Diazinon-d5 (benzylic and aromatic) CAS No.: 100155-47-3

Diazinon-d5 (benzylic and aromatic) | CAS No.: 100155-47-3

Chemical Name: Diazinon-d5 (benzylic and aromatic)
Molecular Formula: C12H11D10N2O3PS
Molecular Weight: 314.41 g/mol
Isotopic Labeling: Deuterium (D5) on benzylic and aromatic positions
Purity: ≥ 98% (isotopic and chemical purity confirmed by NMR and GC-MS)

Product Overview

Diazinon-d5 (benzylic and aromatic) is a deuterium-labeled analog of Diazinon, a well-known organophosphorus insecticide widely used for controlling soil, seed, and foliage pests. The incorporation of five deuterium atoms in both the benzylic and aromatic regions of the molecule makes Diazinon-d5 a stable isotopic internal standard ideally suited for mass spectrometry-based quantitative analysis.

At ResolveMass Laboratories Inc., we offer high-purity isotopically labeled standards designed for trace-level quantification of pesticide residues, metabolites, and degradation products in complex matrices such as food, soil, and biological fluids. Diazinon-d5 serves as a reference standard in analytical method development, validation, and regulatory compliance testing in environmental and toxicological studies.

Chemical and Structural Information

Diazinon is chemically described as O,O-diethyl O-(2-isopropyl-6-methyl-4-pyrimidinyl) phosphorothioate, belonging to the class of organophosphorus compounds that act as acetylcholinesterase inhibitors.
In Diazinon-d5, five hydrogen atoms are replaced with deuterium, which does not alter the compound’s chemical behavior but significantly improves mass differentiation in analytical detection.

The labeled sites—both benzylic and aromatic positions—are strategically deuterated to minimize isotope exchange and provide robust isotopic stability during GC-MS or LC-MS/MS analysis. This structural feature ensures accurate quantification of unlabeled Diazinon by eliminating interference from isotopic overlap or fragmentation inconsistencies.

Applications and Uses

1. Analytical Reference Standard

Diazinon-d5 is extensively used as an internal standard in quantitative residue analysis by:

• Gas Chromatography-Mass Spectrometry (GC-MS)

• Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

• High-Resolution Mass Spectrometry (HRMS)

It provides enhanced precision and accuracy in quantifying Diazinon residues and metabolites in:

• Agricultural commodities and soil samples

• Environmental water testing

• Human and animal biomonitoring studies

2. Environmental and Food Safety Testing

Regulatory bodies like the EPA (Environmental Protection Agency) and EFSA (European Food Safety Authority) mandate residue monitoring for organophosphate pesticides. Diazinon-d5 enables accurate calibration curves, helping laboratories meet stringent Maximum Residue Limit (MRL) and Good Laboratory Practice (GLP) standards for pesticide analysis.

3. Metabolite and Degradation Pathway Studies

In environmental chemistry and toxicology research, Diazinon-d5 is employed to investigate:

• Photolytic and hydrolytic degradation pathways

• Transformation into oxon derivatives such as Diazoxon

• Metabolic profiling in plant and animal systems

Its stable isotopic signature allows researchers to trace the fate, transport, and biotransformation of Diazinon in various ecosystems with high fidelity.

4. Quality Control and Proficiency Testing

Analytical laboratories use Diazinon-d5 as a QC check standard or spiking solution in proficiency testing programs to verify extraction efficiency, recovery rate, and instrument performance during multi-residue pesticide analyses.

Analytical Characterization

Each batch of Diazinon-d5 from ResolveMass Laboratories undergoes comprehensive quality control testing including:

• ¹H NMR and ²H NMR to verify deuterium incorporation sites and isotopic purity

• GC-MS and LC-MS/MS for molecular integrity and purity profiling

• FTIR spectroscopy for functional group verification

• Elemental analysis for composition confirmation

We provide Certificates of Analysis (CoA) with detailed isotopic purity data and chromatographic profiles, ensuring full traceability and reproducibility.

Advantages of Using Diazinon-d5

• Excellent isotopic stability with minimal hydrogen-deuterium exchange

• Chemically identical to native Diazinon for ideal co-elution behavior

• Enhances accuracy and precision in quantitative mass spectrometry

• Facilitates compliance with international pesticide residue testing standards

• Reliable internal standard for environmental, agricultural, and toxicological applications

Ordering Information

• Catalogue Number: RM-DZ100155

• Pack Sizes: 10 mg | 25 mg | 100 mg

• Lead Time: 2–4 weeks (bulk quantities available on request)

• Custom Isotopic Labeling: Available for other organophosphates upon request

Safety and Handling

Diazinon-d5 should be handled by trained professionals familiar with organophosphorus compounds. Avoid contact with skin and eyes, and prevent inhalation of vapors. Use personal protective equipment and work under fume hood conditions. Dispose of material in accordance with local environmental regulations.

About ResolveMass Laboratories Inc.

ResolveMass Laboratories is a leading CRO specializing in stable isotope-labeled reference standards, impurity synthesis, and advanced analytical testing. Our expertise in custom synthesis and isotopic labeling supports global R&D in pharmaceuticals, agrochemicals, and environmental sciences.

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