-labeled analog effectively corrects analytical variability, leading to highly reproducible quantitative results.

Bioanalytical Method Development

Bioanalytical laboratories utilize Colchicine-d6 during method development and validation to establish:

• Accuracy
• Precision
• Recovery
• Matrix effect evaluation
• Dilution integrity
• Stability assessment
• Calibration curve performance

Its use enhances assay robustness and supports regulatory-compliant bioanalytical workflows.

Pharmacokinetic (PK) Studies

During pharmacokinetic and toxicokinetic investigations, Colchicine-d6 enables precise measurement of circulating colchicine concentrations over time. Accurate PK profiling is essential for determining absorption, distribution, metabolism, elimination, and dose proportionality.

Therapeutic Drug Monitoring

Although colchicine possesses a relatively narrow therapeutic window, quantitative LC-MS/MS methods employing Colchicine-d6 help clinical and research laboratories achieve reliable concentration measurements necessary for therapeutic monitoring and pharmacological investigations.

Toxicology and Forensic Analysis

Colchicine poisoning can result from accidental overdose or intentional exposure. Highly sensitive LC-MS/MS assays utilizing Colchicine-d6 improve confidence in forensic and toxicological investigations by minimizing analytical variability while providing reliable quantitative results.

Advantages of Using Colchicine-d6

Stable isotope-labeled internal standards are considered the gold standard for quantitative mass spectrometry because they closely mimic the physicochemical properties of the target analyte.

Key advantages include:

• Excellent correction for ion suppression and matrix effects
• Improved quantitative accuracy
• Higher analytical precision
• Compensation for extraction variability
• Enhanced reproducibility between analytical batches
• Better long-term instrument performance monitoring
• Increased confidence in regulated bioanalytical studies
• Suitable for ultra-trace quantitative analysis

Because the isotope label alters only the molecular mass without significantly affecting chromatographic retention or extraction efficiency, Colchicine-d6 provides reliable normalization throughout the analytical workflow.

Compatible Analytical Techniques

Colchicine-d6 is compatible with numerous analytical platforms, including:

• LC-MS/MS
• UHPLC-MS/MS
• LC-HRMS
• Triple Quadrupole Mass Spectrometry
• Orbitrap HRMS
• QTOF LC-MS
• GC-MS (where applicable)
• UPLC coupled with tandem mass spectrometry

These techniques are routinely employed in pharmaceutical research, CRO laboratories, academic institutions, and regulatory testing facilities.

Pharmaceutical Research Applications

Researchers use Colchicine-d6 throughout multiple stages of drug development, including:

• Bioavailability studies
• Bioequivalence studies
• Clinical pharmacology
• Drug-drug interaction studies
• Metabolism investigations
• Toxicokinetic studies
• Preclinical safety studies
• Clinical sample analysis
• Method transfer between laboratories

Its consistent analytical performance makes it particularly valuable during regulated GLP and GCP studies requiring highly reproducible quantitative data.

Why Choose Stable Isotope-Labeled Standards?

Stable isotope-labeled standards have become essential components of modern quantitative mass spectrometry. Compared with external calibration alone, isotope dilution techniques significantly reduce analytical uncertainty by correcting for losses that occur during:

• Sample preparation
• Protein precipitation
• Solid-phase extraction
• Liquid-liquid extraction
• Chromatographic separation
• Electrospray ionization
• Instrument response fluctuations

As a result, isotope-labeled internal standards such as Colchicine-d6 improve assay robustness and facilitate compliance with stringent regulatory expectations for quantitative bioanalysis.

 

Learn more about Deuterated Chemicals through,

• Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends
• Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 
• ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States.
• Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 
• How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 
• How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 
• Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 
• Custom Synthesis of Deuterated Chemicals