N,N-Dimethyl-d6-glycine HCl | CAS 347840-03-3

OVERVIEW  of  N,N-Dimethyl-d6-glycine HCl | CAS 347840-03-3

N,N-Dimethyl-d6-glycine hydrochloride is a deuterium-labeled derivative of dimethylglycine, a key intermediate in amino acid metabolism and methylation pathways. The substitution of deuterium in place of hydrogen provides a distinct isotopic signature that enhances analytical precision and differentiation in mass and NMR spectrometry.

As a stable isotope compound, N,N-Dimethyl-d6-glycine HCl is widely employed for quantifying and tracing biochemical reactions, studying metabolic flux, and validating analytical methodologies. The hydrochloride form enhances solubility and stability, making it highly suitable for aqueous and biological matrix analyses. At ResolveMass Laboratories Inc., each batch of this compound is synthesized under controlled isotopic labeling conditions to achieve the highest chemical and isotopic purity, ensuring dependable and reproducible analytical performance.

CHEMICAL INFORMATION

• Chemical Name: N,N-Dimethyl-d6-glycine hydrochloride

• Synonyms: Dimethyl-d6-glycine HCl; DMG-d6 HCl; N,N-Bis(methyl-d3)glycine hydrochloride

• CAS Number: 347840-03-3

• Molecular Formula: C₄D₆H₃NO₂·HCl

• Molecular Weight: 145.62 g/mol

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Structure: Deuterium atoms replace all six hydrogen atoms in the N-methyl groups of dimethylglycine; compound exists as a hydrochloride salt.

DESCRIPTION AND PROPERTIES
N,N-Dimethyl-d6-glycine hydrochloride combines the functional simplicity of a tertiary amine derivative with the analytical advantages of deuterium substitution. It serves as a stable isotopic marker for quantitative and comparative analysis due to its predictable mass shift of +6 Da relative to the non-deuterated analog.

The compound’s zwitterionic nature and hydrochloride stabilization make it ideal for studies in aqueous or biological systems, while its deuteration ensures minimal isotope exchange under standard analytical conditions. Its physicochemical stability and ease of ionization enhance sensitivity in MS-based detection methods.

APPLICATIONS  of  N,N-Dimethyl-d6-glycine HCl | CAS 347840-03-3

1. Internal Standard in Mass Spectrometry:
   N,N-Dimethyl-d6-glycine HCl is widely used as an internal standard for quantitative analysis of amino acids, methylated metabolites, and small molecules. Its mass difference ensures clear separation from native analytes in GC-MS and LC-MS spectra, improving data accuracy and reproducibility.

2. Stable Isotope Labeling in Metabolomics:
   In metabolomic and proteomic studies, deuterated dimethylglycine serves as a labeling reagent or reference compound for tracking metabolic flux and quantifying methylation intermediates. It provides precise isotopic distinction without affecting the native biochemical pathways.

3. Pharmaceutical and Biochemical Research:
   The compound is valuable for investigating methylation processes, enzyme kinetics, and choline metabolism. It also aids in validating analytical assays used for pharmacokinetic and bioanalytical studies.

4. NMR Spectroscopy Calibration:
   Deuterium labeling reduces proton interference and simplifies spectra, making it suitable for calibration and solvent suppression in proton and carbon NMR experiments. The predictable isotopic distribution assists in spectral interpretation and chemical shift assignments.

5. Kinetic Isotope Effect (KIE) Studies:
   The stronger C–D bond in the dimethyl groups enables examination of reaction mechanisms involving methyl transfer and oxidation. The compound’s isotopic integrity supports kinetic comparisons between deuterated and non-deuterated analogs.

6. Biochemical Pathway Tracing:
   Owing to its structural resemblance to endogenous dimethylglycine, this compound can serve as a safe, non-radioactive tracer for studies in amino acid metabolism, cellular methylation, and one-carbon biochemical cycles.

ANALYTICAL ADVANTAGES

• Distinct isotopic mass shift (+6 Da) for precise MS differentiation

• High isotopic purity minimizing background interference

• Excellent solubility and stability in aqueous and organic media

• Ideal for quantitative analysis and isotope dilution assays

• Enhances reproducibility in metabolomics and pharmacokinetic workflows

• Compatible with LC-MS, GC-MS, and NMR analytical systems

QUALITY AND MANUFACTURING
At ResolveMass Laboratories Inc., N,N-Dimethyl-d6-glycine HCl is produced via advanced deuteration and purification processes that ensure high isotopic enrichment and structural fidelity. Each batch undergoes comprehensive analytical validation using NMR spectroscopy, LC-MS, and elemental analysis to confirm purity, isotopic substitution pattern, and identity.

Our quality control standards meet or exceed research-grade and analytical-grade specifications, guaranteeing consistency, reproducibility, and compliance with modern laboratory requirements. We prioritize delivering stable isotope-labeled compounds with exceptional reliability for global pharmaceutical, environmental, and academic research applications.

CONCLUSION
N,N-Dimethyl-d6-glycine hydrochloride is an essential deuterated standard for analytical and biochemical research, offering unmatched accuracy, reproducibility, and isotopic integrity. Its high deuterium enrichment and excellent solubility make it a versatile compound for use in mass spectrometry calibration, metabolomic quantification, and isotopic labeling studies. Supported by the rigorous quality assurance of ResolveMass Laboratories Inc., this compound enables researchers to achieve precise, high-quality analytical results across diverse scientific disciplines.

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