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High Resolution Mass Spectrometry (HRMS) Analysis
At ResolveMass Laboratories Inc., our High Resolution Mass Spectrometry (HRMS) Analysis service offers precise molecular identification and structural confirmation across a wide range of scientific applications. By combining advanced HRMS platforms with the expertise of our seasoned analytical chemists, we deliver reproducible, validated data for research, development, and regulatory purposes.
Our laboratory’s capabilities extend beyond HRMS to include specialized services such as Extractables and Leachables Testing and Impurity Profiling, ensuring complete analytical support for complex formulations.
Why Partner with ResolveMass for HRMS Analysis?
- Advanced Orbitrap and Q-TOF analyzers for ultra-high mass accuracy.
- Experienced scientists with deep regulatory and pharmaceutical knowledge.
- Robust validation protocols to ensure data reliability.
- Fast, transparent reporting aligned with international compliance standards.
- Dedicated project support from initiation to result delivery.
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Understanding High Resolution Mass Spectrometry (HRMS)
High Resolution Mass Spectrometry (HRMS) is an advanced analytical approach designed to determine molecular masses with extreme precision — often accurate to within a few parts per million (ppm). Unlike conventional mass spectrometry, HRMS can differentiate between molecules with nearly identical masses, enabling unmatched specificity and structural clarity.
At ResolveMass, our HRMS systems — including Orbitrap and Time-of-Flight analyzers — provide both qualitative and quantitative insight for challenging matrices.
Learn more about our HRMS technology:
Our precision instrumentation allows accurate molecular formula determination, impurity detection, and unknown compound identification. HRMS is essential for pharmaceutical R&D, biological studies, and environmental analysis — ensuring clarity where traditional methods reach their limits.
Complementary services include:
Applications of HRMS Analysis
- Detection and structural elucidation of unknown compounds
- Pharmaceutical impurity and stability assessment
- Environmental contaminant screening (including PFAS)
- Characterization of peptides and biopharmaceuticals
- Proteomics and metabolomics studies
- Nitrosamine quantification and validation
Ensuring Data Precision and Scientific Integrity
Every dataset produced in our HRMS facility undergoes multi-tier validation, including instrument calibration, signal optimization, and spectral matching against certified libraries. Our analysts, each with advanced degrees in analytical chemistry, ensure scientific accuracy and full traceability.
We combine HRMS with complementary techniques such as LC-MS/MS and GC-MS, enabling comprehensive chemical profiling that meets global regulatory standards.
The Role of HRMS in Research and Quality Assurance
For researchers and organizations developing novel compounds or investigating product impurities, High Resolution Mass Spectrometry (HRMS) Analysis provides the confidence of precise molecular verification. Our laboratory’s expertise ensures results that strengthen scientific conclusions, support submissions, and drive innovation.
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Conclusion
At ResolveMass Laboratories Inc., we are committed to delivering precise, validated, and scientifically robust results through our High Resolution Mass Spectrometry (HRMS) Analysis services. Whether supporting drug development, environmental studies, or biomolecular research, our analytical expertise ensures that every result meets the highest standard of accuracy and reliability.
Frequently Asked Questions
The principle of High Resolution Mass Spectrometry (HRMS) is based on measuring the precise mass-to-charge ratio (m/z) of ions. HRMS instruments, such as Orbitrap or Time-of-Flight analyzers, can differentiate compounds with nearly identical molecular weights by resolving their exact masses to a few parts per million (ppm). This high accuracy enables precise molecular formula identification and structural analysis.
Interpreting HRMS data involves analyzing the high-resolution mass spectra to determine molecular composition and structural details. By comparing measured exact masses and isotope patterns with theoretical values, analysts can confirm compound identity, detect impurities, and elucidate unknown chemical structures. Interpretation relies on both accurate data and expert spectral analysis.
While traditional mass spectrometry (MS) provides information about molecular weight and structure, High Resolution Mass Spectrometry (HRMS) offers much higher mass accuracy and resolving power. HRMS can distinguish between compounds with very similar masses, making it essential for advanced applications such as impurity profiling, biomolecule analysis, and regulatory testing.
HRMS is widely used for precise identification, quantification, and structural characterization of molecules. It supports research in pharmaceuticals, environmental testing, metabolomics, proteomics, and forensic science. Because of its exceptional accuracy, HRMS is invaluable for detecting impurities, verifying formulations, and discovering biomarkers.
Reading a mass spectrum involves identifying the peaks that represent ionized fragments of the analyzed compound. Each peak corresponds to a specific mass-to-charge ratio (m/z), and the most intense peak, known as the base peak, indicates the dominant ion. By interpreting these peaks and their relative intensities, chemists can determine molecular structure and composition.
The acceptable mass error in High Resolution Mass Spectrometry typically falls within ±1 to 5 parts per million (ppm), depending on instrument type and calibration. This minimal error range ensures that molecular formulas can be determined with high confidence, providing reliable results for both qualitative and quantitative analyses.
Reference
- Hyde, A. (2020, April 27). High-resolution mass spectrometry: More than exact mass. Bioanalysis Zone. https://www.bioanalysis-zone.com/high-resolution-mass-spectrometry-bioanalysis/
- Wallace, M. A. G., & McCord, J. P. (2020). High-resolution mass spectrometry. In Breathborne Biomarkers and the Human Volatilome (Second Edition). Elsevier. https://www.sciencedirect.com/science/article/abs/pii/B9780128199671000165
- Hiragana, K. (2025). High-Resolution Mass Spectrometry in Analytical Chemistry-Beyond the Spectrum. Journal of Mass Spectrometry & Purification Techniques, 11, 286. https://www.longdom.org/open-access/highresolution-mass-spectrometry-in-analytical-chemistrybeyond-the-spectrum-107097.html