Deuterated 2-Aminopyridine-d6 | CAS 203784-57-0

Deuterated 2-Aminopyridine-d6 | CAS 203784-57-0

Deuterated 2-Aminopyridine-d6 | CAS 203784-57-0 is a high-purity stable isotope-labelled analogue of 2-aminopyridine in which all six hydrogen atoms are replaced with deuterium (²H). This isotopically enriched compound is widely used as an analytical reference standard and internal standard in LC-MS/MS, GC-MS, and isotope dilution mass spectrometry (IDMS). With a molecular formula of C₅D₆N₂ and a molecular weight of approximately 100.15 g/mol, it provides a +6 Da mass shift compared to native 2-aminopyridine, enabling precise differentiation during mass spectrometric analysis while maintaining nearly identical chemical behavior to the unlabeled compound.

At ResolveMass Laboratories Inc., we supply premium-quality deuterated reference standards that support pharmaceutical research, analytical method development, bioanalysis, environmental testing, forensic investigations, and academic research. Our deuterated compounds are manufactured to stringent quality specifications to deliver consistent analytical performance across demanding laboratory workflows.

What is Deuterated 2-Aminopyridine-d6?

2-Aminopyridine is an important heterocyclic aromatic amine used extensively in medicinal chemistry, pharmaceutical synthesis, agrochemical research, and chemical manufacturing. The deuterated analogue, 2-Aminopyridine-d6, possesses identical chemical properties while exhibiting a distinct molecular mass due to the substitution of hydrogen atoms with deuterium.

Because deuterium behaves chemically almost identically to hydrogen, the labelled compound co-elutes with the native analyte during chromatographic separation. However, the mass difference allows modern mass spectrometers to distinguish the internal standard from the analyte with exceptional accuracy. This makes 2-Aminopyridine-d6 an ideal internal standard for quantitative analysis where high precision and reproducibility are essential.

Key Features

• Stable isotope-labelled analytical reference standard
• CAS Number: 203784-57-0
• Molecular Formula: C₅D₆N₂
• Molecular Weight: 100.15 g/mol
• Typically available with ≥98 atom % deuterium enrichment
• High chemical purity suitable for analytical applications
• Excellent isotopic stability
• Compatible with LC-MS/MS, GC-MS, HRMS, and NMR studies
• Chemically equivalent to native 2-aminopyridine while remaining easily distinguishable by mass spectrometry

Applications of Deuterated 2-Aminopyridine-d6

LC-MS/MS Internal Standard

One of the primary applications of 2-Aminopyridine-d6 is as an internal standard for quantitative LC-MS/MS assays. During sample preparation, extraction efficiency, matrix effects, and ionization variability can significantly affect analytical accuracy. Using an isotopically labelled analogue compensates for these variations because both compounds behave identically throughout the analytical process.

Analysts can therefore obtain highly reliable quantitative data with improved precision and reproducibility.

Pharmaceutical Research

Pharmaceutical laboratories frequently utilize deuterated standards during:

• Drug impurity analysis
• API characterization
• Stability studies
• Method validation
• Forced degradation studies
• Process development
• Quality control testing

The use of isotope-labelled compounds improves confidence in regulatory submissions while enhancing method robustness.

Bioanalytical Studies

Bioanalytical laboratories use 2-Aminopyridine-d6 during pharmacokinetic and toxicokinetic studies where accurate quantification of trace analytes is required in biological matrices such as plasma, serum, urine, or tissue extracts.

Stable isotope-labelled internal standards help minimize analytical variability introduced during extraction, sample cleanup, and ionization.

Metabolomics and Biomarker Research

Researchers performing metabolomic investigations often require isotope-labelled compounds for accurate metabolite quantification. 2-Aminopyridine derivatives are commonly employed in carbohydrate derivatization and glycan analysis, making deuterated analogues valuable tools for quantitative workflows.

Chemical Research

Academic researchers employ 2-Aminopyridine-d6 for:

• Reaction mechanism studies
• Isotope tracing experiments
• Spectroscopic investigations
• NMR simplification
• Synthetic chemistry
• Catalytic process evaluation

Because deuterium produces different spectroscopic behavior than protium, labelled compounds provide valuable mechanistic insights.

Benefits of Stable Isotope Labelled Standards

Stable isotope-labelled reference materials have become indispensable in modern analytical laboratories. Compared to structurally unrelated internal standards, deuterated compounds offer several significant advantages:

Improved Quantitative Accuracy

Since the labelled compound closely mimics the analyte, it corrects for losses occurring during extraction, cleanup, and sample preparation.

Reduced Matrix Effects

Biological and environmental samples often contain interfering compounds that suppress or enhance ionization. Stable isotope-labelled standards compensate for these effects, improving data reliability.

Excellent Chromatographic Matching

2-Aminopyridine-d6 co-elutes with the native analyte under identical chromatographic conditions, ensuring nearly identical retention behavior.

Enhanced Regulatory Compliance

Many validated analytical methods developed according to FDA, EMA, and ICH recommendations utilize isotope-labelled internal standards to achieve superior analytical performance.

Analytical Techniques

Deuterated 2-Aminopyridine-d6 is compatible with numerous analytical platforms, including:

• Liquid Chromatography-Mass Spectrometry (LC-MS)
• LC-MS/MS
• Ultra High Performance Liquid Chromatography (UHPLC-MS)
• Gas Chromatography-Mass Spectrometry (GC-MS)
• High Resolution Mass Spectrometry (HRMS)
• Tandem Mass Spectrometry
• Nuclear Magnetic Resonance (NMR)
• Isotope Dilution Mass Spectrometry (IDMS)

Its versatility makes it suitable for both research laboratories and regulated analytical environments.

Storage Recommendations

To maintain long-term stability and isotopic integrity, 2-Aminopyridine-d6 should be stored according to the supplier’s recommended conditions in tightly sealed containers, protected from excessive moisture, contamination, and prolonged exposure to light. Good laboratory handling practices help preserve chemical purity and analytical performance throughout the product’s shelf life.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. supplies premium-quality deuterated reference standards designed to meet the evolving needs of pharmaceutical, biotechnology, environmental, and academic laboratories. Our isotope-labelled compounds are selected to support high-performance analytical methods requiring exceptional precision, reproducibility, and regulatory confidence.

Researchers rely on ResolveMass for:

• High-purity stable isotope-labelled compounds
• Reliable analytical reference materials
• Consistent batch-to-batch quality
• Products suitable for method development and validation
• Support for pharmaceutical and bioanalytical research
• Responsive technical assistance and dependable global supply

Whether you are developing a new LC-MS/MS method, validating a regulated analytical procedure, or conducting advanced research involving isotope dilution techniques, Deuterated 2-Aminopyridine-d6 (CAS 203784-57-0) provides the analytical accuracy and consistency required for reliable quantitative measurements. Its excellent isotopic enrichment, chemical equivalence to the native analyte, and compatibility with modern mass spectrometry platforms make it an essential reference standard for today’s analytical laboratories.

is a high-purity stable isotope-labelled analogue of 2-aminopyridine in which all six hydrogen atoms are replaced with deuterium (²H). This isotopically enriched compound is widely used as an analytical reference standard and internal standard in LC-MS/MS, GC-MS, and isotope dilution mass spectrometry (IDMS). With a molecular formula of C₅D₆N₂ and a molecular weight of approximately 100.15 g/mol, it provides a +6 Da mass shift compared to native 2-aminopyridine, enabling precise differentiation during mass spectrometric analysis while maintaining nearly identical chemical behavior to the unlabeled compound.

At ResolveMass Laboratories Inc., we supply premium-quality deuterated reference standards that support pharmaceutical research, analytical method development, bioanalysis, environmental testing, forensic investigations, and academic research. Our deuterated compounds are manufactured to stringent quality specifications to deliver consistent analytical performance across demanding laboratory workflows.

What is Deuterated 2-Aminopyridine-d6?

2-Aminopyridine is an important heterocyclic aromatic amine used extensively in medicinal chemistry, pharmaceutical synthesis, agrochemical research, and chemical manufacturing. The deuterated analogue, 2-Aminopyridine-d6, possesses identical chemical properties while exhibiting a distinct molecular mass due to the substitution of hydrogen atoms with deuterium.

Because deuterium behaves chemically almost identically to hydrogen, the labelled compound co-elutes with the native analyte during chromatographic separation. However, the mass difference allows modern mass spectrometers to distinguish the internal standard from the analyte with exceptional accuracy. This makes 2-Aminopyridine-d6 an ideal internal standard for quantitative analysis where high precision and reproducibility are essential.

Key Features

• Stable isotope-labelled analytical reference standard
• CAS Number: 203784-57-0
• Molecular Formula: C₅D₆N₂
• Molecular Weight: 100.15 g/mol
• Typically available with ≥98 atom % deuterium enrichment
• High chemical purity suitable for analytical applications
• Excellent isotopic stability
• Compatible with LC-MS/MS, GC-MS, HRMS, and NMR studies
• Chemically equivalent to native 2-aminopyridine while remaining easily distinguishable by mass spectrometry

Applications of Deuterated 2-Aminopyridine-d6

LC-MS/MS Internal Standard

One of the primary applications of 2-Aminopyridine-d6 is as an internal standard for quantitative LC-MS/MS assays. During sample preparation, extraction efficiency, matrix effects, and ionization variability can significantly affect analytical accuracy. Using an isotopically labelled analogue compensates for these variations because both compounds behave identically throughout the analytical process.

Analysts can therefore obtain highly reliable quantitative data with improved precision and reproducibility.

Pharmaceutical Research

Pharmaceutical laboratories frequently utilize deuterated standards during:

• Drug impurity analysis
• API characterization
• Stability studies
• Method validation
• Forced degradation studies
• Process development
• Quality control testing

The use of isotope-labelled compounds improves confidence in regulatory submissions while enhancing method robustness.

Bioanalytical Studies

Bioanalytical laboratories use 2-Aminopyridine-d6 during pharmacokinetic and toxicokinetic studies where accurate quantification of trace analytes is required in biological matrices such as plasma, serum, urine, or tissue extracts.

Stable isotope-labelled internal standards help minimize analytical variability introduced during extraction, sample cleanup, and ionization.

Metabolomics and Biomarker Research

Researchers performing metabolomic investigations often require isotope-labelled compounds for accurate metabolite quantification. 2-Aminopyridine derivatives are commonly employed in carbohydrate derivatization and glycan analysis, making deuterated analogues valuable tools for quantitative workflows.

Chemical Research

Academic researchers employ 2-Aminopyridine-d6 for:

• Reaction mechanism studies
• Isotope tracing experiments
• Spectroscopic investigations
• NMR simplification
• Synthetic chemistry
• Catalytic process evaluation

Because deuterium produces different spectroscopic behavior than protium, labelled compounds provide valuable mechanistic insights.

Benefits of Stable Isotope Labelled Standards

Stable isotope-labelled reference materials have become indispensable in modern analytical laboratories. Compared to structurally unrelated internal standards, deuterated compounds offer several significant advantages:

Improved Quantitative Accuracy

Since the labelled compound closely mimics the analyte, it corrects for losses occurring during extraction, cleanup, and sample preparation.

Reduced Matrix Effects

Biological and environmental samples often contain interfering compounds that suppress or enhance ionization. Stable isotope-labelled standards compensate for these effects, improving data reliability.

Excellent Chromatographic Matching

2-Aminopyridine-d6 co-elutes with the native analyte under identical chromatographic conditions, ensuring nearly identical retention behavior.

Enhanced Regulatory Compliance

Many validated analytical methods developed according to FDA, EMA, and ICH recommendations utilize isotope-labelled internal standards to achieve superior analytical performance.

Analytical Techniques

Deuterated 2-Aminopyridine-d6 is compatible with numerous analytical platforms, including:

• Liquid Chromatography-Mass Spectrometry (LC-MS)
• LC-MS/MS
• Ultra High Performance Liquid Chromatography (UHPLC-MS)
• Gas Chromatography-Mass Spectrometry (GC-MS)
• High Resolution Mass Spectrometry (HRMS)
• Tandem Mass Spectrometry
• Nuclear Magnetic Resonance (NMR)
• Isotope Dilution Mass Spectrometry (IDMS)

Its versatility makes it suitable for both research laboratories and regulated analytical environments.

Storage Recommendations

To maintain long-term stability and isotopic integrity, 2-Aminopyridine-d6 should be stored according to the supplier’s recommended conditions in tightly sealed containers, protected from excessive moisture, contamination, and prolonged exposure to light. Good laboratory handling practices help preserve chemical purity and analytical performance throughout the product’s shelf life.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. supplies premium-quality deuterated reference standards designed to meet the evolving needs of pharmaceutical, biotechnology, environmental, and academic laboratories. Our isotope-labelled compounds are selected to support high-performance analytical methods requiring exceptional precision, reproducibility, and regulatory confidence.

Researchers rely on ResolveMass for:

• High-purity stable isotope-labelled compounds
• Reliable analytical reference materials
• Consistent batch-to-batch quality
• Products suitable for method development and validation
• Support for pharmaceutical and bioanalytical research
• Responsive technical assistance and dependable global supply

Whether you are developing a new LC-MS/MS method, validating a regulated analytical procedure, or conducting advanced research involving isotope dilution techniques, Deuterated 2-Aminopyridine-d6 (CAS 203784-57-0) provides the analytical accuracy and consistency required for reliable quantitative measurements. Its excellent isotopic enrichment, chemical equivalence to the native analyte, and compatibility with modern mass spectrometry platforms make it an essential reference standard for today’s analytical laboratories.

Learn more about Deuterated Chemiclas through,

• Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends
• Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 
• ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States.
• Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 
• How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 
• How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 
• Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 
• Custom Synthesis of Deuterated Chemicals