N-Nitrosodiethyl-d10-amine | CAS 1219794-54-3

OVERVIEW
N-Nitrosodiethyl-d₁₀-amine is a fully deuterated isotopologue of N-nitrosodiethylamine (NDEA), a well-known nitrosamine compound used extensively as an analytical reference standard. In this molecule, all ten hydrogen atoms of the two ethyl groups are replaced by deuterium, providing a molecular mass increase of +10 Da while preserving identical chemical and chromatographic properties to its non-deuterated counterpart.

This compound is primarily employed as a stable isotope-labelled internal standard for quantitative determination of nitrosamines in pharmaceuticals, environmental samples, and biological matrices. Its isotopic labeling allows for precise correction of matrix effects, enhancing the reliability and reproducibility of trace-level quantitation in GC-MS and LC-MS analyses.

CHEMICAL INFORMATION

• Name: N-Nitrosodiethyl-d₁₀-amine

• Molecular Formula: C₄D₁₀N₂O

• Molecular Weight: 108.18 g/mol

• CAS Number: 1219794-54-3

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated nitrosamine; secondary amine derivative

• Stability: Stable under recommended storage; sensitive to heat and light

APPLICATIONS  of  N-Nitrosodiethyl-d10-amine | CAS 1219794-54-3

• Analytical Standard for GC-MS / LC-MS:
  N-Nitrosodiethyl-d₁₀-amine serves as an isotopically labelled internal standard in gas and liquid chromatography–mass spectrometry for quantifying NDEA and other nitrosamines. Its deuterium substitution provides a clear mass difference (+10 Da), enabling precise and interference-free detection.

• Pharmaceutical Testing:
  Used in pharmaceutical quality control to monitor nitrosamine impurities that may form as by-products during drug synthesis or storage. Supports regulatory compliance with FDA, EMA, and ICH guidelines for nitrosamine limits.

• Environmental and Food Analysis:
  Applied in testing of environmental samples, including water, soil, and air, for nitrosamine contamination. It is also used in the analysis of processed foods and beverages where nitrosamines may form during curing or combustion.

• Toxicological and Biochemical Research:
  Facilitates studies on metabolic pathways, bioactivation, and degradation mechanisms of nitrosamines in biological systems. Its isotopic label enables tracking of compound fate and transformation kinetics.

• Stable Isotope Tracing:
  Ideal for use in mechanistic investigations involving nitrosation and oxidative degradation reactions.

ADVANTAGES  of  N-Nitrosodiethyl-d10-amine | CAS 1219794-54-3

• Fully deuterated (+10 Da) for accurate isotopic differentiation in MS analysis

• Chemically equivalent to NDEA, ensuring analytical reliability

• High isotopic enrichment (≥98 atom % D) and purity (≥98%)

• Suitable for trace-level quantification and regulatory testing

• Stable, non-radioactive, and compatible with standard analytical workflows

HANDLING

• Hazards: Classified as a potential carcinogen and toxic by inhalation, ingestion, or skin absorption. Handle with caution under well-ventilated conditions.

• Precautions: Use appropriate PPE including gloves, safety glasses, and lab coats. Avoid direct contact and prolonged exposure.

• Storage: Store in a cool, dark, and dry environment (2–8 °C), away from acids, oxidizing agents, and heat sources.

• Spill and Disposal: Clean spills promptly with absorbent material. Dispose of waste following institutional and environmental safety regulations.

Safety Note: The deuterated analog retains the toxicological properties of the non-deuterated NDEA and must be handled as a hazardous chemical.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.05 %

• Analytical Verification: ¹H NMR, ²H NMR, IR, and high-resolution mass spectrometry (HRMS)

• Appearance: Clear to pale yellow liquid

• Certificate of Analysis (COA): Provided for each batch verifying isotopic purity and chemical integrity

SUMMARY
N-Nitrosodiethyl-d₁₀-amine (CAS 1219794-54-3) is a high-purity, fully deuterated nitrosamine standard widely used in analytical and regulatory laboratories for mass spectrometric quantitation of NDEA and related compounds. Its high isotopic enrichment ensures exceptional analytical precision, making it a critical reference material in nitrosamine impurity testing, environmental monitoring, and toxicological research.