Hexadecanoic-d31-acid | CAS 39756-30-4

OVERVIEW  of  Hexadecanoic-d31-acid | CAS 39756-30-4

Hexadecanoic-d₃₁-acid, also known as palmitic-d₃₁ acid, is the fully deuterated form of palmitic acid—a common long-chain saturated fatty acid (C₁₆H₃₂O₂). In this isotopologue, all 31 hydrogen atoms are replaced by deuterium (²H), yielding a molecular formula of C₁₆D₃₁O₂. The compound retains the chemical and physical properties of natural palmitic acid while offering a distinct isotopic signature, making it a valuable standard for mass spectrometry (MS), lipidomics, and metabolic tracing studies.

As a stable isotope-labeled analog, hexadecanoic-d₃₁-acid serves as an essential internal standard for quantitative analysis of fatty acids in biological and environmental samples. Its high isotopic enrichment enables differentiation from endogenous palmitic acid during GC-MS or LC-MS/MS analysis, ensuring precise quantification of lipid species across complex matrices.

CHEMICAL INFORMATION

• Name: Hexadecanoic-d₃₁ acid

• CAS Number: 39756-30-4

• Molecular Formula: C₁₆D₃₁O₂

• Molecular Weight: 287.62 g/mol

• Isotopic Enrichment: ≥98% D

• Chemical Class: Deuterated long-chain saturated fatty acid

• Solubility: Insoluble in water; soluble in organic solvents such as chloroform, methanol, and hexane

APPLICATIONS  of  Hexadecanoic-d31-acid | CAS 39756-30-4
Hexadecanoic-d₃₁-acid is widely used in biochemical, analytical, and environmental research due to its stability and isotopic precision:

• Quantitative Lipidomics:
  Used as an internal standard for quantifying palmitic acid and related saturated fatty acids in biological samples (plasma, tissue, lipid extracts) via GC-MS or LC-MS/MS.

• Metabolic Flux Analysis:
  Traces lipid biosynthesis, β-oxidation, and fatty acid elongation pathways in cell cultures and metabolic studies.

• Stable Isotope Labeling Studies:
  Provides a powerful tool for understanding lipid turnover, membrane incorporation, and fatty acid metabolism in vivo and in vitro.

• Environmental and Food Chemistry:
  Applied in the analysis of lipid contaminants, fatty acid residues, and food lipid composition with enhanced analytical accuracy.

• Calibration and Validation:
  Serves as a stable reference for method validation in mass spectrometry-based lipid quantification.

• Pharmaceutical and Nutritional Research:
  Facilitates studies involving lipid metabolism, drug–lipid interactions, and the effect of dietary fats on cellular processes.

ADVANTAGES  of  Hexadecanoic-d31-acid | CAS 39756-30-4

• High Isotopic Purity (≥98% D): Enables clear distinction between native and labeled compounds in analytical measurements.

• Enhanced Analytical Precision: Ideal for isotope dilution mass spectrometry (IDMS) applications, providing accurate quantification of palmitic acid.

• Excellent Thermal and Chemical Stability: Maintains structure under derivatization and chromatographic conditions.

• Direct Compatibility: Suitable for both GC-MS (as methyl ester derivatives) and LC-MS workflows.

• Biochemically Relevant: Mirrors the physiological behavior of native palmitic acid while providing isotopic traceability.

• Long-Term Storage Stability: Resistant to oxidation and degradation when stored under appropriate conditions.

SUMMARY
Hexadecanoic-d₃₁-acid (CAS 39756-30-4) is a high-purity, fully deuterated analog of palmitic acid, designed for advanced lipidomic and metabolic research. Its isotopic labeling allows accurate quantification and kinetic studies of fatty acid metabolism in biological systems, supporting both basic and applied research in biochemistry, metabolomics, nutrition, and clinical diagnostics.

At ResolveMass Laboratories Inc., Hexadecanoic-d₃₁-acid is supplied with exceptional isotopic enrichment and chemical purity, ensuring reliability for precision-based MS applications. Its performance in stable isotope tracing and quantitative analysis makes it a cornerstone reference standard for laboratories studying lipid pathways and metabolic regulation.

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