Dodecanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 2687960-76-3

OVERVIEW  of  Dodecanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 2687960-76-3
Dodecanoyl-L-Carnitine-d₃ HCl (N-methyl-d₃) is a stable isotopically labeled analog of dodecanoyl-L-carnitine (also known as lauroyl-L-carnitine), a medium-chain acylcarnitine involved in mitochondrial fatty acid transport and β-oxidation. In this deuterated form, the three hydrogen atoms on the N-methyl group of the L-carnitine moiety are replaced with deuterium (d₃), introducing a +3 Da molecular weight shift without altering the compound’s biochemical characteristics.

This deuterated standard is primarily used in quantitative mass spectrometry (LC-MS/MS) and metabolomic profiling to accurately measure endogenous dodecanoyl-L-carnitine levels in biological matrices such as plasma, urine, and tissues. Dodecanoyl-L-Carnitine-d₃ HCl serves as a reference material for studies involving energy metabolism, mitochondrial function, and inborn errors of fatty acid oxidation.

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CHEMICAL INFORMATION

• Name: Dodecanoyl-L-Carnitine-d₃ hydrochloride

• Molecular Formula: C₁₉H₃₅D₃NO₄·HCl

• Molecular Weight: 382.98 g/mol

• CAS Number: 2687960-76-3

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated acylcarnitine; stable isotope-labeled lipid metabolite

• Stability: Stable under inert, dry, and cold conditions; hygroscopic in nature

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APPLICATIONS  of  Dodecanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 2687960-76-3

• Metabolomics and Lipidomics:
  Used as a stable isotope-labeled internal standard in targeted LC-MS/MS workflows for quantitative analysis of medium-chain acylcarnitines in biological samples. It ensures analytical accuracy in metabolic profiling and comparative quantification.

• Clinical and Biomedical Research:
  Plays a crucial role in diagnosing metabolic disorders such as medium-chain acyl-CoA dehydrogenase deficiency (MCADD) and related fatty acid oxidation defects, by serving as a calibration compound in acylcarnitine quantitation assays.

• Mass Spectrometry Calibration and Method Validation:
  Ideal for instrument calibration, method validation, and reproducibility testing in high-resolution mass spectrometry systems used in clinical biochemistry laboratories.

• Mitochondrial Function and Energy Metabolism Studies:
  Utilized in biochemical investigations exploring fatty acid uptake, oxidation, and energy regulation in mitochondria under labeled-tracer conditions.

• Quantitative Bioanalysis:
  Ensures accurate measurement of endogenous and exogenous acylcarnitines by compensating for ion suppression and extraction efficiency in complex matrices.

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ADVANTAGES  of  Dodecanoyl-L-Carnitine-d3 HCl (N-methyl-d3) | CAS 2687960-76-3

• High isotopic enrichment (≥ 98 atom % D) ensures reliable isotopic distinction and precision in quantitative MS analysis.

• Chemically identical to native dodecanoyl-L-carnitine, maintaining comparable retention time and ionization characteristics.

• Non-radioactive, stable, and safe for research and clinical laboratory applications.

• Excellent reproducibility and sensitivity in targeted metabolomic assays.

• Supports robust data normalization and quality control in lipidomics workflows.

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HANDLING, STORAGE & SAFETY

• Hazards: Generally regarded as non-toxic in analytical quantities; handle using standard laboratory safety procedures.

• Precautions: Use gloves, goggles, and protective clothing; prevent contact with moisture and direct sunlight.

• Storage: Store at –20 °C in an airtight, light-protected vial; avoid repeated freeze–thaw cycles.

• Disposal: Dispose of according to institutional and environmental regulations for non-hazardous organic compounds.

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QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.5 %

• Form: Crystalline or lyophilized powder

• Analytical Verification: Verified using LC-MS/MS and NMR (¹H and ²H spectra confirming deuterium incorporation).

• Documentation: Certificate of Analysis (CoA) provided, detailing isotopic purity, chemical integrity, and quality parameters.

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SUMMARY
Dodecanoyl-L-Carnitine-d₃ HCl (N-methyl-d₃) | CAS 2687960-76-3 is a deuterium-labeled medium-chain acylcarnitine standard used in metabolomic, lipidomic, and biochemical studies. Its stable isotopic labeling allows for precise quantification, method calibration, and comparative analysis of acylcarnitines in clinical and research settings. With high purity, isotopic enrichment, and excellent compatibility with LC-MS/MS workflows, this compound is a key analytical reagent for investigating fatty acid metabolism, mitochondrial energy pathways, and metabolic disorders involving lipid oxidation and transport.

 

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