Imidazole-d4 | CAS 6923-01-9

OVERVIEW  of  Imidazole-d4 | CAS 6923-01-9

Imidazole-d₄ (CAS 6923-01-9) is a deuterium-labeled analog of imidazole in which four hydrogen atoms are substituted with deuterium. This isotopically labeled compound retains the chemical reactivity, polarity, and aromaticity of natural imidazole, while offering a measurable mass shift of +4 Da. Due to its stability and isotopic enrichment, Imidazole-d₄ is widely used in analytical chemistry, metabolic research, mass spectrometry (MS), and nuclear magnetic resonance (NMR) studies.

Its deuteration reduces background interference in spectroscopic analysis, allowing for more precise structural elucidation and quantitation. Additionally, Imidazole-d₄ serves as a reference standard and tracer molecule for reaction mechanism and isotope effect studies in organic and biological systems.

CHEMICAL INFORMATION

• Name: Imidazole-d₄

• Molecular Formula: C₃H₂D₄N₂

• Molecular Weight: 72.10 g/mol

• CAS Number: 6923-01-9

• Isotopic Labeling: Four deuterium atoms substituted on the imidazole ring

• Chemical Class: Deuterated heterocycle; stable isotope-labeled compound

• Stability: Chemically stable; resistant to normal handling conditions

APPLICATIONS  of  Imidazole-d4 | CAS 6923-01-9

• Mass Spectrometry (MS) Reference Standard:
  Imidazole-d₄ serves as an internal standard or calibration reference in GC-MS and LC-MS analysis, providing precise quantification and accurate isotope correction.

• NMR Spectroscopy:
  Its deuterated ring reduces proton interference, enhancing spectral clarity in ¹H NMR and aiding in ²H NMR characterization.

• Pharmaceutical Research:
  Used in drug metabolism studies and bioanalytical assays involving imidazole-containing drug intermediates or metabolites.

• Mechanistic and Isotope Effect Studies:
  Enables researchers to investigate reaction pathways, hydrogen–deuterium exchange kinetics, and enzyme-catalyzed transformations.

• Synthetic Chemistry:
  Acts as a labeled building block in the preparation of isotopically enriched imidazole derivatives for mechanistic and tracer studies.

ADVANTAGES  of  Imidazole-d4 | CAS 6923-01-9

• Provides a +4 Da mass shift, ideal for high-precision MS quantitation.

• Chemically identical to native imidazole, ensuring equivalent chromatographic and kinetic behavior.

• Non-radioactive and stable for long-term storage.

• Enhances signal resolution in NMR and MS applications.

• Ideal for use in bioanalytical calibration, drug stability, and environmental studies.

HANDLING, STORAGE & SAFETY

• Handling: Use standard laboratory protective equipment; avoid inhalation or prolonged contact.

• Storage: Keep tightly closed in a cool (2–8 °C), dry, and well-ventilated area.

• Safety: Low toxicity; may cause mild irritation upon contact or inhalation.

• Disposal: Dispose of in accordance with institutional and environmental regulations for organic laboratory chemicals.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Form: Solid (powder or crystals)

• Verification: Characterized by ¹H NMR, ²H NMR, and MS

• Documentation: Supplied with Certificate of Analysis (CoA) confirming isotopic enrichment and purity

SUMMARY
Imidazole-d₄ (CAS 6923-01-9) is a high-purity, deuterium-labeled isotopic standard designed for advanced mass spectrometry and NMR spectroscopy applications. Its excellent stability, high isotopic enrichment, and identical reactivity to native imidazole make it indispensable in pharmaceutical, biochemical, and mechanistic research. By providing a clear isotopic signature and superior analytical reproducibility, Imidazole-d₄ supports accurate quantitation, tracing, and molecular characterization in modern scientific and industrial research.