Deuterated Terephthalic-2,3,5,6-d4 Acid | CAS 60088-54-2

Deuterated Terephthalic-2,3,5,6-d4 Acid (CAS 60088-54-2) is a stable isotope-labeled analogue of terephthalic acid in which the four aromatic hydrogen atoms on the benzene ring are replaced with deuterium (²H). This isotopic substitution provides a predictable M+4 mass shift while preserving the compound’s physicochemical properties, making it an ideal internal standard and reference material for quantitative analytical applications. The compound is widely employed in pharmaceutical analysis, polymer characterization, environmental monitoring, isotope dilution mass spectrometry (IDMS), and analytical method validation. Typical commercial material is supplied with approximately 98 atom% deuterium enrichment and has the molecular formula C8H2D4O4 with a molecular weight of 170.16 g/mol.

As industries continue to demand increasingly sensitive analytical methods, isotope-labeled standards have become indispensable for improving quantification accuracy. Deuterated Terephthalic Acid closely mimics the chromatographic behavior, extraction efficiency, and ionization characteristics of native terephthalic acid while remaining easily distinguishable by mass spectrometry. This unique combination significantly reduces matrix effects, minimizes analytical variability, and improves confidence in trace-level measurements across complex sample matrices.

Applications of Deuterated Terephthalic-2,3,5,6-d4 Acid

Internal Standard for LC-MS and GC-MS

One of the primary applications of Deuterated Terephthalic Acid is as an internal standard in LC-MS and GC-MS workflows. Since it behaves almost identically to unlabeled terephthalic acid during sample preparation and chromatographic separation, it enables accurate correction for extraction losses, ion suppression, and instrument variability. Laboratories performing quantitative analysis rely on this isotope-labeled compound to generate highly reproducible analytical data.

Polymer and PET Material Analysis

Terephthalic acid is one of the principal monomers used in the manufacture of polyethylene terephthalate (PET) and numerous polyester materials. Deuterated Terephthalic Acid is extensively utilized during polymer research to investigate degradation pathways, monitor residual monomers, evaluate hydrolysis products, and assess product purity. It is particularly valuable during quality control of PET resins, packaging materials, textile fibers, engineering plastics, and recycled polymer products.

Isotope Dilution Mass Spectrometry (IDMS)

The compound serves as an ideal isotopic tracer for isotope dilution mass spectrometry, one of the most accurate quantitative analytical techniques available. Because the labeled and unlabeled molecules exhibit nearly identical chemical behavior, IDMS provides highly reliable absolute quantification with excellent precision and traceability. This approach is widely adopted in pharmaceutical laboratories, regulatory testing, environmental research, and certified reference material development.

Environmental Monitoring

Increasing environmental concern regarding plastic waste and PET degradation has expanded the need for accurate monitoring of terephthalic acid in water, soil, wastewater, landfill leachates, and industrial effluents. Deuterated Terephthalic Acid enables precise quantification of trace contaminants while compensating for sample preparation losses and complex environmental matrices. It is also useful for studying degradation products generated during plastic recycling and environmental weathering.

Method Development and Validation

Analytical laboratories use Deuterated Terephthalic Acid during method development and validation to establish calibration curves, evaluate extraction recovery, determine precision and accuracy, assess matrix effects, and verify long-term method robustness. The isotope-labeled analogue supports compliance with regulatory expectations for validated quantitative analytical methods.

Key Advantages

Deuterated Terephthalic-2,3,5,6-d4 Acid offers several analytical advantages that make it an essential tool for modern laboratories:

• Stable four-deuterium labeling provides a clear and reproducible M+4 mass difference for confident identification by mass spectrometry.
• Chemically equivalent to native terephthalic acid, ensuring comparable chromatographic retention and extraction performance.
• High isotopic enrichment minimizes interference from unlabeled species and improves quantitative reliability.
• Enhances analytical accuracy by correcting for recovery losses, instrument fluctuations, and matrix effects.
• Suitable for isotope dilution techniques requiring the highest levels of quantitative precision.
• Compatible with multiple analytical platforms, including LC-MS, LC-MS/MS, GC-MS (after appropriate derivatization), NMR spectroscopy, and other advanced analytical techniques.
• Demonstrates excellent chemical stability under recommended storage conditions and resists isotopic exchange under neutral laboratory conditions.

Research and Industrial Significance

The widespread production and utilization of terephthalic acid in polyester manufacturing have increased the importance of reliable analytical standards for both industrial quality assurance and academic research. Deuterated Terephthalic Acid facilitates studies involving polymer degradation mechanisms, catalyst performance, reaction kinetics, microbial biodegradation, recycling processes, and environmental fate assessments. Researchers investigating PET recycling technologies, sustainable polymer chemistry, and circular economy initiatives frequently employ isotope-labeled compounds to obtain accurate mechanistic insights without altering native chemical behavior.

The compound also supports pharmaceutical and chemical laboratories performing trace-level impurity analysis, reference standard qualification, and analytical method transfer. Its excellent reproducibility and analytical consistency make it suitable for both routine quality control and advanced research applications.

Why Choose ResolveMass Laboratories Inc.?

ResolveMass Laboratories Inc. supplies premium-quality stable isotope-labeled compounds designed to meet the demanding requirements of analytical laboratories, pharmaceutical developers, polymer researchers, CROs, and academic institutions. Every isotope standard is manufactured with a strong emphasis on isotopic purity, batch consistency, analytical reliability, and regulatory compliance. Our deuterated reference standards are trusted for quantitative mass spectrometry, method validation, isotope dilution studies, and advanced scientific research worldwide.

Whether your laboratory is developing sensitive LC-MS methods, validating analytical procedures, monitoring polymer degradation, or performing environmental investigations, Deuterated Terephthalic-2,3,5,6-d4 Acid (CAS 60088-54-2) provides the accuracy, stability, and performance required for high-confidence analytical results.

Learn more about Deuterated Chemicals through,

• Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends
• Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 
• ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States.
• Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 
• How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 
• How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 
• Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 
• Custom Synthesis of Deuterated Chemicals