Isobutyric-d7-acid | CAS 223134-74-5

OVERVIEW  of  Isobutyric-d7-acid | CAS 223134-74-5

Isobutyric-d₇-acid is the fully deuterated analog of isobutyric acid (2-methylpropanoic acid), in which all seven hydrogen atoms are replaced by deuterium. This isotopic labeling provides a precise mass difference that is indispensable for quantitative mass spectrometry (MS), nuclear magnetic resonance (NMR), and isotope dilution analysis.

Deuterated organic acids like isobutyric-d₇-acid serve as highly reliable internal standards for analytical chemistry and biochemical research. Their use enables accurate quantification of native volatile fatty acids (VFAs), monitoring of metabolic fluxes, and evaluation of degradation or synthesis pathways involving branched-chain intermediates.

The compound retains the fundamental chemical behavior of its non-labeled counterpart while providing isotopic stability that eliminates analytical ambiguity—making it ideal for precision-based analytical laboratories and research in metabolomics, environmental science, and pharmaceutical testing.

---

CHEMICAL INFORMATION

• Name: Isobutyric-d₇-acid

• CAS Number: 223134-74-5

• Molecular Formula: C₄D₇O₂

• Molecular Weight: 95.15 g/mol

• Isotopic Enrichment: ≥98% D

• Chemical Class: Deuterated short-chain branched carboxylic acid

• Solubility: Miscible with most organic solvents; partially soluble in water

---

APPLICATIONS  of  Isobutyric-d7-acid | CAS 223134-74-5
Isobutyric-d₇-acid is a versatile and valuable isotope-labeled compound with broad research and analytical applications:

• Isotope Dilution Mass Spectrometry (IDMS):
  Used as an internal standard for accurate quantification of isobutyric acid and other short-chain carboxylic acids in biological and environmental matrices.

• Metabolomics and Biochemical Pathway Tracing:
  Supports studies on branched-chain amino acid (valine) metabolism and microbial fermentation pathways that yield short-chain fatty acids.

• Environmental and Food Analysis:
  Aids in the quantification of volatile organic acids in soil, air, and food samples, ensuring precise and reproducible detection.

• Analytical Method Development:
  Employed to validate GC-MS and LC-MS methods for volatile and semi-volatile acids, ensuring accuracy and linearity.

• Stable Isotope Research:
  Used to investigate hydrogen–deuterium exchange mechanisms and isotope effects in organic reactions and biological systems.

• Calibration and Quality Control:
  Serves as a stable calibration reference in routine laboratory analysis of complex mixtures containing branched-chain acids.

---

ADVANTAGES  of  Isobutyric-d7-acid | CAS 223134-74-5

• High Isotopic Purity (≥98% D): Ensures clear spectral separation from non-labeled isobutyric acid, minimizing analytical interference.

• Improved Analytical Accuracy: Ideal for quantitative analyses via isotope dilution techniques in GC-MS or LC-MS workflows.

• Thermal and Chemical Stability: Maintains structural integrity under typical chromatographic and sample preparation conditions.

• Reduced Odor Intensity: Easier handling compared to non-deuterated analogs due to decreased volatility and odor.

• Versatile Solvent Compatibility: Easily soluble in common organic solvents and suitable for diverse experimental setups.

• Reliable Standardization: Enhances reproducibility in metabolomics, environmental, and pharmaceutical studies.

---

SUMMARY
Isobutyric-d₇-acid (CAS 223134-74-5) is a deuterium-labeled, high-purity isotope standard widely used in analytical chemistry and metabolic research. Its seven-deuterium substitution provides a distinct mass signature that allows precise identification and quantification of isobutyric acid in complex mixtures using GC-MS or LC-MS.

Due to its stability, isotopic enrichment, and consistent analytical performance, Isobutyric-d₇-acid is a preferred choice for laboratories conducting isotope dilution quantification, metabolic profiling, and quality control testing.

At ResolveMass Laboratories Inc., this compound exemplifies our commitment to delivering high-quality deuterated standards designed for advanced research, ensuring reliability and reproducibility across analytical platforms.

 

Learn more about,

Deuterated Polymers: A Cornerstone Guide to Synthesis, Applications, and Future Trends

Availability of All the Deuterated Chemicals at ResolveMass Laboratories Inc. 

ResolveMass Laboratories: Leading Deuterated Chemical Synthesis Company in the United States. 

Deuterated Internal Standards for LC-MS: Selection & Custom Synthesis 

How to Choose the Right Deuterated Labelled Chemical Synthesis Company in Canada 

How to Choose the Right Deuterium Labelled Compounds Supplier for Your Lab 

Deuterium-Labelled Compounds — Synthesis, Applications & Ordering 

Custom Synthesis of Deuterated Chemicals