Deuterated α-Hydroxyisobutyric Acid-d6 (CAS 40662-45-1)

Deuterated α-Hydroxyisobutyric acid-d6 (2-Hydroxyisobutyric acid-d6, CAS 40662-45-1) is a stable isotope-labeled analog of α-hydroxyisobutyric acid in which six hydrogen atoms are replaced with deuterium (²H). This isotopically enriched compound is widely utilized in analytical chemistry, metabolomics, pharmaceutical research, and bioanalytical method development as an internal standard for accurate quantitative analysis by LC-MS/MS, GC-MS, and NMR spectroscopy. Because the deuterated analog exhibits nearly identical chemical and chromatographic behavior to the native molecule while remaining distinguishable by its mass, it enables highly reliable quantification with improved precision and reduced analytical variability.

At ResolveMass Laboratories Inc., Deuterated α-Hydroxyisobutyric acid-d6 is supplied exclusively for research and laboratory applications. It is intended to support scientists involved in biomarker research, metabolite profiling, pharmaceutical analysis, clinical research, and stable isotope dilution assays requiring high analytical confidence.

Product Overview

Deuterated α-Hydroxyisobutyric acid-d6 is chemically identical to the unlabeled compound except for the substitution of six hydrogen atoms with deuterium. This isotopic substitution produces a predictable mass shift without significantly altering the molecule’s physical or chemical properties. As a result, both labeled and unlabeled compounds co-elute during chromatographic separation while remaining distinguishable by mass spectrometry.

The compound is particularly valuable in quantitative workflows because it compensates for sample preparation losses, extraction variability, ion suppression, and instrument fluctuations. Stable isotope dilution methods employing deuterated standards are considered among the most accurate quantitative approaches in modern analytical laboratories.

Chemical Information

Product Name: Deuterated α-Hydroxyisobutyric acid-d6

Synonyms:

• 2-Hydroxyisobutyric acid-d6
• 2-Hydroxy-2-methylpropanoic acid-d6
• 2-HIBA-d6

CAS Number: 40662-45-1

Molecular Formula: C₄H₂D₆O₃

Molecular Weight: 110.14 g/mol

Appearance: Off-white to light yellow solid

Isotopic Enrichment: Typically ≥98–99% deuterium incorporation (supplier dependent)

Research Use: For laboratory and research applications only. Not intended for human or veterinary use.

Applications:

LC-MS/MS Internal Standard

One of the primary applications of Deuterated α-Hydroxyisobutyric acid-d6 is its use as an internal standard for quantitative LC-MS/MS assays. Because the labeled compound behaves almost identically to endogenous α-hydroxyisobutyric acid during sample preparation and chromatographic separation, it enables precise correction for experimental variability and enhances analytical reproducibility.

GC-MS Quantification

Researchers frequently employ the compound in gas chromatography-mass spectrometry methods for quantitative metabolite analysis. Stable isotope labeling improves measurement accuracy by correcting for extraction efficiency, derivatization variability, and instrument response.

Metabolomics Research

α-Hydroxyisobutyric acid is an endogenous metabolite found in biological systems. The deuterated analog is widely used in targeted metabolomics studies to accurately quantify metabolite concentrations across plasma, serum, urine, tissue, and other biological matrices. Stable isotope standards significantly improve confidence in metabolomic data by minimizing analytical bias.

Pharmaceutical Research

Drug discovery and pharmaceutical development frequently require validated quantitative analytical methods. Deuterated α-Hydroxyisobutyric acid-d6 supports:

• Method development
• Bioanalytical validation
• Pharmacokinetic studies
• Biomarker analysis
• Metabolite quantification
• Quality control testing

Clinical Research

Clinical research laboratories utilize isotope-labeled standards to improve assay precision when measuring endogenous metabolites. Stable isotope dilution techniques remain the preferred approach for quantitative bioanalysis due to their excellent reproducibility and reduced matrix effects.

Advantages of Using Deuterated Standards

Stable isotope-labeled compounds offer several analytical advantages over external calibration methods, including:

• Improved quantitative accuracy
• Compensation for sample preparation losses
• Correction for matrix effects and ion suppression
• Enhanced precision and reproducibility
• Excellent chromatographic co-elution with the native analyte
• Reliable correction for instrument variability
• Greater confidence in regulated analytical workflows

These benefits make Deuterated α-Hydroxyisobutyric acid-d6 an essential reference material for laboratories developing highly sensitive quantitative assays.

Typical Research Areas

Researchers use this isotope-labeled compound in numerous scientific disciplines, including:

• Pharmaceutical analysis
• Bioanalytical chemistry
• Clinical metabolomics
• Biomarker discovery
• Analytical chemistry
• Drug metabolism studies
• Toxicology research
• Stable isotope dilution assays
• LC-MS/MS method validation
• Quality assurance and quality control

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. is committed to supplying high-quality stable isotope-labeled reference standards that support advanced analytical research. Our isotope-labeled compounds are designed to meet the demanding requirements of pharmaceutical companies, CROs, academic institutions, biotechnology organizations, and analytical testing laboratories.

Our products are suitable for:

• Analytical method development
• Quantitative LC-MS/MS analysis
• Stable isotope dilution assays
• Bioanalytical research
• Pharmaceutical quality control
• Clinical research applications
• Metabolomics workflows

With a focus on quality, consistency, and scientific reliability, ResolveMass Laboratories Inc. provides researchers with dependable reference materials that help achieve accurate, reproducible, and robust analytical results.

Disclaimer: This product is intended for research use only (RUO) and is not intended for human or veterinary therapeutic, diagnostic, or clinical use.

Learn more about Deuterated Chemicals through,

• Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends
• Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 
• ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States.
• Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 
• How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 
• How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 
• Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 
• Custom Synthesis of Deuterated Chemicals